1887

Microbiology Society logo

Volume 143, Issue 5

Isolation and characterization of a strong promoter element from the phage I19 using the gentamicin resistance gene () of Tn as reporter Free

Abstract

A promoter-probe shuttle plasmid (pGL7011) containing the promoterless aminoglycoside--acetyltransferase I gene () of Tn was used to isolate DNA fragments from phage I19 that possessed promoter activity in TK23. Analysis of gentamicin (Gm) resistance levels in and in TK23, and of mRNA levels in identified a fragment (F14) that exhibited a high level of promoter activity in both species. Subsequent analysis revealed that the promoter activity of SF14 (a subcloned fragment of F14) was about twice that of *, one of the strongest characterized actinomycete promoters. SF14 contained two tandemly arranged promoters, 14-1p and p14-llp, with overlapping and adjacent -10 and -35 regions, respectively. Both promoters appear to be recognized with different efficiencies by the major RNA polymerase holoenzyme (Es) of A3(2).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): in vitro transcription , mRNA quantification , phage I19 , promoter , S1 nuclease mapping and Streptomyces ghanaensis
© Society Society for General Microbiology 1997
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-143-5-1503
1997-05-01
2024-05-03
Loading full text...

Full text loading...

/deliver/fulltext/micro/143/5/mic-143-5-1503.html?itemId=/content/journal/micro/10.1099/00221287-143-5-1503&mimeType=html&fmt=ahah

References

  1. Arnold W., Pühler A. 1988; A familiy of high-copy-number plasmid vectors with single end-label sites for rapid nucleotide sequencing. Gene 70:171–179
     [Google Scholar]
  2. Bagdasarian M. M., Amann E., Lurz R., Rückert B., Bagdasarian M. 1983; Activity of the hybrid trp–lac (tac) promoter of Escherichia coli in Pseudomonas putida. Construction of broad-host-range, controlled-expression vectors. Gene 26:273–282
     [Google Scholar]
  3. Baylis H. A., Bibb M. J. 1988; Transcriptional analysis of the 16S rRNA gene of the rrnD gene set of Streptomyces coelicolor A3(2). Mol Microbiol 2:569–579
     [Google Scholar]
  4. Beck E., Ludwig G., Auerswald E. A., Reiss B., Schaller H. 1982; Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5. Gene 19:327–336
     [Google Scholar]
  5. Bibb M. J., Janssen G. R. 1986; Unusual features of transcription and translation of antibiotic resistance genes in antibiotic-producing Streptomyces. In Proceedings of the Fifth International Symposium on the Genetics of Industrial Microorganisms, pp.. 309–318 Edited by M. Alacevic, D. Hranueli & Z. Toman, Karlovac, Yugoslavia: Ognjen Prica Printing Works
    [Google Scholar]
  6. Bibb M. J., Findlay P. R., Johnson M. W. 1984; The relationship between base composition and codon usage in bacterial genes and its use for the simple reliable identification of proteincoding sequences. Gene 30:157–166
     [Google Scholar]
  7. Bibb M. J., White J., Ward J. M., Janssen G. R. 1994; The mRNA for the 23S rRNA methylase encoded by the ermE gene of Saccharopolyspora erythraea is translated in the absence of a conventional ribosome-binding site. Mol Microbiol 14:533–545
     [Google Scholar]
  8. Bourn W. R., Babb B. 1995; Computer assisted identification and classification of streptomycete promoters. Nucleic Acids Res 23:3696–3703
     [Google Scholar]
  9. Brown K. L., Wood S., Buttner M. 1992; Isolation and characterization of the major vegetative RNA polymerase of Streptomyces coelicolor A3 (2); renaturation of a sigma subunit using GroEL. Streptomyces coelicolor 6:1133–1139
     [Google Scholar]
  10. Bruton C. J., Guthrie E. P., Chater K. F. 1991; Phage vectors that allow monitoring of transcription of secondary metabolism genes in Streptomyces. Biotechnology 9:652–656
     [Google Scholar]
  11. Buttner M. J., Fearnley I. M., Bibb M. J. 1987; The agarase gene (dagA) of Streptomyces coelicolor A3 (2): nucleotide sequence and transcriptional analysis. Mol Gen Genet 20:101–109
     [Google Scholar]
  12. Buttner M. J., Chater K. F., Bibb M. J. 1990; Cloning, disruption and transcriptional analysis of three RNA polymerase sigma factor genes of Streptomyces coelicolor A3 (2). J Bacteriol 172:3367–3378
     [Google Scholar]
  13. Carrer H., Staub J. M., Maliga P. 1991; Gentamycin resistance in Nicotina conferred by AAC(3)-I, a narrow substrate specifity acetyltransferase. Plant Mol Biol 17:301–303
     [Google Scholar]
  14. Chang A. C. Y., Cohen S. N. 1978; Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the cryptic miniplasmid P15A. J Bacteriol 134:1141–1156
     [Google Scholar]
  15. Chater K. F. 1984; Morphological and physiological differentiation in Streptomyces. In Microbial Development, pp.. 89–115 Edited by R. Losick & L. Shapiro. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory
    [Google Scholar]
  16. Chater K. F. 1989; Multilevel regulation of Streptomyces development. Trends Genet 5:372–577
     [Google Scholar]
  17. Chater K. F., Bruton C. J., Plaskitt K. A., Buttner M. J., Mendez C., Helmann J. D. 1989; The developmental fate of S. coelicolor hyphae depends upon a gene product homologous with the motility σ factor of B. subtilis. Cell 59:133–143
     [Google Scholar]
  18. Clayton T. M., Bibb M. J. 1990; Streptomyces promoter-probe vectors that utilise the xylE gene of Pseudomonas putida. Nucleic Acids Res 18:1077
     [Google Scholar]
  19. Feinberg A. P., Vogelstein B. 1983; A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13
     [Google Scholar]
  20. Gentz R., Langner A. C. Y., Cohen S. N., Bujard H. 1981; Cloning and analysis of strong promoters is made possible by the downstream placement of a RNA termination signal. Proc Natl Acad Sci USA 78:4936–4940
     [Google Scholar]
  21. Hentschel C., Irminger J.-C., Bucher P., Birnstiel M. L. 1980; Sea urchin histone mRNA termini are located in the gene regions downstream from the putative regulatory sequences. Nature 285:147–151
     [Google Scholar]
  22. Hopwood D. A. 1988; Towards an understanding of gene switching in Streptomyces, the basis of sporulation and antibiotic production. The Leeuwenhoek Lecture 1987. Proc R Soc Lond B 235:121–138
     [Google Scholar]
  23. Hopwood D. A., Kieser T., Bibb M. J. 1983; Plasmids, recombination and chromosomal mapping in Streptomyces lividans 66. J Gen Microbiol 129:2257–2269
     [Google Scholar]
  24. Hopwood D. A., Bibb M. J., Chater K., Kieser T., Bruton C. J., Kieser H. M., Lydiate D. J., Smith C. P., Ward J. M., Schrempf H. 1985; Genetic Manipulation of Streptomyces: A Laboratory Manual. Norwich: John Innes Foundation..
    [Google Scholar]
  25. Ingram C., Brawner M., Youngman P., Westpheling J. 1989; XylE functions as an efficient reporter gene in Streptomyces spp.: use for the study of galPl, a catabolite-controlled promoter. J Bacteriol 171:6617–6624
     [Google Scholar]
  26. Jones R. L., Jaskula J. C., Janssen G. R. 1992; In vivo translational start site selection on leaderless mRNA transcribed from the Streptomyces fradiae aph gene. J Bacteriol 174:4753–4760
     [Google Scholar]
  27. Kieser T., Hopwood D. A., Wright H. M., Thompson C. J. 1982; pIJ101, a multi-copy broad-host range Streptomyces plasmid: functional analysis and development of DNA cloning vectors. Mol Gen Genet 185:223–238
     [Google Scholar]
  28. Labes G., Simon R., Wohlleben W. 1990; A rapid method for the analysis of plasmid content and copy number in various Streptomycetes grown on agar plates. Nucleic Acids Res 18:2197
     [Google Scholar]
  29. Leskiw B. K., Bibb M. J., Chater K. F. 1991a; The use of a rare codon specifically during development?. Mol Microbiol 5:2861–2867
     [Google Scholar]
  30. Leskiw B. K., Lawlor E. J., Fernandez-Abalos J. M., Chater K. F. 1991b; TTA codons in some genes prevent their expression in a class of developmental, antibiotic-negative Streptomyces mutants. Proc Natl Acad Sci USA 88:2461–2465
     [Google Scholar]
  31. Lonetto M. A., Brown K. L., Rudd K. E., Buttner M. J. 1994; Analysis of the Streptomyces coelicolor sigE gene reveals the existence of a subfamily of eubacterial RNA polymerase σ factors involved in the regulation of extracytoplasmic functions. Proc Natl Acad Sci USA 91:7573–7577
     [Google Scholar]
  32. McCue L. A., Kwak J., Babcock M. J., Kendrick K. E. 1992; Molecular analysis of sporulation of Streptomyces griseus. Gene 115:173–179
     [Google Scholar]
  33. Maniatis T., Fritsch E. F., Sambrook J. 1982; Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory..
    [Google Scholar]
  34. Maxam A. M., Gilbert W. 1980; Sequencing end-labelled DNA with base-specific chemical cleavages. Methods Enzymol 65:499–560
     [Google Scholar]
  35. Meselson M., Yuan R. 1968; DNA restriction enzyme from E. coli. Nature 217:1110–1114
     [Google Scholar]
  36. Muth G., Wohlleben W., Pühler A. 1988; The minimal replicon of the Streptomyces ghanaensis plasmid pSG5 identified by subcloning and Tn5 mutagenesis. Mol Gen Genet 211:424–429
     [Google Scholar]
  37. Muth G., NuBbaumer B., Wohlleben W., Pühler A. 1989; A vector system with temperature-sensitive replication for gene disruption and mutational cloning in streptomycetes. Mol Gen Genet 219:341–348
     [Google Scholar]
  38. Muth G., Farr M., Hartmann V., Wohlleben W. 1995; Streptomyces ghanaensis plasmid pSG5: nucleotide sequence analysis of the self-transmissible minimal replicon and characterization of the replication mode. Plasmid 33:113–126
     [Google Scholar]
  39. Nazarov V., Zatkovic P., Goddny A. 1990; New shuttle promoter-probe vectors for E. coli and Streptomycetes. Biotech-nol Lett 12:639–644
     [Google Scholar]
  40. Paget M. S. B., Hintermann G., Smith C. P. 1994; Construction and application of streptomycete promoter probe vectors which employ the Streptomyces glaucescens tyrosinase-encoding gene as reporter. Gene 146:105–110
     [Google Scholar]
  41. Passantino Z., Puglia A.-M., Chater K. 1991; Additional copies of the actII regulatory gene induce actinorhodin production in pleiotropic bld mutants of Streptomyces coelicolor A3(2). J Gen Microbiol 137:2059–2064
     [Google Scholar]
  42. Piret J. M., Chater K. F. 1985; Phage-mediated cloning of bldA, a region involved in Streptomyces coelicolor morphological development, and its analysis by genetic complementation. J Bacteriol 163:965–972
     [Google Scholar]
  43. Pótucková L., Kelemen G. H., Findlay K. C., Lonetto M. A., Buttner M. J., Kormanec J. 1995; A new RNA polymerase sigma factor, σF, is required for the late stages of morphological differentiation in Streptomyces spp. Mol Microbiol 17:37–48
     [Google Scholar]
  44. Rubens C. E., McNeill W. F., Farrar W. E. >Jr 1979; Transposable plasmid deoxyribonucleic acid sequence in Pseudomonas aeruginosa which mediates resistance to gentamicin and four other antimicrobial agents. J Bacteriol 139:877–882
     [Google Scholar]
  45. Rubens R. A. 1987; Genetic analysis of the gentamicin resistance region of pPHIJI and incorporation into a wide host range cloning vector. Plasmid 18:84–88
     [Google Scholar]
  46. Sambrook J., Fritsch E. F., Maniatis T. 1989; Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory..
    [Google Scholar]
  47. Sanger F., Coulson A. R. 1978; The use of thin acrylamide gels for DNA sequencing. FEBS Lett 87:107–110
     [Google Scholar]
  48. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467
     [Google Scholar]
  49. Schmitt-John T., Engels J. W. 1992; Promoter constructions for efficient secretion expression in Streptomyces lividans. Appl Microbiol Biotechnol 36:493–498
     [Google Scholar]
  50. Sharp P. A., Berk A. J., Berget S. M. 1980; Transcription maps of adenovirus. Methods Enzymol 65:750–768
     [Google Scholar]
  51. Shirahama T., Fumurai T., Okanishi M. 1981; A modified regeneration method for Streptomycete protoplasts. Agric Biol Chem 45:1271–1273
     [Google Scholar]
  52. Sohaskey C. D., Im H., Schauer A. T. 1992; Construction and application of plasmid- and transposon-based promoter-probe vectors for Streptomyces spp. that employ a Vibrio harveyi luciferase reporter cassette. J Bacteriol 174:367–376
     [Google Scholar]
  53. Strohl W. R. 1992; Compilation and analysis of DNA sequences associated with apparent streptomycete promoters. Nucleic Acids Res 20:961–974
     [Google Scholar]
  54. Summerton J., Atkinson T., Bestwick R. 1983; A rapid method for preparation of bacterial plasmids. Anal Biochem 133:79–84
     [Google Scholar]
  55. Sutcliffe J. G. 1979; Complete nucleotide sequence of the E. coli plasmid pBR322. Cold Spring Habor Symp Quant Biol 43:77–90
     [Google Scholar]
  56. Tanaka K., Shiina T., Takahashi H. 1991; Nucleotide sequence of genes hrdA, hrdC, and hrdD from Streptomyces coelicolor A3(2) having similarity to rpoD genes. Mol Gen Genet 229:334–340
     [Google Scholar]
  57. Vieira J., Messing J. 1982; The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268
     [Google Scholar]
  58. Ward J. M., Janssen G. R., Kieser T., Bibb M. J., Buttner M. J., Bibb M. J. 1986; Construction and characterisation of a series of multi-copy promoter-probe plasmid vectors for Streptomyces using the aminoglycoside phosphotransferase gene from TnJ as indicator. Mol Gen Genet 203:468–478
     [Google Scholar]
  59. Westpheling J., Ranes M., Losick R. 1985; RNA polymerase heterogeneity in Streptomyces coelicolor. Nature 313:22–27
     [Google Scholar]
  60. Wohlleben W., Arnold W., Bissonette L., Pelletier A., Tanguay A., Roy P. H., Gamboa G. C., Barry G. F., Aubert E., Davies J., Kagan S. A. 1989; On the evolution of Tn21-like multiresistance transposons: sequence analysis of the gene (aacCl) for gentamicin acetyltransferase-3-I (AAC(3)-I), another member of the Tn21- based expression cassette. Mol Gen Genet 217:202–208
     [Google Scholar]
  61. Wohlleben W., Muth G., Kalinowsky J. 1993; Genetic engineering of Gram-positive bacteria. Biotechnology 2:455–505
     [Google Scholar]
  62. Wright F., Bibb M. J. 1992; Codon usage in the G + C rich Streptomyces genome. Gene 113:55–65
     [Google Scholar]
  63. Yanisch-Perron G., Vieira J., Messing J. 1985; Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors. Gene 33:103–119
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-143-5-1503
Loading
Isolation and characterization of a strong promoter element from the Streptomyces ghanaensis phage I19 using the gentamicin resistance gene (aacC1) of Tn1696 as reporter
Microbiology 143, 1503 (1997); https://doi.org/10.1099/00221287-143-5-1503
/content/journal/micro/10.1099/00221287-143-5-1503
/content/journal/micro/10.1099/00221287-143-5-1503
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error