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Volume 145, Issue 10

detection of type 1 fimbrial expression and phase variation during experimental urinary tract infection Free

Abstract

Adhesion mediated by fimbriae is thought to play an important role in the pathogenesis of urinary tract infections (UTI) by . The majority of clinical isolates of from UTI are able to express type 1 fimbriae. However, the importance of these fimbriae as a virulence factor has been controversial. To investigate the expression of type 1 fimbriae during UTI, mice were transurethrally infected with uropathogenic C175-94 and type 1 fimbrial expression was determined directly by two independent methods at 2 h, 1 d and 3 d after infection. By use of an assay combining rRNA hybridization and immunofluorescence, all bacterial cells detected in urine, bladders and kidneys from mice sacrificed 1 and 3 d after onset of infection were found to express type 1 fimbriae. In contrast, the majority of cells in the suspension used for infection of mice and specimens from mice sacrificed 2 h after inoculation were found to be non-fimbriated. Similar results were obtained with a PCR assay revealing the orientation of the invertible promoter driving the transcription of type 1 fimbrial genes. Whilst the promoter in both ON and OFF positions could be amplified from the suspension used for infection and specimens from mice sacrificed 2 h after inoculation, at 1 and 3 d after onset of infection only the promoter in the ON orientation could be amplified. These results show that introduction of C175-94 into the mouse urinary tract leads to markedly enhanced expression of type 1 fimbriae.

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Keyword(s): immunofluorescence , in situ hybridization , phase variation , type 1 fimbriae , urinary tract infection and UTI, urinary tract infections
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1999-10-01
2024-04-18
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In vivo detection of Escherichia coli type 1 fimbrial expression and phase variation during experimental urinary tract infection
Microbiology 145, 2683 (1999); https://doi.org/10.1099/00221287-145-10-2683
/content/journal/micro/10.1099/00221287-145-10-2683
/content/journal/micro/10.1099/00221287-145-10-2683
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