1887

Microbiology Society logo

Volume 139, Issue 9

Chlamydia trachomatis infection of cultured motile cells after uptake of chlamydiae from the substratum Free

Abstract

SUMMARY: The ability of motile cells to remove small inanimate particles from solid substrata is well documented. We show here that motile cells will pick up and internalize infectious particles of the obligate intracellular parasite when they are adherent to the substratum over which the host cells move. Two cell types were used to assess chlamydial uptake; a feeder independent human squamous cell carcinoma variant (AC3A cells) and the McCoy cell line. Purified chlamydial elementary bodies were attached to glass or collagen-coated glass by centrifugation. Suspensions of cells were then allowed to sediment on to the substrata to which chlamydiae had attached. Both types of cell picked up chlamydiae and transported them over their surface during the course of attachment and spreading. Stereoscopic images obtained by confocal microscopy demonstrated that chlamydiae were found mainly on the surface of non-spread cells. After the cells had spread on the substratum they began to move around forming tracks where the chlamydiae had been removed. Some cell-surface-attached chlamydiae were endocytosed and a proportion of these proliferated during the 48 h after plating. However, chlamydiae attached to the substratum lost infectivity by a simple exponential decay process within a few hours of incubation in the extracellular environment. Therefore, increasing numbers of non-viable organisms were probably endocytosed as the time of extracellular incubation increased. This mode of infection may be relevant to situations where cell migration occurs after damage to mucosal surfaces.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
© Society for General Microbiology 1993
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-139-9-2151
1993-09-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/139/9/mic-139-9-2151.html?itemId=/content/journal/micro/10.1099/00221287-139-9-2151&mimeType=html&fmt=ahah

References

  1. Abercrombie M., Heasysman J.E.M., Pegrum S.M. 1970; The locomotion of fibroblasts in culture. Ill Movements of particles on the dorsal surface of the leading lamella.. Experimental Cell Research 62:389–398
     [Google Scholar]
  2. Albrecht-Buehler G. 1976; Filopodia of spreading 3T3 cells, Do they have a substrate-exploring function?. Journal of Cell Biology 69:275–286
     [Google Scholar]
  3. Albrecht-Buehler G. 1977a; The phagokinetic tracks of 3T3 cells.. Cell 11:395–104
     [Google Scholar]
  4. Albrecht-Buehler G. 1977b; Daughter 3T3 cells, Are they mirror images of each other?. Journal of Cell Biology 72:593–603
     [Google Scholar]
  5. Albrecht-Buehler G., Goldman R.D. 1976; Microspike- mediated particle transport towards the cell body during early spreading of 3T3 cells.. Experimental Cell Research 97:329–339
     [Google Scholar]
  6. Albrecht-Buehler G., Lancaster R.M. 1976; A quantitative description of the extension and retraction of surface protrusions in spreading 3T3 mouse fibroblasts.. Journal of Cell Biology 71:370–382
     [Google Scholar]
  7. Allan I., Pearce J.H. 1982; Radiolabelling of Chlamydiapsittaci (strain guinea pig inclusion conjunctivitis) to high specific activity using14C-labelled amino acids.. FEMS Microbiology Letters 13:69–73
     [Google Scholar]
  8. Batteiger B.E, Fraiz J., Newhall W.J., Katz B.P., Jones R.B. 1989; Association of recurrent chlamydial infection with gonorrhoea.. Journal of Infectious Diseases 159:661–669
     [Google Scholar]
  9. Campbell S, Haynes P., Gump D, Allen T.D. 1988; An in vitro model of Chlamydia trachomatis infection in the regenerative phase of the menstrual cycle.. Journal of General Microbiology 134:2077–2087
     [Google Scholar]
  10. Campbell S., Richmond S.J., Yates P. 1989; The development of Chlamydia trachomatis inclusions within the host cell during interphase and mitosis.. Journal of General Microbiology 135:1153–1165
     [Google Scholar]
  11. Campbell S., Yates P.S., Waters F., Richmond S.J. 1991; Purification of Chlamydia trachomatis by a simple and rapid filtration method.. Journal of General Microbiology 137:1565–1569
     [Google Scholar]
  12. Dembo M., Harris A.K. 1981; Motion of particles adhering to the leading lamella of crawling cells.. Journal of Cell Biology 91:528–536
     [Google Scholar]
  13. Elsdale T., Bard J.B.L. 1972; Collagen substrate for the study of cell behaviour.. Journal of Cell Biology 54:626–637
     [Google Scholar]
  14. Gail M.H., Boone C.W. 1970; The locomotion of mouse fibroblasts in tissue culture.. Biophysics Journal 10:980–993
     [Google Scholar]
  15. Hanna L. 1971; Immunofluorescence in chronic TRIC infections of American Indians and Tunisians: influence of trauma and results of tests.. In Trachoma and Related Disorders pp. 461–468 Nichols R.L. Edited by Amsterdam: Excerpta Medica.;
     [Google Scholar]
  16. Harris A.K., Dunn G. 1972; Centripetal transport of particles attached on both surfaces of moving fibroblasts.. Experimental Cell Research 73:519–523
     [Google Scholar]
  17. Hilton A.L., Richmond S.J., Milne J.D., Hindley F., Clarke S.K.R. 1974; Chlamydia A in the female genital tract.. British Journal of Venereal Diseases 50:1–10
     [Google Scholar]
  18. Hodinka R.L., Davis C.H., Choong J., Wyrick P.B. 1988; Ultrastructural study of endocytosis of Chlamydia trachomatis by McCoy cells.. Infection and Immunity 56:1456–1463
     [Google Scholar]
  19. Howard L., Orenstein N.S., King N.W. 1974; Purification on renografin gradients of Chlamydia trachomatis grown in the yolk sac of eggs.. Applied Microbiology 27:102–106
     [Google Scholar]
  20. Jones B.R. 1975; The prevention of blindness from trachoma.. Transactions of the Ophthalmology Society of the United Kingdom 95:16–33
     [Google Scholar]
  21. McAuslan B. R., Reilly W. 1980; Endothelial cell phagokinesis in response to specific metal ions.. Experimental Cell Research 130:147–157
     [Google Scholar]
  22. Odland G., Ross R. 1968; Human wound repair. I. Epidermal regeneration.. Journal of Cell Biology 39:135–151
     [Google Scholar]
  23. Oriel J.D., Ridgway G.L. 1982; Epidemiology of chlamydial infection of the human genital tract: evidence for the existence of latent infections.. European Journal of Clinical Microbiology 1:69–75
     [Google Scholar]
  24. Prain C.J., Pearce J.H. 1989; Ultrastructural studies on the intracellular fate of Chlamydia psittaci (strain guinea pig inclusion conjunctivitis) and Chlamydia trachomatis (strain lymphogranuloma venereum 434): modulation of intracellular events and relationship with endocytic mechanism.. Journal of General Microbiology 135:2107–2123
     [Google Scholar]
  25. Reynolds D.J., Pearce J.H. 1991; Endocytic mechanisms utilized by chlamydiae and theirinfluence on induction of productive infection.. Infection and Immunity 59:3033–3039
     [Google Scholar]
  26. Sompolinsky D., Richmond S. 1974; Growth of Chlamydia trachomatis in McCoy cells treated with cytochalasin B.. Applied Microbiology 28:912–914
     [Google Scholar]
  27. Vretou E., Goswami P.C., Bose S.K. 1989; Adherence of multiple serovars of Chlamydia trachomatis to a common receptor on HeLa and McCoy cells is mediated by thermolabile protein(s).. Journal of General Microbiology 135:3229–3237
     [Google Scholar]
  28. Ward M.E., Clarke I.N. 1990; New perspectives in chlamydial biology and development.. In Chlamydial Infections pp. 3–14 Bowie W.R., Caldwell H.D., Jones R.P., Mardh P.-A., Ridgway G.L., Schachter J., Stamm W.E., Ward M.E. Edited by Cambridge: Cambridge University Press.;
     [Google Scholar]
  29. Ward M.E., Murray A. 1984; Control mechanisms governing the infectivity of Chlamydia trachomatis for HeLa cells: mechanisms of endocytosis.. Journal of General Microbiology 130:1765–1780
     [Google Scholar]
  30. Wyrick P.B., Choong J., Davis C.H., Knight S.T., Royal M.O., Maslow A.S., Bagnell C.R. 1989; Entry of genital Chlamydia trachomatis into polarised human epithelial cells.. Infection and Immunity 57:2378–2389
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-9-2151
Loading
Chlamydia trachomatis infection of cultured motile cells after uptake of chlamydiae from the substratum
Microbiology 139, 2151 (1993); https://doi.org/10.1099/00221287-139-9-2151
/content/journal/micro/10.1099/00221287-139-9-2151
/content/journal/micro/10.1099/00221287-139-9-2151
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error