@article{mbs:/content/journal/micro/10.1099/00221287-139-11-2723, author = "Bishop, A. H. and Douglas, C. W. I. and Dorr, P. K. and White, P. J.", title = "Components of the wall and capsule of Bacillus megaterium NCIB 7581", journal= "Microbiology", year = "1993", volume = "139", number = "11", pages = "2723-2730", doi = "https://doi.org/10.1099/00221287-139-11-2723", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-139-11-2723", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "Walls of Bacillus megaterium NCIB 7581 consisted principally of peptidoglycan (55%, w/w) and a single acidic carbohydrate accessory polymer (about 30%, w/w), which was isolated from lysozyme-digests of the walls. Glucose and N-acetylglucosamine partly made up this polymer, but phosphate and uronic or aminouronic acids were absent from the polymer and the wall. Protein (about 10%, w/w) was present in the walls, even though incubation with trypsin was a step in their isolation. When released into solution, this protein gave a single band on gel-electrophoresis and could be digested by trypsin. The remainder of the material isolated as walls was poly β-hydroxybutyrate, which represented cytoplasmic contamination. Capsules were formed best by bacteria on solid media containing amino acids, at relatively low growth temperatures. The isolated capsular material was polypeptide.", }