1887

Abstract

sp. strain C-125 has been chosen as a model alkalophilic bacterium to understand how adaptation to growth at high pH is achieved. To aid genetic analysis, we have started characterization of its genome. By using the two infrequently-cutting restriction endonucleases, I and 8387I, in conjunction with pulsed-field electrophoretic techniques, the size of the genome was found to be 3·7 Mb. Southern blot analysis of single, double and partial digests of sp. strain C-125 DNA, using I-linking clones, gene probes and purified sp. strain C-125 restriction fragments, allowed a putative chromosome map to be constructed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-139-4-661
1993-04-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/139/4/mic-139-4-661.html?itemId=/content/journal/micro/10.1099/00221287-139-4-661&mimeType=html&fmt=ahah

References

  1. Birkelund S., Stephens R. S. 1992; Construction of physical and genetic maps of Chlamydia trachomatis serovar L2 by pulsed-field gel electrophoresis. Journal of Bacteriology 174:2742–2747
    [Google Scholar]
  2. Chen H., Kuspa A., Keseler I. M., Shimkets L. J. 1991; Physical map of the Myxococcus xanthus chromosome. Journal of Bacteriology 173:2109–2115
    [Google Scholar]
  3. Chow K.-C., Wong T.-F. 1988; Cloning and nucleotide sequence of the structural gene coding for Bacillus subtilis tryptophanyl tRNA synthetase. Gene 73:537–543
    [Google Scholar]
  4. Fonstein M., Zheng S., Haselkorn R. 1992; Physical map of the genome of Rhodobacter capsulatus SB 1003. Journal of Bacteriology 174:4070–4077
    [Google Scholar]
  5. Honda H., Kudo T., Horikoshi T. 1985; Molecular cloning and expression of the xylanase gene of alkalophilic Bacillus sp. strain C-125 in Escherichia coli. Journal of Bacteriology 161:784–785
    [Google Scholar]
  6. Horikoshi K. 1991 In Microorganisms in Alkaline Environments pp. 5–24 Tokyo: Kodansha;
    [Google Scholar]
  7. Itaya M., Tanaka T. 1991; Complete physical map of the Bacillus subtilis 168 chromosome constructed by a gene-directed mutagenesis method. Journal of Molecular Biology 220:631–648
    [Google Scholar]
  8. Kang S.-K., Kudo T., Horikoshi K. 1992; Molecular cloning and characterization of an alkalophilic Bacillus sp. C-125 gene homologous to Bacillus subtilis secY. Journal of General Microbiology 138:1365–1370
    [Google Scholar]
  9. Kolsto A.-B., Gronstad A., Oppegaard H. 1990; Physical map of the Bacillus cereus chromosome. Journal of Bacteriology 172:3821–3825
    [Google Scholar]
  10. Krause D. C., Mawn C. B. 1990; Physical analysis and mapping of the Mycoplasma pneumoniae chromosome. Journal of Bacteriology 172:4790–4797
    [Google Scholar]
  11. Kudo T., Hino M., Kitada M., Horikoshi K. 1990; DNA sequences required for the alkalophily of Bacillus sp. C-125 are located close together on its chromosomal DNA. Journal of Bacteriology 172:7282–7283
    [Google Scholar]
  12. Piggot P. J., Hoch J. A. 1989; Revised genetic map of Bacillus subtilis 168. In Regulation of Procaryotic Development pp. 1–41 Smith I., Slepecky R., Setlow P. Edited by Washington, DC: American Society for Microbiology;
    [Google Scholar]
  13. Römling U., Tümmler B. 1991; The impact of two-dimensional pulsed-field gel electrophoresis techniques for the consistent and complete mapping of bacterial genomes: refined physical map of Pseudomonas aeruginosa PAO. Nucleic Acids Research 19:3199–3206
    [Google Scholar]
  14. Römling U., Grothues D., Bautsch W., Tümmler B. 1989; A physical genome map of Pseudomonas aeruginosa PAO. EMBO Journal 8:4081–4089
    [Google Scholar]
  15. Saito H., Miura K. 1963; Preparation of transforming DNA by phenol treatment. Biochemica et Biophysica Acta 72:619–629
    [Google Scholar]
  16. Sakamoto Y.-I., Sutherland K. J., Tamaoka J., Kobayashi T., Kudo T., Horikoshi K. 1992; Analysis of the flagellin (hag) gene of alkalophilic Bacillus sp. C-125. Journal of General Microbiology 138:2159–2166
    [Google Scholar]
  17. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  18. Smith C. L., Condemine G. 1990; New approaches for physical mapping of small genomes. Journal of Bacteriology 172:1167–1172
    [Google Scholar]
  19. Stagier P., Bouvier J., Bonamy C., Szulmajister J. 1984; A developmental gene product of Bacillus subtilis homologous to the sigma factor of Escherichia coli. Nature; London: 312376–378
    [Google Scholar]
  20. Taylor D. E., Eaton M., Yan W., Chang N. 1992; Genome maps of Campylobacter jejuni and Campylobacter coli. Journal of Bacteriology 174:2332–2337
    [Google Scholar]
  21. Yan W., Taylor D. E. 1991; Sizing of the genome of Campylobacter coli strain UA417R. Gene 101:117–120
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-4-661
Loading
/content/journal/micro/10.1099/00221287-139-4-661
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error