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Volume 144, Issue 8

16S rRNA gene sequences of ‘Candidatus Campylobacter hominis’, a novel uncultivated species, are found in the gastrointestinal tract of healthy humans Free

Abstract

Although some species are agents of gastroenteritis and periodontal disease in humans, little is known of the variety of campylobacters in the gastrointestinal tract of healthy individuals. This paper provides evidence for the existence of a previously undescribed, uncultivated species that may be a commensal in the healthy human gut. Saliva and faeces from 20 healthy individuals were examined by PCR assays specific for nine species of campylobacter and and for the genus as a whole. Genus-specific amplicons were produced from 19 of 20 saliva samples and from 18 of 20 faecal samples. species-specific amplicons were produced from 19 of 20 saliva samples and 3 of 20 faecal samples. The faecal samples were all PCR-negative for other species. Three unidentified 16S rRNA genus-specific amplicons of faecal origin were sequenced. Phylogenetic analysis showed that these sequences were 99% similar, and clustered within the genus as a novel group which was termed HS (HS = healthy subject). A PCR primer pair specific for the HS group was designed from the sequence data and used to reexamine the original samples. Although it was not possible to culture the organism from faeces, specific PCR assay detected it in 10 of the 20 faecal samples, but not in any corresponding saliva samples. The authors propose that the source of the amplicons is a previously undescribed and so far uncultivated species, which they term Campylobacter hominis’.

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Keyword(s): 16S rRNA sequences , Campylobacter and human gastrointestinal tract
© Society for General Microbiology 1998
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1998-08-01
2024-04-20
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References

  1. Anderson B.E., Dawson J.E., Jones D.C., Wilson K.H. 1991; Ehrlichia chaffensis, a new species associated with human ehrlichiosis.. J Clin Microbiol 29:2838–2842
     [Google Scholar]
  2. Anderson L.P., Norgaard A., Holck S., Blom J., Elsborg L. 1996; Isolation of a ‘Helicobacter heilmanii’-like organism from the human stomach.. Eur J Microbiol Infect Dis 15:95–96
     [Google Scholar]
  3. Bastyns K., Chapelle S., Vandamme P., Goossens H., De Wachter R. 1994; Species-specific detection of Campylobacters important in veterinary medicine by PCR amplification of 23S rDNA areas.. Syst Appl Microbiol 17:563–568
     [Google Scholar]
  4. Bastyns K., Chapelle S., Vandamme P., Goossens H., DeWachter R. 1995; Specific detection of Campylobacter concisus by PCR amplification of 23S rDNA areas.. Mol Cell Probes 9:247–250
     [Google Scholar]
  5. Berg R.D. 1996; The indigenous gastrointestinal microflora.. Trends Microbiol 4:430–435
     [Google Scholar]
  6. Boom R., Sol C.J.A., Salimans M.M.M., Jansen C.L, Wertheim van Dillen P.M.E., van der Noordaa J. 1990; Rapid and simple method for purification of nucleic acids.. J Clin Microbiol 28:495–503
     [Google Scholar]
  7. Corpet F. 1988; Multiple sequence alignment with hierarchical clustering.. Nucleic Acids Res 16:10881–10890
     [Google Scholar]
  8. Embley T.M. 1991; The linear PCR reaction: a simple and robust method for sequencing amplified rRNA genes.. Lett Appl Microbiol 13:171–174
     [Google Scholar]
  9. Etoh Y., Dewhirst F.E., Paster B.J., Yamamoto A., Goto N. 1993; Campylobacter showae sp. nov., isolated from the human oral cavity.. Int J Syst Bacteriol 43:631–639
     [Google Scholar]
  10. Goossens H., Butzler J.P. 1992; Isolation and identification of Campylobacter spp.. In Campylobacter jejuni: Current Status and Future Trends pp. 93–109 Edited by Nachamkin I., Blaser M.J., Tompkins L.S. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  11. Haddad A., Camacho F., Durand P., Cary S.C. 1995; Phylogenetic characterisation of the epibiotic bacteria associated with the hydrothermal vent polychaete Alvinella pompejana.. Appl Environ Microbiol 61:1679–1687
     [Google Scholar]
  12. Jones D.M., Sutcliffe E.M., Curry A. 1991; Recovery of viable but non-culturable Campylobacter jejuni.. J Gen Microbiol 137:2477–2482
     [Google Scholar]
  13. Jukes T.H., Cantor C.R. 1969; Evolution of protein molecules.. In Mammalian Protein Metabolism pp. 21–132 Edited by Munro H.N. New York: Academic Press;
     [Google Scholar]
  14. Lane D.J., Harrison A.P., Stahl D., Pace B., Giovannoni S.J., Olsen G.J., Pace N.R. 1992; Evolutionary relationships among sulfur- and iron-oxidizing eubacteria.. J Bacteriol 174:269–278
     [Google Scholar]
  15. Lawson A.J., Linton D., Stanley J., Owen R.J. 1997; Polymerase chain reaction detection and speciation of Campylobacter upsaliensis and C. helveticus in human faeces and comparison with culture techniques.. J Appl Microbiol 83:375–380
     [Google Scholar]
  16. Linton D., Owen R.J., Stanley J. 1996; Rapid identification by PCR of the genus Campylobacter and of five Campylobacter species enteropathogenic for man and animals.. Res Microbiol 147:707–718
     [Google Scholar]
  17. Linton D., Lawson A.J., Owen R.J., Stanley J. 1997; PCR detection, identification to species level and fingerprinting of Campylobacter jejuni and Campylobacter coli direct from diarrheic samples.. J Clin Microbiol 35:2568–2572
     [Google Scholar]
  18. Maidak B.L., Olsen G.L., Larsen N., Overbeek R., McGaughey M.J., Woese C.R. 1997; The RDP (Ribosomal Database Project).. Nucleic Acids Res 25:109–111
     [Google Scholar]
  19. Mortimer P.P., Parry J.V. 1994; Detection of antibody to HIV in saliva: a brief review.. Clin Diagn Virol 2:231–242
     [Google Scholar]
  20. Murray R.G.E., Schleifer K.H. 1994; Taxonomic notes: a proposal for recording the properties of putative taxa of procaryotes.. Int J Syst Bacteriol 44:174–176
     [Google Scholar]
  21. Murray R.G.E., Stackebrandt E. 1995; Taxonomic note: implementation of the provisional status Candidatus for incompletely described procaryotes.. Int J Syst Bacteriol 45:186–187
     [Google Scholar]
  22. Paster B.J., Dewhirst F.E. 1988; Phylogeny of Campylo-bacters, wolinellas, Bacteroides gracilis and Bacteroides ureoly- ticus by 16S ribosomal ribonucleic acid sequencing.. Int J Syst Bacteriol 38:56–62
     [Google Scholar]
  23. Rainey F.A., Toalster R., Stackebrandt E. 1993; Desufurella acetivorans, a thermophilic, acetate-oxidizing and sulfur-reducing organism, represents a distinct lineage within the Proteo-bacteria.. Syst Appl Microbiol 16:373–379
     [Google Scholar]
  24. Reiman D.A., Schmidt T.M., MacDermott R.P., Falkow S. 1992; Identification of the uncultured bacillus of Whipple’s disease.. N Engl J Med 327:293–301
     [Google Scholar]
  25. Saitou N., Nei M. 1987; The neighbour-joining method: a new method for reconstructing phylogenetic trees.. Mol Biol Evol 4:406–425
     [Google Scholar]
  26. Schoedon G., Goldenberger D., Forrer R., Gunz A., Dutly F., Hochli M., Altwegg M., Schaffner A. 1997; Deactivation of macrophages with IL-4 is the key to isolation of Tropheryma whippelii.. J Infect Dis 176:672–677
     [Google Scholar]
  27. Sebald M., Veron M. 1963; Teneur en bases de l’ADN et classification des vibrions.. Ann Inst Pasteur 105:897–910
     [Google Scholar]
  28. Skirrow M.B. 1994; Diseases due to Campylobacter, Helicobacter and related bacteria.. J Comp Pathol 111:113–149
     [Google Scholar]
  29. Solnick J.V., O’Rourke J., Lee A., Paster B.J., Dewhirst F.E., Tompkins L.S. 1993; An uncultured gastric spiral organism is a newly identified Helicobacter in humans.. J Infect Dis 168:379–385
     [Google Scholar]
  30. Steele T.W., McDermott S.N. 1984; The use of membrane filters applied directly to the surface of agar plates for the isolation of Campylobacter jejuni from faeces.. Pathology 16:263–265
     [Google Scholar]
  31. Tanner A.C.R., Badger S., Lai C.H., Listgarten M.A., Visconti R.A., Socransky S.S. 1981; Wolinella gen. nov., Wolinella succinogenes (Vibrio succinogenes Wolin et al.) comb, nov., and description of Bacteroides gracilis sp. nov., Wolinella recta sp. nov., Campylobacter concisus sp. nov., and Eikenella corrodens from humans with periodontal disease.. Int J Syst Bacteriol 31:432–435
     [Google Scholar]
  32. Tauxe R.V. 1992; Epidemiology of Campylobacter jejuni infections in the United States and other industrialized nations.. In Campylobacter jejuni: Current Status and Future Trends pp. 9–19 Edited by Nachamkin I., Blaser M. J., Tompkins L. S. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  33. Thompson L.M. III Smibert R. M., Johnson J.L., Krieg N.R. 1988; Phyogenetic study of the genus Campylobacter.. Int J Syst Bacteriol 38:190–200
     [Google Scholar]
  34. Vandamme P., Falsen E., Rossau R., Hoste B., Segers P., Tytgat R., De Ley J. 1991; Revision of Campylobacter, Helicobacter, and Wolinella taxonomy: emendation of generic descriptions and proposal of Arcobacter gen. nov.. Int J Syst Bacteriol 41:88–103
     [Google Scholar]
  35. Van de Peer Y., De Wachter R. 1993; treecon: a software package for the constuction and drawing of evolutionary trees.. Comput Appl Biosci 9:177–182
     [Google Scholar]
  36. Wilson M.J., Weightman A.J., Wade W.G. 1997; Applications of molecular ecology in the characterization of uncultured microorganisms associated with human disease.. Rev Med Microbiol 8:91–101
     [Google Scholar]
  37. Woese C.R. 1987; Bacterial evolution.. Microbiol Rev 51:221–271
     [Google Scholar]
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16S rRNA gene sequences of ‘Candidatus Campylobacter hominis’, a novel uncultivated species, are found in the gastrointestinal tract of healthy humans
Microbiology 144, 2063 (1998); https://doi.org/10.1099/00221287-144-8-2063
/content/journal/micro/10.1099/00221287-144-8-2063
/content/journal/micro/10.1099/00221287-144-8-2063
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