1887

Abstract

A neutral trehalase gene, from the human pathogenic yeast was isolated and characterized. An ORF of 2724 bp was identified encoding a predicted protein of 907 amino acids and a molecular mass of 104 kDa. A single transcript of approxymately 3·2 kb was detected by Northern blot analysis. Comparison of the deduced amino acid sequence of the gene product with that of the gene product revealed 57% identity. The gene was localized on chromosome 1 or R. A null mutant (Δ) was constructed by sequential gene disruption. Extracts from mutants homozygous for neutral trehalase deletion had only marginal neutral trehalase activity. Extracts from heterozygous mutants showed intermediate activities between extracts from the wild-type strain and from the homozygous mutants. The null mutant showed no significant differences in pathogenicity as compared to the wild-type strain in a mouse model of systemic candidiasis. This result indicates that the neutral trehalase of is not a potential target for antifungal drugs.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-143-12-3747
1997-12-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/143/12/mic-143-12-3747.html?itemId=/content/journal/micro/10.1099/00221287-143-12-3747&mimeType=html&fmt=ahah

References

  1. App H., Holzer H. 1989; Purification and characterization of neutral trehalase from the ABYS1 mutant.. J Biol Chem 264:17583–17588
    [Google Scholar]
  2. Arguelles J.C., Gacto M. 1985; Evidence for regulatory trehalase activity in Candida utilis.. Can J Microbiol 31:529–537
    [Google Scholar]
  3. Arnold W.N., McLellan M.N. 1975; Trehalose and glycogen levels during the initial stages of growth of Candida albicans.. Physiol Chem Pbys 7:369–380
    [Google Scholar]
  4. Asano N., Yamaguchi T., Kameda Y. 1987; Effect of validamycins on glycohydrolases of Rkizoctonia solani.. J Antibiot 4:526–532
    [Google Scholar]
  5. Barton R.C., Scherer S. 1993; Induced chromosome rearrangements and morphologic variations in Candida albicans.. J Bacterial 176:756–763
    [Google Scholar]
  6. Boeke J.D., LaCroute F., Fink G.R. 1984; A positive selection for mutants lacking orotidine-5’-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance.. Mol Gen Genet 197:345–346
    [Google Scholar]
  7. Carrillo D., Vincente-Soler J., Fernandez J., Soto T., Cansado J., Gacto M. 1995; Activation of cytoplasmic trehalase by cyclic- AMP-dependent and cyclic-AMP-independent signalling pathways in the yeast Candida utilis.. Microbiology 141:679–686
    [Google Scholar]
  8. Cole M.F., Bowen W.H., Zhao X., Cihlar R.L. 1995; Avirulence of Candida albicans auxotrophic mutants in a rat model of oropharyngeal candidiasis.. FEMS Microbiol Lett 126:177–180
    [Google Scholar]
  9. Fonzi W.A. 1993; Isogenic strain construction and gene mapping in Candida albicans.. Genetics 134:717–728
    [Google Scholar]
  10. Forstner G.G., Sabsin S.M., Isselbacher K.L. 1968; Rat intestinal microvillus membranes: purification and biochemical characterization.. Biochem J 106:381–390
    [Google Scholar]
  11. George S.G., Kenny A.J. 1973; Studies on the enzymology of purified preparations of brush border from rabbit kidney.. Biochem J 134:43–57
    [Google Scholar]
  12. Gutierrez C., Ardourel M., Bremer E., Middendorf A., Boos W., Ehmann U. 1989; Analysis and DNA sequence of the osmoregulated treA gene encoding the periplasmic trehalase of Escherichia coli.. Mol Gen Genet 217:347–354
    [Google Scholar]
  13. Hill E.P., Sussmann A.S. 1963; Purification and properties of trehalase(s) from Neurospora.. Arch Biochem Biophys 102:389–396
    [Google Scholar]
  14. Hohmann S., Van Dijck P., Luyten K., Thevelein J.M. 1994; The bypl-3 allele of the Saccharomyces cerevisiae GGS1/TPS1 gene and its multi-copy suppressor tRNA GLN (CAG): Ggsl/Tpsl protein levels restraining growth on fermentable sugars and trehalose accumulation.. Curr Genet 26:295–301
    [Google Scholar]
  15. Hottiger T., Boiler T., Wiemken A. 1987; Correlation of trehalose content and heat resistance in yeast mutants altered in the RAS/adenylate cyclase pathway: is trehalose a thermoprotectant?. FEBS Lett 220:113–115
    [Google Scholar]
  16. Killick K.A. 1985; Alterations in trehalase solubility during development in the cellular slime mould Dictyostelium dis- coideum.. J Gen Microbiol 131:273–278
    [Google Scholar]
  17. Kopp M., Müller H., Holzer H. 1993; Molecular analysis of the neutral trehalase gene from Saccharomyces cerevisiae.. J Biol Chem 268:4766–4774
    [Google Scholar]
  18. Kopp M., Nwaka S., Holzer H. 1994; Corrected sequence of the yeast neutral trehalase-encoding gene (NTFI1): biological implication.. Gene 150:403–404
    [Google Scholar]
  19. Kyosseva S.V., Kyossev Z.N., Elbein A.D. 1995; Inhibitors of pig kidney trehalase.. Arch Biochem Biophys 316:821–826
    [Google Scholar]
  20. Lloyd A.T., Sharp P.M. 1992; Evolution of codon usage patterns: the extent and nature of divergence between Candida albicans and Saccharomyces cerevisiae.. Nucleic Acids Res 20:5289–5295
    [Google Scholar]
  21. Luces T.M., Phaff H.J. 1952; Characteristics of trehalase in Candida tropicalis.. Antonie Leeuwenhoek 18:323–335
    [Google Scholar]
  22. Merdinger E., Lange C.F., Booker B.F. 1971; Isolation and identification of trehalase from Pullularia pullans.. J Bacteriol 106:1034–1035
    [Google Scholar]
  23. Mittenbuhler K., Holzer H. 1988; Purification and characterization of acid trehalase from the yeast suc2 mutant.. J Biol Chem 263:8537–8543
    [Google Scholar]
  24. Plempel M. 1984; Antimycotic activity of Bay N 7133 in animal experiments.. J Antimicrob Chemother 13:447–463
    [Google Scholar]
  25. Retief L.W., Hewitt P.H. 1973; Purification and properties of trehalase from the harvester termite Trinervitermes trinervoides.. Insect Biochem 3:345–352
    [Google Scholar]
  26. Rittenhouse J., Harrsch P.B., Kim J.N., Marcus F. 1986; Amino acid sequence of the phosphorylation site of yeast {Saccharomyces cerevisiae) fructose-1,6-bisphosphatase.. J Biol Chem 261:3939–3943
    [Google Scholar]
  27. Ruf J., Wacker H., James P., Maffia M., Seiler P., Galand G., von Kieckenbusch A., Semenza G., Mantei N. 1990; Rabbit small intestinal trehalase. Purification, cDNA cloning, expression, and verification of glycosylphosphatidylinositol anchoring.. J Biol Chem 265:15034–15039
    [Google Scholar]
  28. Sacktor B. 1968; Trehalase and the transport of glucose in the mammalian kidney and intestine.. Proc Natl Acad Sci USA 60:1007–1014
    [Google Scholar]
  29. Sambrook J., Fritsch E.F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2nd edn.. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  30. Sanger F., Nicklen S., Coulson A.R. 1977; DNA sequencing with chain-terminating inhibitors.. Proc Natl Acad Sci USA 74:5463–5467
    [Google Scholar]
  31. Soli D.R. 1986; The regulation of cellular differentiation in the dimorphic yeast Candida albicans. . Bioessays 5:5–11
    [Google Scholar]
  32. Swoboda R.K., Bertram G., Hollander H., Greenspan D., Greenspan J.S., Gow N.A.R., Gooday G.W., Brown A.J.P. 1993; Glycolytic enzymes of Candida albicans are nonubiquitous immunogens during candidiasis.. Infect Immun 61:4263–4271
    [Google Scholar]
  33. Swoboda R.K., Bertram G., Budge S., Gow N.A.R., Gooday G.W., Brown A.P.J. 1995; Structure and regulation of the HSP90 gene from the pathogenic fungus Candida albicans.. Infect Immun 63:4506–4514
    [Google Scholar]
  34. Takiguchi M., Niimi T., Su Z.-H., Yaginuma T. 1992; T rehalase from male accessory gland of an insect, Tenebrio molitor.. Biochem J 288:19–22
    [Google Scholar]
  35. Thevelein J.M. 1984; Regulation of trehalose mobilization in fungi.. Microbiol Rev 48:42–59
    [Google Scholar]
  36. Van Aelst L, Bulaya B. & 15 other authors 1993; Molecular cloning of a gene involved in glucose sensing in the yeast Saccbaromyces cerevisiae.. Mol Microbiol 8:927–943
    [Google Scholar]
  37. Van Solingen P., van der Plaat J.B. 1975; Partial purification of the protein system controlling the breakdown of trehalose in Bakers yeast.. Biochem Biopbys Res Commun 62:553–560
    [Google Scholar]
  38. Wiemken A. 1990; Trehalose in yeast, stress protectant rather than reserve carbohydrate.. Antonie Leeuwenhoek 58:209–217
    [Google Scholar]
  39. Wolfe K.H., Lohan J.E. 1994; Sequence around the centromere of Saccbaromyces cerevisiae chromosome II: similarity of CEN2 to CEN4.. Yeast 10:41–46
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-143-12-3747
Loading
/content/journal/micro/10.1099/00221287-143-12-3747
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error