1887

Microbiology Society logo

Volume 138, Issue 2

Variable cross-reactivity of lipopolysaccharide-core-specific monoclonal antibodies and its possible relationship with serotype Free

Abstract

SUMMARY: The core region of lipopolysaccharide (LPS) was analysed by four LPS-core-specific human monoclonal antibodies (mAbs; FK-2E7, MH-4H7, OM-1D6 and NM-3G8). Reactivity of these mAbs to about 180 strains was tested. FK-2E7 bound to strains of Homma serotype E and I at a frequency of about 90%, to strains of serotype M at about 50%, and to strains of serotype A and G at about 30%. MH-4H7 bound to strains of serotype A, F, G, H, K and M at a high frequency (45-87%), but did not bind to any strains of serotype B, C, E and I. OM-1D6 and NM-3G8 bound to strains in a nearly serotype-specific manner. OM-1D6 reacted with all strains of serotype G so far tested, and a few strains of serotype M. Furthermore, L-rhamnose in the LPS core of serotype G was an immunodominant sugar recognized by OM-1D6 as an epitope. NM-3G8 bound to only a few strains of serotype B and M. The variable reactivity of these mAbs suggests that antigenic heterogeneity of the LPS core is somewhat related with (O-polysaccharide-based) serotype. Among these mAbs, MH-4H7 and OM-1D6 showed a high level of protective activity against in an experimental infection model using normal mice. protective activity was shown to be closely related to binding activity to whole cells as determined by agglutination and flow cytometry, but not ELISA.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
© Society for General Microbiology 1992
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-138-2-289
1992-02-01
2024-04-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/138/2/mic-138-2-289.html?itemId=/content/journal/micro/10.1099/00221287-138-2-289&mimeType=html&fmt=ahah

References

  1. Brade H., Galanos C. 1983; Common lipopolysaccharide specificity: new type of antigen residing in the inner core region of Sand R-form lipopolysaccharides from different families of Gram-negative bacteria. Infection and Immunity 42:250–256
     [Google Scholar]
  2. Brade L., Brade H. 1985; A 28000-dalton protein of normal mouse serum binds specifically to the inner core region of bacterial lipopolysaccharide. Infection, and Immunity 50:687–694
     [Google Scholar]
  3. Drewry D. T., Symes K. C, Gray G. W., Wilkinson S. G. 1975; Studies of polysaccharide fractions from the lipopolysaccharide of Pseudomonas aeruginosa NCTC 1999. Biochemical Journal 149:93–106
     [Google Scholar]
  4. Evans M. E., Pollack M., Hardegen N. J., Koles N. L., Guelde G. & Chia J. K. S. 1990; Fluorescence-activated cell sorter analysis of binding by lipopolysaccharide-specific monoclonal antibodies to gram-negative bacteria. Journal of Infectious Diseases 162:148–155
     [Google Scholar]
  5. Fisher M. W., Devlin H. B., Gnabasik F. J. 1969; New immunotype schema for Pseudomonas aeruginosa based on protective antigens. Journal of Bacteriology 98:835–836
     [Google Scholar]
  6. Fomsgaard A., Conrad R. S., Galanos C, Shand G. H., Hoiby N. 1988; Comparative immunochemistry of lipopolysaccharides from typable and polyagglutinable Pseudomonas aeruginosa strains isolated from patients with cystic fibrosis. Journal of Clinical Microbiology 26:821–826
     [Google Scholar]
  7. Hancock R. E. W., Mutharia L. M., Chan L., Darveau R. P., Speert D. P., Pier G. B. 1983; Pseudomonas aeruginosa isolates from patients with cystic fibrosis: a class of serum-sensitive, nontypable strains deficient in lipopolysaccharide O side chains. Infection and Immunity 42:170–177
     [Google Scholar]
  8. Homma J. Y. 1976; A new antigenic schema and live-cell slide-agglutination procedure for the infrasubspecific serologic classification of Pseudomonas aeruginosa. Japanese Journal of Experimental Medicine 46:329–336
     [Google Scholar]
  9. Klebba P. E., Benson S. A., Bala S., Abdullah T., Reid J., Singh S. P., Nikaido H. 1990; Determinants of OmpF porin antigenicity and structure. Journal of Biological Chemistry 265:6800–6810
     [Google Scholar]
  10. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
     [Google Scholar]
  11. Liu P. V., Matsumoto H., Kusama H., Bergan T. 1983; Survey of heat-stable, major somatic antigens of Pseudomonas aeruginosa. International Journal of Systematic Bacteriology 33:256–264
     [Google Scholar]
  12. Luderitz O., Freudenberg M. A., Galanos C, Lehmann V., Rietschel E. T., Shaw D. H. 1982; Lipopolysaccharides of gram-negative bacteria. Current Topics in Membranes and Transport 17:79–151
     [Google Scholar]
  13. Nelson D., Neill W., Poxton I. R. 1990; A comparison of immunoblotting, flow cytometry and ELISA to monitor the binding of anti-lipopolysaccharide monoclonal antibodies. Journal of Immunological Methods 133:227–233
     [Google Scholar]
  14. Pollack M., Raubitschek A. A., Larrick J. W. 1987; Human monoclonal antibodies that recognize conserved epitopes in the core-lipid A region of lipopolysaccharides. Journal of Clinical Investigation 79:1421–1430
     [Google Scholar]
  15. Rietschel E. T., Brade H., Brade L., Kaca W., Kawahara K., Linder B., Luderitz O., Tomita T., Schade U., Seydel U., Zahringer U. 1985; Newer aspects of the chemical structure and biological activity of bacterial endotoxins. Progress in Clinical and Biological Research 189:31–50
     [Google Scholar]
  16. Rowe P. S. N., Meadow P. M. 1983; Structure of the core oligosaccharide from the lipopolysaccharide of Pseudomonas aeruginosa PAC1R and its defective mutants. European Journal of Biochemistry 132:329–337
     [Google Scholar]
  17. Teng N. N. H., Lam K. S., Riera F. C., Kaplan H. S. 1983; Construction and testing of mouse-human heteromyelomas for human monoclonal antibody production. Proceedings of the National Academy of Sciences of the United States of America 50499–506
  18. Terashima M., Uezumi I., Tomio T., Kato M., Irie K., Okuda T., Yokota S., Noguchi H. 1991; Protective human monoclonal antibody directed to the outer core region of Pseudomonas aeruginosa lipopolysaccharide. Infection and Immunity 59:1–6
     [Google Scholar]
  19. Tsai C.-M., Frasch C. E. 1982; A sensitive stain for detecting lipopolysaccharides in polyacrylamide gels. Analytical Biochemistry 119:115–119
     [Google Scholar]
  20. Uchida K., Mizushima S. 1987; A simple method for isolation of lipopolysaccharides from Pseudomonas aeruginosa and some other bacterial strains. Agricultural and Biological Chemistry 51:3107–3114
     [Google Scholar]
  21. Wilkinson S. G. 1983; Composition and structure of lipopolysaccharides from Pseudomonas aeruginosa. Reviews of Infectious Diseases 5:S941–S949
     [Google Scholar]
  22. Yokota S., Ochi H., Ohtsuka H., Kato M., Noguchi H. 1989; Heterogeneity of the L-rhamnose residues in the outer core of Pseudomonas aeruginosa lipopolysaccharide, characterized by human monoclonal antibodies. Infection and Immunity 57:1691–1696
     [Google Scholar]
  23. Yokota S., Kaya S., Noguchi H. 1990; Antigenic epitope in Pseudomonas aeruginosa lipopolysaccharide immunologically cross reactive with Escherichia coli 026 lipopolysaccharide. FEMS Microbiology Letters 68:245–248
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-138-2-289
Loading
Variable cross-reactivity of Pseudomonas aeruginosa lipopolysaccharide-core-specific monoclonal antibodies and its possible relationship with serotype
Microbiology 138, 289 (1992); https://doi.org/10.1099/00221287-138-2-289
/content/journal/micro/10.1099/00221287-138-2-289
/content/journal/micro/10.1099/00221287-138-2-289
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error