1887

Abstract

An improved integrative cassette from plasmid pSAM2 has been constructed containing plasmid and genes but excluding the gene, which should result in increased stability by suppression of the excision reaction. This cassette was included in both suicide and thermosensitive plasmids and used for integration in Suicide plasmids containing this cassette integrated at a single site () in the chromosome. The sequence of the site has been determined and was identified as a putative tRNA gene. Thermosensitive plasmids containing the cassette integrated both at the same site and at other different sites, often giving rise to simultaneous integration at two sites. A second integration site () has been sequenced, which was located in the region encoding 16S rRNA of one of the operons of

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-143-10-3375
1997-10-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/143/10/mic-143-10-3375.html?itemId=/content/journal/micro/10.1099/00221287-143-10-3375&mimeType=html&fmt=ahah

References

  1. Ausubel F.M., Brent R., Kingston R.E., Moore D.D., Seidman J.G., Smith J.A., Struhl K. 1990 Current Protocols in Molecular Biology New York: Wiley;
    [Google Scholar]
  2. Bercovier H., Kafri O., Sela S. 1986; Mycobacteria possess a surprisingly small number of ribosomal rna genes in relation to the size of their genome. Biocbem Biopbys Res Commun 136:1136–1141
    [Google Scholar]
  3. Boccard F., Smokvina T., Pernodet J.L., Friedmann A., Guerineau M. 1989; Structural analysis of loci involved in psam2 site-specific integration in streptomyces . Plasmid 21:59–70
    [Google Scholar]
  4. Boccard F., Smokvina T., Pernodet J.L., Friedmann A., Guerineau M. 1989; The integrated conjugative plasmid psam2 of streptomyces ambofaciens is related to temperate bacteriophages. EMBO J 8:973–980
    [Google Scholar]
  5. Eiglmeier K., Honore N., Cole S.T. 1991; Towards the integration of foreign dna into the chromosome of mycobacterium leprae . Res Microbiol 142:617–622
    [Google Scholar]
  6. Guilhot C., Gicquel B., Martin C. 1992; Temperature-sensitive mutants of the mycobacterium plasmid pal5000. FEMS Microbiol Lett 77:181–186
    [Google Scholar]
  7. Guilhot C., Otal I., Van Rompaey I., Martin C., Gicquel B. 1994; Efficient transposition in mycobacteria: Construction of mycobacterium smegmatis insertional mutant libraries. J Bacteriol 176:535–539
    [Google Scholar]
  8. Kuhstoss S., Rao R.N. 1991; Analysis of the integration function of the streptomycete bacteriophage φC31. J Mol Biol 222:897–908
    [Google Scholar]
  9. Kuhstoss S., Richardson M.A., Rao R.N. 1989; Site-specific integration in streptomyces ambofaciens: Localization of integration functions in s. ambofaciens plasmid psam2. J Bacteriol 171:16–23
    [Google Scholar]
  10. Kuhstoss S., Richardson M.A., Rao R.N. 1991; Plasmid cloning vectors that integrate site-specifically in streptomyces spp. Gene 97:143–146
    [Google Scholar]
  11. Lee M.H., Pascopella L., Jacobs W.R. Jr, Hatfull G.F. 1991; Site-specific integration of mycobacteriophage l5: Integrationproficient vectors for mycobacterium smegmatis, mycobacterium tuberculosis, and bacille calmette-guerin. Proc Natl Acad Sci USA 88:3111–3115
    [Google Scholar]
  12. McFadden J. 1996; Recombination in mycobacteria. Mol Microbiol 21:205–211
    [Google Scholar]
  13. Martin G., Mazodier P., Mendiola M.V., Gicquel B., Smokvina T., Thompson G.J., Davies J. 1991; Site-specific integration of the streptomyces plasmid psam2 in. Mol Microbiol 5:2499–2502
    [Google Scholar]
  14. Mazodier P., Thompson C., Boccard F. 1990; The chromosomal integration site of the streptomyces element psam2 overlaps a putative trna gene conserved among actinomycetes. Mol Gen Genet 222:431–434
    [Google Scholar]
  15. Pernodet J.L., Simonet J.M., Guerineau M. 1984; Plasmids in different strains of streptomyces ambofaciens: Free and integrated form of plasmid psam2. Mol Gen Genet 198:35–41
    [Google Scholar]
  16. Rogall T., Wolters J., Flohr T., Bottger E.C. 1990; Towards a phylogeny and definition of species at the molecular level within the genus mycobacterium . Int J Syst Bacteriol 40:323–330
    [Google Scholar]
  17. Simonet J.M., Boccard F., Pernodet J.L., Guerineau M. 1987; Excision and integration of a self-transmissible replicon of streptomyces ambofaciens . Gene 59:137–144
    [Google Scholar]
  18. Smokvina T., Mazodier P., Boccard F., Thompson C.J., Guerineau M. 1990; Construction of a series of psam2-based integrative vectors for use in actinomycetes. Gene 94:53–59
    [Google Scholar]
  19. Smokvina T., Boccard F., Pernodet J.-L., Friedmann A., Guerineau M. 1991; Functional analysis of the streptomyces ambofaciens element psam2. Plasmid 25:40–52
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-143-10-3375
Loading
/content/journal/micro/10.1099/00221287-143-10-3375
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error