1887

Abstract

Most strains of express IgG-binding activity and this binding has been considered to be solely mediated by protein A. However, the existence of a second gene in strain 8325-4 encoding an IgG-binding polypeptide was recently reported. This novel IgG-binding polypeptide was found after panning a shotgun phage display library, made from chromosomal DNA, against immobilized human IgG. The complete gene () encoding this novel IgG-binding protein, denoted protein Sbi, has now been cloned and sequenced. Analysis of other strains showed that this gene is not unique for strain 8325-4. The protein consists of 436 amino acids and exhibits an immunoglobulin-binding specificity similar to protein A. Furthermore, it is shown that Sbi is highly expressed in strain Newman 4, which shows that IgG-binding activity in can be mediated by proteins other than protein A.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-144-4-985
1998-04-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/micro/144/4/mic-144-4-0985.html?itemId=/content/journal/micro/10.1099/00221287-144-4-985&mimeType=html&fmt=ahah

References

  1. Bod£n M. K., Flock J.-l. (1994); Cloning and characterization of a gene for a 19 kDa fibrinogen-binding protein from Staphylococcus aureus.. Molecular Microbiology, 12:(4),599–606 [View Article]
    [Google Scholar]
  2. Boyle M. D. P. (1990) Bacterial Immunoμlobulin-binding Proteins, vol. vols 1 and 2 London:: Academic Press;
    [Google Scholar]
  3. Deisenhofer J. (1981); Crystallographic refinement and atomic models of human Fc fragment and its complex with fragment B of protein A from Staphylococcus aureus at 2-9- and 2-8-A resolution.. Biochemistry, 20:(9),2361–2370 [View Article]
    [Google Scholar]
  4. Easmon C. S. F., Adlam C. (1983), Staphylococci and Staphylococcal Infections vols vol. 1 and 2 London:: Academic Press;
    [Google Scholar]
  5. Forsgren A., Forsum U. (1970); Role of protein A in non-specific immunofluorescence of Staphylococcus aureus.. Infection and Immunity, 2:(4),387–391 [View Article]
    [Google Scholar]
  6. Forsgren A., Sjöquist J. (1966); Protein A, Pseudoimmune reaction with human y-μlobulin.. Journal of Immunology, 97:822–827
    [Google Scholar]
  7. Forsgren A., Nordstrom K., Philipson L., Sjoquist J. (1971); Protein A mutants of Staphylococcus aureus. Journal of Bacteriology, 107245–250
    [Google Scholar]
  8. Hoogenboom H. R., Griffiths A. D., Johnson K. S., Chiswell D. J., Hudson P. et al. (1991); Multi-subunit proteins on the surface of filamentous phage: methodologies for displaying antibody (Fab) heavy and light chains.. Nucleic Acids Research, 19:(15),4133–4137 [View Article]
    [Google Scholar]
  9. landolo J. J. (1989); Genetic analysis of extracellular toxins of Staphylococcus aureus.. Annual Review of Microbiology, 43:(1),375–302 [View Article]
    [Google Scholar]
  10. Jacobsson K., Frykberg L. (1995); Cloning of ligand-binding domains of bacterial receptors by phage display.. BioTechniques, 18:878–885
    [Google Scholar]
  11. Jdnsson K., Signets C., Muller H.-P., Lindberg M. (1991); Two different genes encode fibronectin binding proteins in Staphylococcus aureus. The complete sequence and characterization of the second gene. European Journal of Biochemistry, 2021041–1048
    [Google Scholar]
  12. Jonsson K., McDevitt D., Homonylo McGavin M., Patti J. M., Hook M. (1995); Staphylococcus aureus expresses a major histocompatibility complex class II analog.. Journal of Biological Chemistry, 270:(37),21457–21460 [View Article]
    [Google Scholar]
  13. Jonsson P., Lindberg M., Haraldsson I., Wadstrom T. (1985); Virulence of Staphylococcus aureus in a mouse mastitis model: studies of alpha hemolysin, coagulase, and protein A as possible virulence determinants with protoplast fusion and gene cloning. Infection and Immunity, 49765–769
    [Google Scholar]
  14. Kronvall G., Quie P. G., Williams R. C. Jr (1970); Quantification of staphylococcal protein A: determination of equilibrium constant and number of protein A residues on bacteria.. Journal of Immunology, 104:140–147
    [Google Scholar]
  15. Lindberg M., Sjdstrom J.-E., Johansson T. (1972); Transformation of chromosomal and plasmid characters in Staphylococcus aureus.. Journal of Bacteriology, 109:(2),844–847 [View Article]
    [Google Scholar]
  16. Ldfdahl S., Guss B., Uhlen M., Philipson L., Lindberg M. (1983); Gene for staphylococcal protein A.. Proc Natl Acad Sci USA, 80:(3),697–701 [View Article]
    [Google Scholar]
  17. Moks T., Abrahms£n L., Nilsson B., Heilman U., Sjdquist J. et al. (1986); Staphylococcal protein A consists of five IgG- binding domains.. European Journal of Biochemistry, 156:(3),637–643 [View Article]
    [Google Scholar]
  18. Navarre W. W., Schneewind O. (1994); Proteolytic cleavage and cell wall anchoring at the LPXTG motif of surface proteins in Gram-positive bacteria.. Molecular Microbiology, 14:(1),115–121 [View Article]
    [Google Scholar]
  19. Novick R. P. (1967); Properties of a cryptic high-frequency transducing phage in Staphylococcus aureus.. Virology, 33:(1),155–156 [View Article]
    [Google Scholar]
  20. Oskouin B., Stewart G. C. (1990); Repression and catabolite repression of lactose operon of S. aureus.. Journal of Bacteriology, 172:(7),3804–3812 [View Article]
    [Google Scholar]
  21. Park P. W., Rosenbloom J., Abrams W. R., Rosenbloom J., Mecham R. P. (1996); Molecular cloning and expression of the gene for elastin-binding protein (ebpS) in Staphylococcus aureus.. Journal of Biological Chemistry, 271:(26),15803–15809 [View Article]
    [Google Scholar]
  22. Patel A. H., Nowland P., Weavers E. D., Foster T. (1987); Virulence of protein A and alpha-toxin-deficient mutants of Staphylococcus aureus isolated by allele replacement. Infection and Immunity, 553103–3110
    [Google Scholar]
  23. Patti J. M., Allen B. L., McGavin M. J., Hodk M. (1994); MSCRAMM-mediated adherence of microorganisms to host tissue. Annual Review of Microbiology, 48585–617
    [Google Scholar]
  24. Rosendorf L. L., Kayser F. H. (1974); Transduction and plasmid deoxyribonucleic acid in a multiply antibiotic-resistant strain of Staphylococcus epidermidis.. Journal of Bacteriology, 120:(2),679–686 [View Article]
    [Google Scholar]
  25. Sambrook J., Fritsch E. F., Maniatis T. (1989) Molecular Cloning: a Laboratory Manual, 2nd edn.. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory;
    [Google Scholar]
  26. Schneewind O., Model P., Fischetti V. A. (1992); Sorting of protein A to the staphylococcal cell wall.. Cell, 70:(2),267–281 [View Article]
    [Google Scholar]
  27. Schneewind O., Fowler A., Faull K. F. (1995); Structure of cell wall anchor of surface proteins in Staphylococcus aureus.. Science, 268:(5207),103–106 [View Article]
    [Google Scholar]
  28. Uhl€n M., Guss B., Nilsson B., Gatenbeck S., Philipson L. et al. (1984); Complete sequence of the staphylococcal gene encoding protein A, a gene evolved through multiple duplications.. Journal of Biological Chemistry, 259:(3),1695–1702 [View Article]
    [Google Scholar]
  29. Wertman K. F., Wyman A. R., Botstein B. (1986); Host/vector interactions which affect the viability of recombinant phage lambda clones.. Gene, 49:(2),253–262 [View Article]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-144-4-985
Loading
/content/journal/micro/10.1099/00221287-144-4-985
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error