RT Journal Article SR Electronic(1) A1 Nishiyama, Makoto A1 Suzuki, Junko A1 Kukimoto, Mutsuko A1 Ohnuki, Tatsuya A1 Horinouchi, Sueharu A1 Beppu, TeruhikoYR 1993 T1 Cloning and characterization of a nitrite reductase gene from Alcaligenes faecalis and its expression in Escherichia coli JF Microbiology, VO 139 IS 4 SP 725 OP 733 DO https://doi.org/10.1099/00221287-139-4-725 PB Microbiology Society, SN 1465-2080, AB The gene (nir) encoding the copper-containing nitrite reductase (NIR) of a denitrifying bacterium, Alcaligenes faecalis S-6, was cloned by a synthetic oligonucleotide-probing method. The nucleotide sequence of the cloned DNA fragment revealed the primary structure of the NIR precursor containing the N-terminal signal sequence for secretion. A nucleotide sequence, possibly recognized by a transcriptional regulator resembling FNR was found upstream of the structural gene. When the cloned gene was expressed in Escherichia coli under the control of the lac promoter at 37 °C, NIR was produced as large inclusion bodies and little activity was detected. When cultivation was at 20 °C, most of the NIR was detected in the soluble fraction and a significant portion of the protein was translocated into the periplasmic space, accompanied by removal of its signal sequence., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-139-4-725