RT Journal Article SR Electronic(1) A1 Cormack, Brendan P. A1 Bertram, Gwyneth A1 Egerton, Mark A1 Gow, Neil A. R. A1 Falkow, Stanley A1 Brown, Alistair J. P.YR 1997 T1 Yeast-enhanced green fluorescent protein (yEGFP): a reporter of gene expression in Candida albicans JF Microbiology, VO 143 IS 2 SP 303 OP 311 DO https://doi.org/10.1099/00221287-143-2-303 PB Microbiology Society, SN 1465-2080, AB The green fluorescent protein (GFP) of Aequorea victoria has been developed here as a reporter for gene expression and protein localization in Candida albicans. When wild-type (wt) GFP was expressed in C. albicans, it was not possible to detect fluorescence or a translation product for the wt protein. Since this was probably due in part to the presence of the non-canonical CTG serine codon in the Aequorea sequence, this codon was changed to the leucine codon TTG. C. albicans cells expressing this construct contained GFP mRNA but were non-fluorescent and contained no detectable translation product. Hence a codon-optimized GFP gene was constructed in which all of the 239 amino acids are encoded by optimal codons for C albicans. In this gene were also incorporated two previously identified mutations in the chromophore that increase GFP fluorescence. C. albicans cells expressing this yeast-enhanced GFP gene (yEGFP3) are fluorescent and contain GFP protein. yEGFP3 can be used as a versatile reporter of gene expression in C. albicans and Saccharomyces cerevisiae and the optimized GFP described here should have broad applications in these and other fungal species., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-143-2-303