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Volume 145, Issue 12

The Hsp90 of can confer Hsp90 functions in : a potential model for the processes that generate immunogenic fragments of this molecular chaperone in infections Free

Abstract

During infections with a number of important eukaryotic pathogens the Hsp90 molecular chaperone of the pathogen is recognized as an immunodominant antigen by the host immune system. Yeast molecular genetics should allow study of the extent of sequence variation within conserved immunodominant epitopes on pathogen Hsp90s that is compatible with essential Hsp90 functions, as well as the processes that generate antigenic subfragments of these Hsp90s. The Hsp90 of the fungal pathogen was shown in this study to provide both essential and nonessential (pheromone signalling and mammalian steroid receptor activation) Hsp90 functions in cells. Much of the Hsp90 expressed in respiratory cells was shown to undergo a partial degradation , a degradation that closely resembles that of the native Hsp82 (one isoform of the homologous Hsp90) in . Allowing for the differences in the length of the charged linker region between the N- and C-terminal domains of Hsp90 and Hsp82, these two proteins expressed in appear to give the same major degradation products. These Hsp90 fragments are similar to the products of incomplete Hsp90 degradation found in cultures.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): candidiasis , 5-FOA, 5-fluoroorotic acid , Candida albicans , GR, glucocorticoid receptor , Hsp90 , partial protein degradation and Saccharomyces cerevisiae
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1999-12-01
2024-04-20
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The Hsp90 of Candida albicans can confer Hsp90 functions in Saccharomyces cerevisiae: a potential model for the processes that generate immunogenic fragments of this molecular chaperone in C. albicans infections
Microbiology 145, 3455 (1999); https://doi.org/10.1099/00221287-145-12-3455
/content/journal/micro/10.1099/00221287-145-12-3455
/content/journal/micro/10.1099/00221287-145-12-3455
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