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Volume 146, Issue 8

Expression of the phospho-β-glycosidase ArbZ from subsp. in : substrate induction and catabolite repression Free

Abstract

ArbZ from subsp. was previously shown to enable utilization of the β-glucoside arbutin by . The gene was cloned and expressed in the industrially used β-glucoside-negative strain 3036(62). The transformants were able to ferment not only arbutin, but also cellobiose, salicin and methyl-β-glucoside (MβGlc). Cleavage of β-glucosides by the transformants depended on the integrity of the cytoplasmic membrane, whereas in cell-free extracts only C-phosphorylated substrates were hydrolysed. This suggested that ArbZ is a phospho-β-glycosidase. ArbZ activity in transformants of was subject to substrate induction mediated by the β-glucosides arbutin, salicin and MβGlc, whereas cellobiose or the β-galactoside lactose had no inducing effect. Northern blot analysis proved that induction by MβGlc was due to enhanced transcription of . Catabolite repression of induction was observed with glucose, mannose, fructose and galactose. The induction kinetics observed in the presence of these sugars indicated that at least two different mechanisms are operative in catabolite repression of in .

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Keyword(s): ArbZ phospho-β-glycosidase , catabolite repression , Lactobacillus delbrueckii , MβGlc, methyl-β-glucoside , oNPGal, o-nitrophenyl β-D-galactopyranoside , oNPGalP, oNPGal 6-phosphate , pNPGlc, p-nitrophenyl β-D-glucopyranoside , PTS, phosphotransferase system and substrate induction
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2000-08-01
2024-04-26
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Expression of the phospho-β-glycosidase ArbZ from Lactobacillus delbrueckii subsp. lactis in Lactobacillus helveticus: substrate induction and catabolite repression
Microbiology 146, 1941 (2000); https://doi.org/10.1099/00221287-146-8-1941
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