1887

Microbiology Society logo

Volume 143, Issue 6

Mutations to antibiotic resistance occur during the stationary phase in ATCC 8014 Free

Abstract

When ATCC 8014 was maintained in LCM broth (which consists of buffered tryptone and is sufficient to support the growth of some species of ) for long periods (120 d), viable bacteria persisted. Rifampicin-, streptomycin- and sodium-fusidate-resistant mutants were recovered from parallel LCM broth cultures following a stochastic pattern. Individual cultures appeared to yield mutants intermittently. One culture in particular yielded rifampicin-resistant colonies at a frequency of 1 in 100 viable bacteria after 20 d incubation and these persisted until the experiment was terminated at 115 d. In a separate experiment two parallel cultures yielded mutants resistant to low concentrations of streptomycin at a similar frequency. Using a chemostat it was shown that in continuous culture in LCM at slow growth rates the highest frequency of recovery of antibiotic-resistant mutants was achieved when the bacteria exhibited doubling times of 90 h or greater. The frequency of recovery of mutants was as high as 1 in 1000 viable bacteria. Thus, mutations to antibiotic resistance in ATCC 8014 can take place in the absence of measurable cell division. The data are consistent with the notion that populations of starved bacteria in stationary phase can be genetically dynamic.

  • Published Online:
Keyword(s): antibiotic resistance , Lactobacillus plantarum , mutagenesis and stationary phase
© Society Society for General Microbiology 1997
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-143-6-1941
1997-06-01
2024-05-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/143/6/mic-143-6-1941.html?itemId=/content/journal/micro/10.1099/00221287-143-6-1941&mimeType=html&fmt=ahah

References

  1. Ahrne S., Molin G. 1991; Spontaneous mutations changing the raffinose nietabolism of Lactobacillus plantarum .. Antonie LPeuwenhoek 60:87–93
     [Google Scholar]
  2. Anderson D.G., McKay L.L. 1983; Simple and rapid method for isolating large plasmid DNA from lactic streptococci.. Appl Environ Microbiol 46:549–552
     [Google Scholar]
  3. Aukrust T., Blom H. 1992; STransformation of Lactobacillus strains used in meat and vegetable fermentations. Food Res Int 25:253–261
     [Google Scholar]
  4. Aymerich M.T., Hugas M., Garriga M., Vogel R.F., Monfort J. M. 1993; Electro-transformation of meat lacto bacilli. Effect of several parameters on their efficiency of transformation.. J Appl Bticteriol 75:320–325
     [Google Scholar]
  5. Curragh H. J., Collins M.A. 1992; High levels of spontaneous drug resistance in Lactobacillus . J Appl Bticteriol 73:31–36
     [Google Scholar]
  6. Efthymiou C., Hansen C.A. 1992; The antigenic analysis of Lactobacillus acidophilus . J Infect Dis 110:258–267
     [Google Scholar]
  7. Foster P. L. 1992; Directed mutation : between unicorns and goats.. J Bacteriol 174:1711–1716
     [Google Scholar]
  8. Foster P. L. 1995; Adaptive mutation. In Population Genetics of Bacteria (Society for General Microbiology Symposium 52), pp. 13-30. Edited by S . Baumberg, J. P. W. Young, E. M. H. Wellington & J. R. Saunders. Cambridge :. Cambridge University Press.
     [Google Scholar]
  9. Groat R.G., Schultz J.E., Zychlinsky E., Bockman A., Matin A. 1986; Starvation proteins in Escherichia coli : kinetics of synthesis and role in starvation survival.. J Bacteriol 168:486–493
     [Google Scholar]
  10. Hall B.G. 1986; Spontaneous point mutations that occur more often when advantageous than when neutral. Genetics 126:5–16
     [Google Scholar]
  11. Koch A.L. 1971; The adaptive responses of Escherichia coli to a feast or a famine existence.. Adv Microb Physiol 6:147–217
     [Google Scholar]
  12. Konings W.N., Veldkamp H. 1971; Energy transduction and solute transport mechanisms in relation to environments occupied by microorganisms. In Microbes in their Natural Environments (Society for General Microbiology Symposium), Edited by J. H Slater, R. Whittenbury & J. W. T. Wimpenny. Cambridge :. Cambridge University Press. 34:153–186
     [Google Scholar]
  13. Kroll R. 1995; The direct epifluorescent filter technique (DEFT). In Diagnostic Bacteriology Protocols. Edited by J. Howard % D. M. Whitcombe. Totowa, New jersey. Humana Press. 46:107–112
     [Google Scholar]
  14. Kunji E.R.S., Ubbink T., Matin A., Poolman B., Konings W. N. 1995; Physiological responses of Lactococcus lactis ML3 to alternating conditions of growth and starvation. Arch Microbiol 159:372–379
     [Google Scholar]
  15. Lacoste L., Lacaille E.M., Brakier-Gingras L. 1977; The role of misrepair processes in the isolation of new types of streptomycin-resistant mutants in Escherichia coli .. Mol Gen Genet 157:313–318
     [Google Scholar]
  16. Lenski R.E. 1977; Evolution in experimental populations of bacteria. In Population Genetics of Bacteria (Society for General Microbiology Symposium). Editied by S. Baumberg, J. P. W. Young, E. M. H. Wellington % J. R. Saunders. Cambridge. Cambridge University Press. 52:193–215
     [Google Scholar]
  17. Lester W. 1972; ERifampin : a semisynthetic derivative of rifamycin - a prototype for the future.. Annu Rev Microbiol 26:88–102
     [Google Scholar]
  18. Mercenier A., Pouwels P.H, Chassy B.M. 1994; Genetic engineering of lactobacilli, leuconostocs and Streptococcus thermophilus . In Genetics and Biotechnology of Lactic Acid Bacteria . Edited by M. J. Gasson % W. M. de Vos. London :. Blackie Academic % Professional.252–293
     [Google Scholar]
  19. Morishita T., Deguchi Y., Yajima M., Sakurai T., Yura T. 1981; Multiple nutritional requirements of Lacto bacilli : genetic lesions affecting amino acid biosynthetic pathways. Bacteriol 148:64–71
     [Google Scholar]
  20. Prival M.J., Cebula T.A. 1992; Sequence analysis of mutations arising during prolonged starvation of Salmonella typhimurium .. Genetics 132:303–310
     [Google Scholar]
  21. Postgate J. 1989; A microbial way of death. New Sci 122(1665):43–47
     [Google Scholar]
  22. Reinheimer L.A., Morelli L., Bottazzi V., Suárez, V. 1995; Phenotypic variability among cells of Lactobacillus helveticus ATCC15807. Int Dairy J 5:97–103
     [Google Scholar]
  23. Ryan F.J. 1955; Spontaneous mutation in non-dividing bacteria. Genetics 40:726–738
     [Google Scholar]
  24. Scholten A.H., Feenstra M.H., Hamstra A.M. 1991; Public acceptance of foods from biotechnology.. Food Biotechnol 5:331–345
     [Google Scholar]
  25. Sharp R., O´Donnell A.G., Gilbert H.G., Hazlewood G.P. 1992; Growth and survival of genetically manipulated Lactobacillus plantarum in silage. Appl Environ Microbiol 58:2517–2522
     [Google Scholar]
  26. Sinha R.P. 1992; Plasmid incompatibility in Lactobacillus plantarum caTC2.. Curr Microbiol 25:219–223
     [Google Scholar]
  27. Smith K.C. 1992; Spontaneous mutagenesis : experimental, genetic and other factors.. Mutat Res 277:139–162
     [Google Scholar]
  28. Stahl F.W. 1988; A unicorn in the garden.. Nature 335:112–1113
     [Google Scholar]
  29. Stamer J.R. 1975; Recent developments in the fermentation of sauerkraut. In Lactic Acid Bacteria in Bezmages and Food. Edited by J. G. Carr, C. V. Cutting & G. C. Whiting. London. Academic Press.267–280
     [Google Scholar]
  30. Symonds N. 1989; Anticipatory mutagenesis ?. Nature 337:119–120
     [Google Scholar]
  31. Tai P.C., Davis B.D. 1985; The actions of antibiotics on the ribosome. In The Scientific Basis of Antimicrobial Chemotherapy (Society for General Microbiology Symposium). Edited by D. Greenwood & F. O’Grady. Cambridge:?. Nature 38:41–68
     [Google Scholar]
  32. Teuber M. 1985; Exploitation of genetically-modified niicroorganisms in the food industry. In The Release of Genetically Modified Micvo-organisms - REGEM 2 (Federation of European Microbiological Societies Symposium ). Edited by D. E. S. Stewart-Tull & M. Sussman. New York:. Plenum Press. 63:59–69
     [Google Scholar]
  33. Zambrano M.M., Kolter R. 1995; EChanges in bacterial cell properties in going from exponential growth to stationary phase. In Microbiological Quality Assurance: a Guide Towards Relevance and Reproducibility of Znocula. Edited by M. R. W. Brown & P. Gilbert. Boca Raton. CRC Press.21–30
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-143-6-1941
Loading
Mutations to antibiotic resistance occur during the stationary phase in Lactobacillus plantarum ATCC 8014
Microbiology 143, 1941 (1997); https://doi.org/10.1099/00221287-143-6-1941
/content/journal/micro/10.1099/00221287-143-6-1941
/content/journal/micro/10.1099/00221287-143-6-1941
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error