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Volume 144, Issue 8

Excess production of phage λ delayed early proteins under conditions supporting high Escherichia coli growth rates Free

Abstract

Bacteriophage λ is unable to lysogenize hosts harbouring the mutation due to a drastic reduction in transcription from CII-activated lysogenic promoters ( , and ). In addition, the level of early transcripts involved in the lytic pathway of λ development is also decreased in this genetic background due to impaired N-dependent antitermination. Here, it is demonstrated that despite the reduced level of early lytic - and -derived transcripts, lytic growth of bacteriophage λ is not affected in rich media. The level of the late lytic, -derived transcripts also remains unaffected by the mutation under these conditions. However, it was found that whilst there is no significant difference in the phage burst size in and hosts growing in rich media, phage λ is not able to produce progeny in the mutant growing in minimal medium, in contrast to otherwise isogenic bacteria. Provision of an excess of the phage replication proteins O and P or overproduction of the antitermination protein N restore the ability of phage λ to produce progeny in the mutant under the latter conditions. These results suggest that in rich media phage λ produces some early proteins in excess of that needed for its effective propagation and indicate that replication proteins may be limiting factors for phage lytic growth in poor media.

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Keyword(s): bacterial growth rate , bacteriophage λ development , Escherichia coli rpoA341 mutation , phage λ gene expression and λ replication proteins
© Society for General Microbiology 1998
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1998-08-01
2024-04-24
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Excess production of phage λ delayed early proteins under conditions supporting high Escherichia coli growth rates
Microbiology 144, 2217 (1998); https://doi.org/10.1099/00221287-144-8-2217
/content/journal/micro/10.1099/00221287-144-8-2217
/content/journal/micro/10.1099/00221287-144-8-2217
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