1887

Microbiology Society logo

Volume 145, Issue 10

Galactomannans from the cell walls of species of sect. and their teleomorphs as immunotaxonomic markers Free

Abstract

An alkali-extractable and water-soluble fraction (F1S) was obtained from cell walls of and species of the related genera , and . The structure of the main polysaccharide of these fractions was studied and found to consist of a core of (1→6)-α-mannopyranose partially substituted at O-2 by chains of galactofuranose and shorter chains of mannopyranose. The differences in the regularity of the branching points and the length of the galactofuranose side chains are useful to distinguish between species. These differences were detected by immunological methods, since highly specific polyclonal antibodies were raised against these polysaccharides. Mycelium of CBS 990.73A was stained by indirect immunofluorescence. The polysaccharides studied in this work differ from the one described for species from section , and this is another indication of the heterogeneity of the genus .

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): cell wall , chemotaxonomy , fungal antibodies , galactomannan and Paecilomyces
© Microbiology Society
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-145-10-2789
1999-10-01
2024-05-21
Loading full text...

Full text loading...

/deliver/fulltext/micro/145/10/1452789a.html?itemId=/content/journal/micro/10.1099/00221287-145-10-2789&mimeType=html&fmt=ahah

References

  1. Adams, G. A. (1965). Complete acid hydrolysis. Methods Carbohydr Chem 5, 269-276. [Google Scholar]
  2. Aoki, J. & Yanase, K. (1970). Phenol oxidase activity in the integument of the silk-worm Bombyx mori infected with Beauvearia bassiana and Spicaria fumoso-rosea. J Invertebr Pathol 16, 459-464.[CrossRef] [Google Scholar]
  3. Bartnicki-Garcı́a, S. (1970). Cell wall composition and other biochemical markers in fungal phylogeny. In Phytochemical Phylogeny, pp. 81-103. Edited by J. B. Harborne. New York & London: Academic Press.
  4. Bartnicki-Garcı́a, S. (1987). The cell wall: a crucial structure in fungal evolution. In Evolutionary Biology of the Fungi, pp. 389-403. Edited by A. D. M. Rayner, C. M. Brasier & D. Moore. Cambridge: Cambridge University Press.
  5. Berbee, M. L., Yoshimura, A., Sugiyama, J. & Taylor, J. W. (1995). Is Penicillium monophyletic? An evaluation of phylogeny in the family Trichocomaceae from 18S, 5·8S and ITS ribosomal DNA sequence data. Mycologia 87, 210-222.[CrossRef] [Google Scholar]
  6. Brown, A. H. & Smith, G. (1957). The genus Paecilomyces Bainier and its perfect stage Byssochlamys Westling. Trans Br Mycol Soc 40, 17-89.[CrossRef] [Google Scholar]
  7. Byrd, R. P.Jr, Roy, T. M., Fields, C. L. & Lynch, J. A. (1992).Paecilomyces variotii pneumonia in a patient with diabetes mellitus. J Diabetes Complicat 6, 150-153.[CrossRef] [Google Scholar]
  8. Cohen, R. E. & Ballou, C. E. (1980). Linkage analysis of mannose-rich glycoprotein core oligosaccharides by proton nuclear magnetic resonance spectroscopy. Biochemistry 19, 4345-4358.[CrossRef] [Google Scholar]
  9. Currah, R. S. (1985). Taxonomy of the Onygenales: Athrodermataceae, Gymnoascaceae, Myxotrichaceae and Onygenaceae. Mycotaxon 24, 1-216. [Google Scholar]
  10. De Ruiter, G. A., Van Bruggen-van der Lugt, A. W., Nout, M. J. R., Middlehoven, W. J., Soentoro, P. S. S., Notermans, S. H. W. & Rombouts, F. M. (1992). Formation of antigenic extracellular polysaccharides by selected strains of Mucor spp., Rhizopus spp., Rhizomucor spp., Absidia corymbifera and Syncephalastrum racemosum. Antonie Leeuwenhoek 62, 189-199.[CrossRef] [Google Scholar]
  11. Domenech, J., Prieto, A., Bernabé, M. & Leal, J. A. (1994). Cell wall polysaccharides of four strains of P. variotii. Curr Microbiol 28, 169-173.[CrossRef] [Google Scholar]
  12. Domenech, J., Barasoaı́n, I., Prieto, A., Gómez-Miranda, B., Bernabé, M. & Leal, J. A. (1996). An antigenic water-soluble glucogalactomannan extracted from cell walls of Paecilomyces fumosoroseus and Paecilomyces farinosus. Microbiology 42, 3497-3503. [Google Scholar]
  13. Dubois, M., Gilles, K. A., Rebers, P. A. & Smith, F. A. (1956). Colorimetric method for determination of sugars and related substances. Anal Chem 28, 350-356.[CrossRef] [Google Scholar]
  14. Engel, G. & Teuber, M. (1991). Heat resistance of ascospores of Byssochlamys nivea in milk and cream. Int J Food Microbiol 12, 225-233.[CrossRef] [Google Scholar]
  15. Fennell, D. I. (1973). Plectomycetes: Eurotiales. In The Fungi, pp. 45-68. Edited by C. Ainsworth & A. S. Sussman. New York & London: Academic Press.
  16. Gailliez, J. C. & Poulain, D. (1988). Analyse cytologique de l’expression d’un épitope porté par les glycoprotéins excretées par Candida albicans. Ann Inst PasteurMicrobiol 139, 171-188. [Google Scholar]
  17. Gerwig, G. J., Kamerling, J. P. & Vliegenthart, J. F. (1978). Determination of the d and L configuration of neutral monosaccharides by high-resolution capillary G. L. C. Carbohydr Res 62, 349-357.[CrossRef] [Google Scholar]
  18. Gómez-Miranda, B., Rupérez, P. & Leal, J. A. (1981). Changes in chemical composition during germination of Botrytis cinerea sclerotia. Curr Microbiol 6, 243-246.[CrossRef] [Google Scholar]
  19. Gómez-Miranda, B., Prieto, A. & Leal, J. A. (1990). Chemical composition and characterization of a galactomannoglucan from Gliocladium viride wall material. FEMS Microbiol Lett 70, 331-336. [Google Scholar]
  20. Gorin, P. A. & Spencer, J. F. T. (1970). Proton magnetic resonance spectroscopy – an aid in identification and chemotaxonomy of yeasts. Adv Appl Microbiol 13, 25-89. [Google Scholar]
  21. Gray, G. R. (1987). Reductive cleavage of permethylated polysaccharides. Methods Enzymol 138, 26-38. [Google Scholar]
  22. Hearn, V. M. (1991). Glycoproteins of Aspergillus fumigatus cell wall. In Fungal Cell Wall and Immune Response, NATO ASI Series H, Cell Biology, pp. 219-228. Edited by J. P. Latgé & D. Boucias. Berlin: Springer.
  23. Jiménez-Barbero, J., Bernabé, M., Leal, J. A., Prieto, A. & Gómez-Miranda, B. (1993). Chemical structure and conformational features of cell-wall polysaccharides isolated from Aphanoascus mephitalus and related species. Carbohydr Res 250, 289-299.[CrossRef] [Google Scholar]
  24. Jiménez-Barbero, J., Prieto, A., Gómez-Miranda, B., Leal, J. A. & Bernabé, M. (1995). Chemical structure of fungal cell-wall polysaccharides isolated from Microsporum gypseum and related species of Microsporum and Trychophyton. Carbohydr Res 272, 121-128.[CrossRef] [Google Scholar]
  25. Kamphuis, H. J., De Ruiter, G. A., Veeneman, G. H., Van Boom, J. H., Rombouts, F. M. & Notermans, S. H. W. (1992). Detection of Aspergillus and Penicillium extracellular polysaccharides (EPS) by ELISA: using antibodies raised against acid hydrolysed EPS. Antonie Leeuwenhoek 61, 323-332.[CrossRef] [Google Scholar]
  26. King, D. A.Jr, Michener, H. D. & Ito, K. A. (1969). Control of Byssochlamys and related heat-resistant fungi in grape products. Appl Microbiol 18, 166-173. [Google Scholar]
  27. Leal, J. A. (1994). Water-soluble polysaccharides of fungal cell walls. In Microorganisms in Ruminant Nutrition, pp. 153-165. Edited by R. A. Prins & C. S. Stewart. Nottingham: Nottingham University Press.
  28. Leal, J. A. & Bernabé, M. (1998). Taxonomic applications of polysaccharides. In Chemical Fungal Taxonomy, pp. 153-181. Edited by J. C. Frisvad, P. D. Bridge & D. K. Arora. New York & Hong Kong: Marcel Dekker.
  29. Leal, J. A., Gómez-Miranda, B., Bernabé, M., Cano, J. & Guarro, J. (1992). The chemical composition of six species of Aphanoascus: the taxonomic significance of the presence of α-(1–2)-(1–6) mannan and α-(1–4) glucan. Mycol Res 96, 363-368.[CrossRef] [Google Scholar]
  30. Leal, J. A., Prieto, A., Gómez-Miranda, B., Jiménez-Barbero, J. & Bernabé, M. (1993). Structure and conformational features of an alkali- and water-soluble galactofuranan from the cell walls of Eupenicillium crustaceum. Carbohydr Res 244, 361-368.[CrossRef] [Google Scholar]
  31. Leal, J. A., Gómez-Miranda, B., Prieto, A., Domenech, J., Ahrazem, O. & Bernabé, M. (1997). Possible chemotypes from cell wall polysaccharides as an aid in the systematics of the genus Penicillium and its teleomorphic states Eupenicillium and Talaromyces. Mycol Res 101, 1259-1264.[CrossRef] [Google Scholar]
  32. Notermans, S. & Soentoro, P. S. S. (1986). Immunological extracellular polysaccharide antigens produced by different mould species. Antonie Leeuwenhoek 52, 393-401.[CrossRef] [Google Scholar]
  33. Notermans, S., Wieten, G., Engel, H. W. B., Rombouts, F. M., Hoogerhout, P. & Van Boom, J. H. (1987). Purification of extracellular polysaccharides (EPS) antigens produced by different mould species. J Appl Bacteriol 62, 157-166.[CrossRef] [Google Scholar]
  34. Notermans, S., Dufrenne, J., Wijnands, L. M. & Engel, H. W. B. (1988). Human serum antibodies to extracellular polysaccharides (EPS) of moulds. Int J Food Microbiol 2, 247-258. [Google Scholar]
  35. Parra, E., Jiménez-Barbero, J., Bernabé, M., Leal, J. A., Prieto, A. & Gómez-Miranda, B. (1994). Structural investigation of two cell wall polysaccharides of Penicillium expansum strains. Carbohydr Res 257, 239-248.[CrossRef] [Google Scholar]
  36. Pitt, J. I. (1993). Speciation and evolution in Penicillium and related genera. In The Fungal Holomorph: Mitotic, Meiotic and Pleomorphic Speciation in Fungal Systematics, pp. 107-113. Edited by D. R. Reynolds & J. W. Taylor. Wallingford: CAB International.
  37. Prieto, A., Bernabé, M. & Leal, J. A. (1995). Isolation, purification and chemical characterization of alkali-extractable polysaccharides from the cell walls of Talaromyces species. Mycol Res 99, 69-75.[CrossRef] [Google Scholar]
  38. Prieto, A., Leal, J. A., Poveda, A., Jiménez-Barbero, J., Gómez-Miranda, B., Domenech, J., Ahrazem, O. & Bernabé, M. (1997). Structure of complex cell wall polysaccharides isolated from Trichoderma and Hypocrea species. Carbohydr Res 304, 281-291.[CrossRef] [Google Scholar]
  39. Rath, J., Messner, R., Kosma, P., Altmann, F., März, L. & Kubicek, C. P. (1995). The α-d-mannan core of a complex cell-wall heteroglycan of Trichoderma reesei is responsible for β-glucosidase activation. Arch Microbiol 164, 414-419. [Google Scholar]
  40. Samson, R. A. (1974).Paecilomyces and some allied hyphomycetes. Stud Mycol 6, 1-119. [Google Scholar]
  41. Sridhara, S., Gandal, S. V. & Joshi, A. P. (1990). Immunochemical investigation of allergens from Rhizopus nigricans. Allergy 45, 577-586.[CrossRef] [Google Scholar]
  42. Taylor, J. W., Pitt, J. I. & Hocking, A. D. (1990). Ribosomal DNA restriction studies of Talaromyces species with Paecilomyces and Penicillium anamorphs. In Modern Concepts in Penicillium and Aspergillus Classification, pp. 357-370. Edited by R. A. Samson & J. I. Pitt. New York: Plenum.
  43. Tigano-Milani, M. S., Samson, R. A., Martins, I. & Sobral, B. W. S. (1995). DNA markers for differentiating isolates of Paecilomyces lilacinus. Microbiology 141, 239-245.[CrossRef] [Google Scholar]
  44. Vliegenthart, J. F. G., Dorland, L. & van Halbeck, H. (1983). High-resolution 1H-NMR spectroscopy as a tool in the structural analysis of carbohydrates related to glycoproteins. Adv Carbohydr Chem Biochem 41, 209-374. [Google Scholar]
  45. Weijman, A. C. M. & Golubev, W. I. (1987). Carbohydrate patterns and taxonomy of yeasts and yeast-like fungi. In The Expanding Realm of Yeast-like Fungi, pp. 361-371. Edited by G. S. de Hoog, M. T. H. Smith & A. C. M. Weijman. Amsterdam: Elsevier.
  46. Weijman, A. C. M. & Van der Walt, J. P. (1989). Carbohydrate profiles and the taxonomic concept in the Lipomycetaceae. Stud Mycol 31, 193-199. [Google Scholar]
  47. Weijman, A. C. M., Vlug, I. J. A. & Van Eijk, G. W. (1982). Carbohydrate patterns. Stud Mycol 22, 50-53. [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-145-10-2789
Loading
Galactomannans from the cell walls of species of Paecilomyces sect. Paecilomyces and their teleomorphs as immunotaxonomic markers
Microbiology 145, 2789 (1999); https://doi.org/10.1099/00221287-145-10-2789
/content/journal/micro/10.1099/00221287-145-10-2789
/content/journal/micro/10.1099/00221287-145-10-2789
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error