A second IgG-binding protein in Staphylococcus aureus
Zhang, Lihong and Jacobsson, Karin and Vasi, Jozsef and Lindberg, Martin and Frykberg, Lars,, 144, 985-991 (1998),
doi = https://doi.org/10.1099/00221287-144-4-985,
publicationName = Microbiology Society,
issn = 1350-0872,
abstract= Most strains of Staphylococcus aureus express IgG-binding activity and this binding has been considered to be solely mediated by protein A. However, the existence of a second gene in S. aureus strain 8325-4 encoding an IgG-binding polypeptide was recently reported. This novel IgG-binding polypeptide was found after panning a shotgun phage display library, made from chromosomal DNA, against immobilized human IgG. The complete gene (sbi) encoding this novel IgG-binding protein, denoted protein Sbi, has now been cloned and sequenced. Analysis of other S. aureus strains showed that this gene is not unique for strain 8325-4. The protein consists of 436 amino acids and exhibits an immunoglobulin-binding specificity similar to protein A. Furthermore, it is shown that Sbi is highly expressed in strain Newman 4, which shows that IgG-binding activity in S. aureus can be mediated by proteins other than protein A.,
language=,
type=