A spore-lytic enzyme released from Bacillus cereus spores during germination
Makino, Shio and Ito, Norihito and Inoue, Toshihiko and Miyata, Shigeru and Moriyama, Ryuichi,, 140, 1403-1410 (1994),
doi = https://doi.org/10.1099/00221287-140-6-1403,
publicationName = Microbiology Society,
issn = 1350-0872,
abstract= The exudate of fully germinated spores of Bacillus cereus IFO 13597 in 0·25 M sodium phosphate buffer, pH 7·0, was found to contain a spore-lytic enzyme. This enzyme was found to cause loss of absorbance in coat-stripped spore suspensions and phase-darkening of the spores but had minimal activity on isolated peptidoglycan substrates. The enzyme was purified in an active form and identified as a 24 kDa protein which is either an amidase or a peptidase. The amino-terminal 19 residues had the following sequence: FSNQVIQRGASGEKVIELQ. The spore-lytic enzyme retained its activity in a medium of a relatively high ionic strength containing a non-ionic surfactant such as nonaethyleneglycol n-dodecyl ether. This activity was optimum at a salt concentration of about 30 mM in assay buffer at neutral pH. In contrast to the enzyme in a spore-bound form, the enzyme in solution was shown to be heat-sensitive and was readily inactivated by thiol reagents.,
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