1887

Abstract

When grown in the presence of benzoic acid, was able to extrude [C]benzoic acid when a pulse of glucose was given to preloaded cells. While octanoic, sorbic, hexanoic, salicylic, butyric and propionic acids were also inducers, ethanol and acetic acid were not. The mechanism of extrusion required energy and prior growth in the presence of the inducers. Diethylstilbestrol, an inhibitor of ATPases, prevented benzoic acid extrusion. Propionic acid was not actively extruded in cells adapted to either benzoic or propionic acid, behaving as an appropriate probe to measure intracellular pH. Even though the extrusion mechanism was active, benzoic acid entered the cells by a simple diffusion mechanism.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-143-6-1877
1997-06-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/143/6/mic-143-6-1877.html?itemId=/content/journal/micro/10.1099/00221287-143-6-1877&mimeType=html&fmt=ahah

References

  1. Allen C. N., Harpur E. S., Gray T. J. B., Simmons N. L., Hirst B. H. 1990; Efflux of bis-carboxylethyl-carboxyfluorescein (BCECF) by a novel ATP-dependent transport mechanism in epithelial cells.. Biochem Biophys Res Commulz 172:262–267
    [Google Scholar]
  2. Balzi E., Goffeau A. 1994; Genetics and biochemistry of yeast multidrug resistance.. Biochim Biophys Acta 1187:152–162
    [Google Scholar]
  3. Breeuwer P., Drocourt J.-L., Rombouts F. M., Abee T. 1994; Energy-dependent, carrier-mediated extrusion of carboxyfluorescein from Saccharomyces cerevisiae allows rapid assessment of cell viability by flow cytometry.. Appl Environ Microbiol 60:1467–1472
    [Google Scholar]
  4. Cole M. B., Keenan M. H. J. 1987; Effects of weak acids and external pH on the intracellular pH of Zygosaccharomyces bailii, and its implications in weak-acid resistance.. Yeast 3:23–32
    [Google Scholar]
  5. Decottignies A., Kolaczkowski M., Balzi E., Goffeau A. 1994; Solubilization and characterization of the overexpressed PDR5 multidrug resistance nucleotide triphosphatase of yeast.. J Biol Chem 269:12797–12803
    [Google Scholar]
  6. Krebs H. A., Wiggins D., Stubbs M., Sols A., Bedoya F. 1983; Studies on the mechanism of the antifungal action of benzoate.. Biochem J 214:657–663
    [Google Scholar]
  7. Leão C., van Uden N. 1984; Effects of ethanol and other alkanols on passive proton influx in the yeast Saccharomyces cerevisiae. . Biochim Biophys Acta 774:43–48
    [Google Scholar]
  8. Loureiro-Dias M. C., Peinado J. M. 1984; Transport of maltose in Saccharomyces cerevisiae. Effect of pH and potassium ions.. Biochem J 222:293–298
    [Google Scholar]
  9. Loureiro-Dias M. C., Santos H. 1990; Effects of ethanol on Saccharomyces cerevisiae as monitored by in vivo 31P and 13C nuclear magnetic resonance.. Arch Microbiol 153:384–391
    [Google Scholar]
  10. Pampulha M. E., Loureiro-Dias M. C. 1989; Combined effect of acetic acid, pH and ethanol on intracellular pH of fermenting yeast.. Appl Microbiol Biotechnol 31:547–550
    [Google Scholar]
  11. Pampulha M. E., Loureiro-Dias M. C. 1990; Activity of glycolytic enzymes of Saccharomyces cerevisiae in the presence of acetic acid.. Appl Microbiol Biotechnol 34:375–380
    [Google Scholar]
  12. Piper P. W., Braley R., Calderon C. O., Kong T., Seymour I., Talreja K. 1995; Adaptation of yeast to weak organic acids; a specific stress response.. Yeast 11:S585
    [Google Scholar]
  13. Ramos S., Balbin M., Raposo M., Valle E., Pardo L. A. 1989; The mechanism of intracellular acidification induced by glucose in Saccharomyces cerevisiae. . J Gen Microbiol 135:2413–2422
    [Google Scholar]
  14. Rottenberg H. 1979; The measurement of membrane potential and ΔpH in cells, organelles and vesicles.. Methods Enzymol 55:547–569
    [Google Scholar]
  15. Salema M., Lolkema J. S., San RomSo M. V., Loureiro-Dias M. C. 1996; The proton motive force generated in Leuconostocoenos by L-malate fermentation.. J Bacteriol 178:3127–3132
    [Google Scholar]
  16. Serrano R. 1980; Effect of ATPase inhibitors on the proton pump of respiratory-deficient yeast.. Eur J Biochem 105:419–424
    [Google Scholar]
  17. Thomas D. S., Davenport R. R. 1985; Zygosaccharomyces bailii - a profile of characteristics and spoilage activities.. Food Microbiol 2:157–169
    [Google Scholar]
  18. van Uden N. 1967; Transport-limited fermentation and growth of Saccharomyres cereuisiae and its competitive inhibition.. Arch Mikrobiol 58:155–168
    [Google Scholar]
  19. van Uden N. 1989; Effects of alcohols on membrane transport in yeasts. Edited by N. van Uden. Boca Raton, FL: CRC Press.. In Alcohol Toxicity in Yeasts and Bacteria pp:135–146
    [Google Scholar]
  20. Verduyn C., Postma E., Scheffers W. A., van Dijken J. P. 1992; Effect of benzoic acid on metabolic fluxes in yeasts: a continuous-culture study on the regulation of respiration and alcoholic fermentation.. Yeast 8:501–517
    [Google Scholar]
  21. Warth A. D. 1977; Mechanism of resistance of Saccharomyces bailii to benzoic, sorbic and other weak acids used as food preservatives.. J Appl Bacteriol 43:215–230
    [Google Scholar]
  22. Warth A. D. 1988; Effect of benzoic acid on growth yield of yeasts differing in their resistance to preservatives.. Appl Environ Microbiol 54:2091–2095
    [Google Scholar]
  23. Warth A. D. 1989; Relationship among cell size, membrane permeability, and preservative resistance in yeast species.. Appl Enuiron Microbiol 55:2995–2999
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-143-6-1877
Loading
/content/journal/micro/10.1099/00221287-143-6-1877
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error