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Volume 139, Issue 8

Detection of seven species of pathogenic leptospires by PCR using two sets of primers Free

Abstract

SUMMARY: Two sets of primers derived from genomic DNA libraries of serovars (strain RGA) and (strain 1051) enabled the amplification by PCR of target DNA fragments from leptospiral reference strains belonging to all presently described pathogenic species. The -derived primers (G1/G2) enabled amplification of DNA from and whereas the -derived primers (B64-I/B64-II) enabled the amplification of Southern blot and DNA sequence analysis revealed inter-species DNA polymorphism within the region spanned by primers G1 and G2 between and various other species. Using a mixture of primer sets G1/G2 and B64-I/B64-II, leptospires of serovars and were detected in serum samples collected from patients during the first 10 days after the onset of illness.

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© Society for General Microbiology, 1993
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1993-08-01
2024-04-27
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References

  1. Boom R., Sol C. J. A., Salimans M. M. M., Jansen C. L., Wertheim-Van Dillen P. M. E., Van Der Noordaa J. 1990; Rapid and simple method for purification of nucleic acids.. Journal of Clinical Microbiology 28:495–503
     [Google Scholar]
  2. Burstajn J. M., Grimpel E., Lukehart S. A., Norgard M. V., Radolf J. D. 1991; Sensitive detection of Treponema pallidum by using the polymerase chain reaction.. Journal of Clinical Microbiology 29:62–69
     [Google Scholar]
  3. CENTERS FOR DISEASE CONTROL 1981; Annual Summary 1981: reported morbidity and mortality in the United States.. Morbidity and Mortality Weekly Report 301–134
     [Google Scholar]
  4. Demmlbr G. J., Buffone J., Schimbor C. M., May R. A. 1988; Detection of cytomegalovirus in urine from newborn by using polymerase chain reaction DNA amplification.. Journal of Infectious Diseases 158:1177–1185
     [Google Scholar]
  5. Dikken H., Kmety E. 1978; Serological typing methods of leptospirosis.. Methods in Microbiology 11:259–293
     [Google Scholar]
  6. Van Eys G. J. J. M., Gravekamp C., Gerritsen M. J., Quint W., Cornelissen M. T. E., Ter Schegget J., Terpstra W. J. 1989; Detection of leptospires in urine by polymerase chain reaction.. Journal of Clinical Microbiology 27:2258–2262
     [Google Scholar]
  7. Van Eys G. J. J. M., Gerritsen M. J., Korver H., Schoone G. J., Kroon C. C. M., Terpstra W. J. 1991; Characterization of serovars of the genus Leptospira by DNA hybridization with Hardjobovis and Icterohaemorrhagiae probes with special attention to serogroup Sejroe.. Journal of Clinical Microbiology 29:1042–1048
     [Google Scholar]
  8. Faine S. 1982 In Guidelines for Control of Leptospirosis WHO Offset Publications no. 67 pp. 67–77 Geneva: WHO;
     [Google Scholar]
  9. Gerritsen M. J., Olyhoek T., Smits M. A., Bokhout B. A. 1991; Sample preparation method from polymerase chain reaction- based semiquantitative detection of Leptospira interrogans serovar hardjo serotype Hardjobovis in bovine urine.. Journal of Clinical Microbiology 29:2805–2808
     [Google Scholar]
  10. Gravekamp C., Van De Kemp H., Carrington D., Van Eys G. J. J. M., Everard C. O. R., Terpstra W. J. 1991; Detection of leptospiral DNA by PCR in serum from patients with copenhageni- infections.. In Leptospirosis. Proceedings of the Leptospirosis Research Conference 1990, The 75th Anniversary of the Discovery of Causal Organism of Weil’s Disease by Inada and Ido pp. 151–164 Edited by Kobayashi Y. Tokyo: University of Tokyo Press;
     [Google Scholar]
  11. Hanahan D., Meselson M. 1983; Plasmid screening at high colony density.. Methods in Enzymology 100:333
     [Google Scholar]
  12. Hartskeerl R. A., De Wit M. Y. L., Klatser P. R. 1989; Polymerase chain reaction for the detection of Mycobacterium leprae. . Journal of General Microbiology 135:2357–2364
     [Google Scholar]
  13. Hay P. E., Clarke J. R., Strugne R. A., Taylor-Robinson D., Goldheimer D. 1990; Use of the polymerase chain reaction to detect DNA sequences specific to pathogenic treponemes in cerebral spinal fluid.. FEMS Microbiology Letters 68:428–432
     [Google Scholar]
  14. Herrmann J. L., Bellinger E., Perolat P., Baranton G., Saint Girons I. 1992; Pulsed-field gel electrophoresis of Notl digests of leptospiral DNA: a new rapid method of serovar identification.. Journal of Clinical Microbiology 30:1696–1702
     [Google Scholar]
  15. Hookey J. V. 1992; Detection of Leptospiraceae by amplification of 16S ribosomal DNA.. FEMS Microbiology Letters 90:267–274
     [Google Scholar]
  16. Johnson R. C., Harris V. G. 1967; Differentiation of pathogenic and saprophytic leptospires. I. Growth at low temperature.. Journal of Bacteriology 94:27–31
     [Google Scholar]
  17. Kmety E., Dikken H. 1988 International Committee on Systematic Bacteriology of the International Union of Microbiological Societies. Subcommittee on the Taxonomy of Leptospira. Revised list of Leptospira serovars I Groningen: University Press;
     [Google Scholar]
  18. Kogan S. C., Doherty M., Gitschier J. 1987; An improved method for prenatal diagnosis of genetic diseases by analysis of amplified DNA sequences. Application to hemophilia A.. New England Journal of Medicine 317:985–990
     [Google Scholar]
  19. Kolk A. H. J., Schuitema A. R. J., Kuipers S., Van Leeuwen J., Hermans P. W. M., Van Embden J. D. A., Hartskeerl R. A. 1992; Detection of Mycobacterium tuberculosis using polymerase chain reaction and a non-radioactive detection system.. Journal of Clinical Microbiology 30:2567–2575
     [Google Scholar]
  20. Korver H., Kolk A. H. J., Vingerhoed J., Van Leeuwen J., Terpstra W. J. 1988; Classification of the Icterohaemorrhagiae serogroup by monoclonal antibodies.. Israelian Veterinary Medicine 44:15–18
     [Google Scholar]
  21. Merien F., Amourioux P., Perolat P., Baranton G., Saint Girons I. 1992; Polymerase chain reaction for the detection of Leptospira spp. in clinical samples.. Journal of Clinical Microbiology 30:2219–2224
     [Google Scholar]
  22. Noordhoek G. T. 1991 Syphilis and Yaws. A molecular study to detect and differentiate pathogenic treponemes PhD thesis University of Utrecht; The Netherlands:
     [Google Scholar]
  23. Ou C., Kwok S., Mitchell S. W., Mack D. H., Sninsky J. J., Krebs J. W., Feorino P., Warfield D., Schochetman G. 1988; DNA amplification for direct detection of HIV-I in DNA of peripheral blood mononuclear cells.. Science 239:295–297
     [Google Scholar]
  24. Persing D. H. 1991; Polymerase chain reaction: trenches to benches.. Journal of Clinical Microbiology 29:1281–1285
     [Google Scholar]
  25. Prince A. M., Andrus L. 1992; PCR : how to kill unwanted DNA.. Biotechniques 12:358–360
     [Google Scholar]
  26. Ramadass P., Jarvis B. D. W., Corner J., Penny D., Marshall R. B. 1992; Genetic characterization of pathogenic Leptospira species by DNA hybridization.. International Journal of Systematic Bacteriology 42:215–219
     [Google Scholar]
  27. Saiki R. K., Gelfland D. H., Stoffel S., Scharf S. J., Higuchi R., Horn G. T., Mullis K. B., Erlich H. A. 1988; Primer- directed enzyme amplification of DNA with a thermostable DNA polymerase.. Science 239:487–491
     [Google Scholar]
  28. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn.. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press;
     [Google Scholar]
  29. Sanford J. P. 1984; Leptospirosis-time for a booster.. New England Journal of Medicine 310:524–525
     [Google Scholar]
  30. Segers R. P. A. M. . 1991 The molecular analysis of sphingomyelinase gene of Leptospiraceae PhD thesis University of Utrecht; The Netherlands:
     [Google Scholar]
  31. Sheldon W. H. 1953; Leptospiral antigen demonstrated by the fluorescent antibody technique in human muscle lesions of Leptospira icterohaemorrhagiae. . Proceedings of Society of Experimental Biology 84165–167
     [Google Scholar]
  32. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis.. Journal of Molecular Biology 98:503–517
     [Google Scholar]
  33. Terpstra W. J., Ligthart G. S., Schoone G. J. 1980; Serodiagnosis of human leptospirosis by Enzyme-Linked-Immuno- sorbent Assay (ELISA).. Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene A. 247:400–405
     [Google Scholar]
  34. Terpstra W. J., Jabboury-Postema J., Korver H. 1983; Immunoperoxidase staining of leptospires in blood and urine.. Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene A. 254:534–539
     [Google Scholar]
  35. Terpstra W. J., Schoone G. J., Ter SCHEGGET J. 1986; Detection of leptospiral DNA by nucleic acid hybridization with 32P- and biotin-labelled probes.. Journal of Medical Microbiology 22:23–28
     [Google Scholar]
  36. Terpstra W. J., Korver H., Schoone G. J., Van LEEUWEN J., Schonemann C. E., De Jonge-Aglibut S., Kolk A. H. J. 1987; Comparative classification of Leptospira serovars of the Pomona group by monoclonal antibodies and restriction endonuclease analysis.. Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene A 266:412–421
     [Google Scholar]
  37. Turner L. H. 1970; Leptospirosis III. Maintenance, isolation and demonstration of leptospires.. Transaction of the Royal Society of Tropical Medicine and Hygiene 64:623–646
     [Google Scholar]
  38. Wagenaar J. A., Gaastra W., Segers R. P. A. M., Van Der Zeist B. A. M. 1991; The detection of pathogenic leptospires by amplification of 16S rRNA sequences.. In Leptospirosis. Proceedings of the Leptospirosis Research Conference 1990, The 75th Anniversary of the Discovery of Causal Organism of Weil’s Disease by Inada and Ido p. 165 Edited by Kobayashi Y. Tokyo: University of Tokyo Press;
     [Google Scholar]
  39. White F. H., Ristic M. 1959; Detection of Leptospira pomona in guinea pig and bovine urine with fluorescein-labeled antibody.. Journal of Infectious Diseases 105:118–123
     [Google Scholar]
  40. De Wit M. Y. L., Faber W. R., Krieg S. R., Douglas J. T., Lucas S. B., Montreewasuwat N., Pattyn S. R., Hussein R., Ponnighaus J. M., Hartskeerl R. A., Klatser P. R. 1991; Application of a polymerase chain reaction for the detection of Mycobacterium leprae in skin tissues.. Journal of Clinical Microbiology 29:906–910
     [Google Scholar]
  41. Yanisch-Perron C., Vieira J., Messing J. 1985; Improved Ml3 phage cloning vectors and host strains : nucleotide sequences of the M13mpl8 and pUC19 vectors.. Gene 33:103–119
     [Google Scholar]
  42. Yasuda P. H., Steigerwalt A. G., Sulzer K. R., Kaufmann A. F., Rogers F., Brenner D. J. 1987; Deoxyribonucleic acid relatedness between serogroups and serovars in the family Leptospiraceae with proposals for seven new Leptospira species.. International Journal of Systematic Bacteriology 37:407–415
     [Google Scholar]
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Detection of seven species of pathogenic leptospires by PCR using two sets of primers
Microbiology 139, 1691 (1993); https://doi.org/10.1099/00221287-139-8-1691
/content/journal/micro/10.1099/00221287-139-8-1691
/content/journal/micro/10.1099/00221287-139-8-1691
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