Catabolism of D-glucose by Pseudomonas putida U occurs via extracellular transformation into D-gluconic acid and induction of a specific gluconate transport system
Schleissner, Carmen and Reglero, Angel and Luengo, José M.,, 143, 1595-1603 (1997),
doi = https://doi.org/10.1099/00221287-143-5-1595,
publicationName = Microbiology Society,
issn = 1350-0872,
abstract= Pseudomonas putida U does not degrade D-glucose through the glycolytic pathway but requires (i) its oxidation to D-gluconic acid by a peripherally located constitutive glucose dehydrogenase (insensitive to osmotic shock), (ii) accumulation of D-gluconic acid in the extracellular medium, and (iii) the induction of a specific energy-dependent transport system responsible for the uptake of D-gluconic acid. This uptake system showed maximal rates of transport at 30 ° in 50 mM potassium phosphate buffer, pH 7.0. Under these conditions the Km calculated for D-gluconic acid was 6.7 μM. Furthermore, a different transport system, specific for the uptake of glucose, was also identified. It is active and shows maximal uptake rates at 35 ° in 50 mM potassium phosphate buffer, pH 6.0, with a K m value of 8.3 μM.,
language=,
type=