1887

Microbiology Society logo

Volume 144, Issue 2

Localization of denitrification genes on the chromosomal map of Pseudomonas aeruginosa Free

Abstract

Cleavage of chromosomal DNA from PAO by I and I has been used together with PFGE and Southern hybridization to establish the map location of the following principal denitrification genes: (encoding the large and small subunits of respiratory nitrate reductase), (cytochrome- nitrite reductase), (uroporphyrinogen-III methyltransferase for haem biosynthesis), (nitric-oxide reductase complex), (nitrous-oxide reductase) and (an outer-membrane protein and OprC homologue). The study also included several genes related to anaerobic or microaerophilic metabolism: (encoding the catalytic subunit of the periplasmic nitrate reductase), (catalytic subunit of the cytochrome- oxidase), (oxygen-independent coproporphyrinogen-III oxidase), an -like regulatory gene, and and (electron carriers azurin and ferredoxin, respectively). Genes necessary for denitrification are concentrated at 20 to 36 min on the chromosome, where they form three separate loci, the and gene clusters. Genomic DNA of ZoBell was also subjected to I restriction and Southern analysis to assign denitrification genes to individual fragments. A homologue of encoding a putative component of the Cu-processing apparatus for nitrous-oxide reductase was identified. In both and there is evidence for the linkage of () with and ; and for the presence of a gene.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): denitrification , Pseudomonas aeruginosa chromosome and Pseudomonas stutzeri
© Society for General Microbiology 1998
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-144-2-441
1998-02-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/144/2/mic-144-2-441.html?itemId=/content/journal/micro/10.1099/00221287-144-2-441&mimeType=html&fmt=ahah

References

  1. Arai H., Igarashi Y., Kodama T. 1995; Expression of the nir and nor genes for denitrification in Pseudomonas aeruginosa requires a novel CRP/FNR-related transcriptional regulator, DNR, in addition to ANR.. FEBS Lett 371:73–76
     [Google Scholar]
  2. de Boer A. P. N., Reijnders W. N. M., Kuenen J. G., Stouthamer A. H., van Spanning R. J. M. 1994; Isolation, sequencing and mutational analysis of a gene cluster involved in nitrite reduction in Paracoccus denitrificans . Antonie Leeuwenhoek 66:111–127
     [Google Scholar]
  3. de Boer A. P. N., van der Oost J., Reijnders W. N. M., Westerhoff H. V., Stouthamer A. H., van Spanning R. J. M. 1996; Mutational analysis of the nor gene cluster which encodes nitric-oxide reductase from Paracoccus denitrificans . Eur J Biochem 242:592–600
     [Google Scholar]
  4. Braun C., Zumft W. G. 1991; Marker exchange of the structural genes for nitric oxide reductase blocks the denitrification pathway of Pseudomonas stutzeri at nitric oxide.. J Biol Chem 266:22785–22788
     [Google Scholar]
  5. Braun C., Zumft W. G. 1992; The structural genes of the nitric oxide reductase complex from Pseudomonas stutzeri are part of a 30-kilobase gene cluster for denitrification.. J Bacteriol 174:2394–2397
     [Google Scholar]
  6. Chen W., Kuo T. 1993; A simple and rapid method for the preparation of gram-negative bacterial genomic DNA.. Nucleic Acids Res 21:2260
     [Google Scholar]
  7. Chomczynski P. 1992; One-hour downward alkaline capillary transfer for blotting of DNA and RNA.. Anal Biochem 201:134–139
     [Google Scholar]
  8. Clark M. A., Tang Y. J., Ingraham J. L. 1989; A NosA-specific bacteriophage can be used to select denitrification-defective mutants of Pseudomonas stutzeri . J Gen Microbiol 135:2569–2575
     [Google Scholar]
  9. Cuypers H., Zumft W. G. 1993; Anaerobic control of denitrification in Pseudomonas stutzeri escapes mutagenesis of an fnr-like gene.. J Bacteriol 175:7236–7246
     [Google Scholar]
  10. Engler-Blum G., Meier M., Frank J., Müller G. A. 1993; Reduction of background problems in non-radioactive Northern and Southern blot analyses enables higher sensitivity than 32P- based hybridizations.. Anal Biochem 210:235–244
     [Google Scholar]
  11. Farinha M. A., Ronald S. L., Kropinski A. M., Paranchych W. 1993; Localization of the virulence-associated genes pilA, pilR, rpoN, fliA, fliC, ent, and fbp on the physical map of Pseudomonas aeruginosa PAO1 by pulsed-field electrophoresis.. Infect Immun 61:1571–1575
     [Google Scholar]
  12. Galimand M., Gamper M., Zimmermann A., Haas D. 1991; Positive FNR-like control of anaerobic arginine degradation and nitrate respiration in Pseudomonas aeruginosa . J Bacteriol 173:1598–1606
     [Google Scholar]
  13. de Gier J. -W. L., Schepper M., Reijnders W. N. M. 9 other authors 1996; Structural and functional analysis of aa 3-type and cbb 3-type cytochrome c oxidases of Paracoccus denitrificans reveals significant differences in proton-pump design.. Mol Microbiol 20:1247–1260
     [Google Scholar]
  14. Ginard M., Lalucat J., Tümmler B., Römling U. 1997; Genome organization of Pseudomonas stutzeri and resulting taxonomic and evolutionary considerations.. Int J Syst Bacteriol 47:132–143
     [Google Scholar]
  15. Glockner A. B., Zumft W. G. 1996; Sequence analysis of an internal 9.72-kb segment from the 30-kb denitrification gene cluster of Pseudomonas stutzeri . Biochim Biophys Acta 1277:6–12
     [Google Scholar]
  16. van Hartingsveldt J., Stouthamer A. H. 1973; Mapping and characterization of mutants of Pseudomonas aeruginosa affected in nitrate respiration in aerobic or anaerobic growth.. J Gen Microbiol 74:97–106
     [Google Scholar]
  17. Hoitink C. W. G., Woudt L. P., Turenhout J. C. M., van de Kamp M., Canters G. W. 1990; Isolation and sequencing of the Alcaligenes denitrificans azurin-encoding gene: comparison with the genes encoding blue copper proteins from Pseudomonas aeruginosa and Alcaligenes faecalis . Gene 90:15–20
     [Google Scholar]
  18. Holloway B. W., Römling U., Tümmler B. 1994; Genomic mapping of Pseudomonas aeruginosa PAO.. Microbiology 140:2907–2929
     [Google Scholar]
  19. Jeter R. M., Sias S. R., Ingraham J. L. 1984; Chromosomal location and function of genes affecting Pseudomonas aeruginosa nitrate assimilation.. J Bacteriol 157:673–677
     [Google Scholar]
  20. Jüngst A., Braun C., Zumft W. G. 1991a; Close linkage in Pseudomonas stutzeri of the structural genes for respiratory nitrite reductase and nitrous oxide reductase, and other essential genes for denitrification.. Mol Gen Genet 225:241–248
     [Google Scholar]
  21. Jüngst A., Wakabayashi S., Matsubara H., Zumft W. G. 1991b; The nirSTBM region coding for cytochrome cd 1-dependent nitrite respiration of Pseudomonas stutzeri consists of a cluster of mono-, di-, and tetraheme proteins.. FEBS Lett 279:205–209
     [Google Scholar]
  22. Kawasaki S., Arai H., Kodama T., Igarashi Y. 1997; Gene cluster of dissimilatory nitrite reductase (nir) from Pseudomonas aeruginosa: sequencing and identification of a locus for heme d 1 biosynthesis.. J Bacteriol 179:235–242
     [Google Scholar]
  23. Köhler T., Harayama S., Ramos J. -L., Timmis K. N. 1989; Involvement of Pseudomonas putida RpoN sigma factor in regulation of various metabolic functions.. J Bacteriol 171:4326–4333
     [Google Scholar]
  24. Lee H. S., Abdelal A. H. T., Clark M. A., Ingraham J. L. 1991; Molecular characterization of nosA, a Pseudomonas stutzeri gene encoding an outer membrane protein required to make copper-containing N2O reductase.. J Bacteriol 173:5406–5413
     [Google Scholar]
  25. Liao X., Charlebois I., Ouellet C. 9 other authors 1996; Physical mapping of 32 genetic markers on the Pseudomonas aeruginosa PAO1 chromosome.. Microbiology 142:79–86
     [Google Scholar]
  26. Meade H. M., Long S. R., Ruvkun G. B., Brown S. E., Ausubel F. M. 1982; Physical and genetic characterization of symbiotic and auxotrophic mutants of Rhizobium meliloti induced by transposon Tn5 mutagenesis.. J Bacteriol 149:114–122
     [Google Scholar]
  27. Mokhele K., Tang Y. J., Clark M. A., Ingraham J. L. 1987; A Pseudomonas stutzeri outer membrane protein inserts copper into N2O reductase.. J Bacteriol 169:5721–5726
     [Google Scholar]
  28. Nordling M., Young S., Karlsson B. G., Lundberg L. G. 1990; The structural gene for cytochrome c 551 from Pseudomonas aeruginosa: the nucleotide sequence shows a location down-stream of the nitrite reductase gene.. FEBS Lett 259:230–232
     [Google Scholar]
  29. Nunoshiba T., deRojas-Walker T., Wishnok J. S., Tannenbaum S. R., Demple B. 1993; Activation by nitric oxide of an oxidative-stress response that defends Escherichia coli against activated macrophages.. Proc Natl Acad Sci USA 90:9993–9997
     [Google Scholar]
  30. Preisig O., Anthamatten D., Hennecke H. 1993; Genes for a microaerobically induced oxidase complex in Bradyrhizobium japonicum are essential for a nitrogen-fixing endosymbiosis.. Proc Natl Acad Sci USA 90:3309–3313
     [Google Scholar]
  31. Römling U., Tümmler B. 1991; The impact of two-dimensional pulsed-field gel electrophoresis techniques for the consistent and complete mapping of bacterial genomes: refined physical map of Pseudomonas aeruginosa PAO.. Nucleic Acids Res 19:3199–3206
     [Google Scholar]
  32. Römling U., Heuer T., Tümmler B. 1994; Bacterial genome analysis by pulsed field gel electrophoresis techniques.. Adv Electrophor 7:355–405
     [Google Scholar]
  33. Saeki K., Wakabayashi S., Zumft W. G., Matsubara H. 1988; Pseudomonas stutzeri ferredoxin: close similarity to Azotobacter vinelandii and Pseudomonas ovalis ferredoxins.. J Biochem 104:242–246
     [Google Scholar]
  34. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual 2nd edn Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
     [Google Scholar]
  35. Sawers R. G. 1991; Identification and molecular characterization of a transcriptional regulator from Pseudomonas aeruginosa PAO1 exhibiting structural and functional similarity to the FNR protein of Escherichia coli . Mol Microbiol 5:1469–1481
     [Google Scholar]
  36. Sias S. R., Stouthamer A. H., Ingraham J. L. 1980; The assimilatory and dissimilatory nitrate reductases of Pseudomonas aeruginosa are encoded by different genes.. J Gen Microbiol 118:229–234
     [Google Scholar]
  37. Siddiqui R. A., Warnecke-Eberz U., Hengsberger A., Schneider B., Kostka S., Friedrich B. 1993; Structure and function of a periplasmic nitrate reductase in Alcaligenes eutrophus H16.. J Bacteriol 175:5867–5876
     [Google Scholar]
  38. Sodergren E. J., DeMoss J. A. 1988; narI region of the Escherichia coli nitrate reductase (nar) operon contains two genes.. J Bacteriol 170:1721–1729
     [Google Scholar]
  39. Viebrock A., Zumft W. G. 1988; Molecular cloning, heterologous expression, and primary structure of the structural gene for the copper enzyme nitrous oxide reductase from denitrifying Pseudomonas stutzeri . J Bacteriol 170:4658–4668
     [Google Scholar]
  40. Vijgenboom E., Busch J. E., Canters G. W. 1995; Physiological role and expression of the blue copper protein azurin in Pseudomonas aeruginosa . J Inorg Biochem 59:720
     [Google Scholar]
  41. Vollack K. -U., Römling U., Zumft W. G. 1996; Mapping of denitrification genes from Pseudomonas aeruginosa by macro-restriction analysis.. Biospectrum 1996: 70 (abstract)
    [Google Scholar]
  42. Yannone S. M., Burgess B. K. 1997; Identification of a palindromic sequence that is responsible for the up-regulation of NADPH-ferredoxin reductase in a ferredoxin I deletion strain of Azotobacter vinelandii . J Biol Chem 272:14454–14458
     [Google Scholar]
  43. Ye R. W., Haas D., Ka J. -O., Krishnapillai V., Zimmermann A., Baird C., Tiedje J. M. 1995; Anaerobic activation of the entire denitrification pathway in Pseudomonas aeruginosa requires Anr, an analog of Fnr.. J Bacteriol 177:3606–3609
     [Google Scholar]
  44. Yoneyama H., Nakae T. 1996; Protein C (OprC) of the outer membrane of Pseudomonas aeruginosa is a copper-regulated channel protein.. Microbiology 142:2137–2144
     [Google Scholar]
  45. Zumft W. G. 1997; Cell biology and the molecular basis of denitrification.. Microbiol Mol Biol Rev 61:533–616
     [Google Scholar]
  46. Zumft W. G., Dreusch A., Löchelt S., Cuypers H., Friedrich B., Schneider B. 1992; Derived amino acid sequences of the nosZ gene (respiratory N2O reductase) from Alcaligenes eutrophus, Pseudomonas aeruginosa and Pseudomonas stutzeri reveal potential copper-binding residues: implications for the CuA site of N2O reductase and cytochrome-c oxidase.. Eur J Biochem 208:31–40
     [Google Scholar]
  47. Zumft W. G., Braun C., Cuypers H. 1994; Nitric oxide reductase from Pseudomonas stutzeri: primary structure and gene organization of a novel bacterial cytochrome bc complex.. Eur J Biochem 219:481–490
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-144-2-441
Loading
Localization of denitrification genes on the chromosomal map of Pseudomonas aeruginosa
Microbiology 144, 441 (1998); https://doi.org/10.1099/00221287-144-2-441
/content/journal/micro/10.1099/00221287-144-2-441
/content/journal/micro/10.1099/00221287-144-2-441
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error