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Volume 147, Issue 10

Adherence of to respiratory tract epithelial cells and inhibition with dextrans Free

Abstract

Adherence of to cells of the respiratory tract of patients with cystic fibrosis (CF) appears to be a necessary precondition for colonization and infection. To date, no effective anti-adhesive strategy has been devised for preventing infection in CF patients. It was found in this study that adhered to respiratory epithelial cells both and . However, strains with cable-like pili (Cbl) exhibited the typical clump formation on pneumocytes, whereas non-cable piliated strains predominantly showed single-cell adherence. Dextrans (nominally 4000–10000 Da) significantly inhibited adhesion of to A549 pneumocytes. When compared on an equal weight basis, the nominally 10000 Da dextran was most inhibitory. A dose-dependent inhibitory effect (up to 80 mg ml) was observed for most strains. Dextran exerted less of an anti-adhesive effect on the two Cbl strains than on the others which were Cbl. Dextrans appeared to block the adherence in a non-specific fashion, as shown by the observations that the inhibitory effect was readily reversible and oligosaccharides composed of 2–4 glucose units with the same α-1,6 linkage were not inhibitory. The mean molecular masses of dextrans used in this study, as determined by gel filtration and MS, were approximately 10-fold lower than those indicated by the manufacturers. Our data suggest that dextran of nominal molecular mass 4000 Da at a concentration of 40 mg ml (10 mM according to manufacturer’s quoted molecular mass) or more may be useful in patients with CF to prevent colonization and infection with .

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): CF, cystic fibrosis and cystic fibrosis
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2001-10-01
2024-04-25
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References

  1. Ashford D., Dwek P. A., Welply J. K., Amatayakul S., Homans S. W., Lis H., Taylor G. N., Sharon N., Rademacher T. W. 1987; The beta-2-d-xylose and alpha-1–3-l-fucose substituted N -linked oligosaccharides from Erythrina cristagalli lectin. Isolation, characterization and comparison with other legume lectins. Eur J Biochem 166:311–320 [CrossRef]
     [Google Scholar]
  2. Barghouthi S., Guerdoud L. M., Speert D. P. 1996; Inhibition by dextran of Pseudomonas aeruginosa adherence to epithelial cells. Am J Respir Crit Care Med 154:1788–1793 [CrossRef]
     [Google Scholar]
  3. Bigge J. C., Patel T. P., Bruce J. A., Goulding P. N., Charles S. M., Parekh R. B. 1995; Nonselective and efficient fluorescent labeling of glycans using 2-amino benzamide and anthranilic acid. Anal Biochem 230:229–238 [CrossRef]
     [Google Scholar]
  4. Bryan R., Feldman M., Jawetz S. C., Rajan S., DiMango E., Tang H. B., Scheffler L., Speert D. P., Prince A. 1999; The effects of aerosolized dextran in a mouse model of Pseudomonas aeruginosa pulmonary infection. J Infect Dis 179:1449–1458 [CrossRef]
     [Google Scholar]
  5. Burns J. L., Jonas M., Chi E. Y., Clark D. A., Berger A., Griffith A. 1996; Invasion of respiratory epithelial cells by Burkholderia ( Pseudomonas ) cepacia . Infect Immun 64:4054–4059
     [Google Scholar]
  6. Butler S. L., Nelson J. W., Poxton I. R., Govan J. R. W. 1994; Serum sensitivity of Burkholderia ( Pseudomonas ) cepacia isolates from patients with cystic fibrosis. FEMS Immunol Med Microbiol 8:285–292 [CrossRef]
     [Google Scholar]
  7. Canadian cystic fibrosis foundation medical/scientific advisory committee; 1993; Epidemiology of Pseudomonas cepacia in cystic fibrosis. Can J Infect Dis 4:163–164
     [Google Scholar]
  8. Cronberg S., Robertson B., Nilsson I. M., Nilehn J. E. 1966; Suppressive effect of dextran on platelet adhesiveness. Thromb Diath Haemorrh 16:384–394
     [Google Scholar]
  9. Feng W., Garrett H., Speert D. P., King M. 1996; Improved clearability of cystic fibrosis sputum with dextran treatment in vitro . Am J Respir Crit Care Med 154:1788–1793 [CrossRef]
     [Google Scholar]
  10. Finlay W. H., Lange C. F., King M., Speert D. P. 2000; Lung delivery of aerosolized dextran. Am J Respir Crit Care Med 16:91–97
     [Google Scholar]
  11. Goldstein R., Sun L., Jiang R. Z., Sajjan U. S., Forstner J. F., Campbell C. 1995; Structurally variant classes of pilus appendage fibers coexpressed from Burkholderia ( Pseudomonas ) cepacia . J Bacteriol 177:1039–1052
     [Google Scholar]
  12. Govan J. R., Hughes J. E., Vandamme P. 1996; Burkholderia cepacia : medical, taxonomic, and ecological issues. J Med Microbiol 45:395–407 [CrossRef]
     [Google Scholar]
  13. Guile G. R., Rudd P. M., Wing D. R., Prime S. B., Dwek R. A. 1996; A rapid high-resolution high-performance liquid chromatographic method for separating glycan mixtures and analyzing oligosaccharide profiles. Ann Biochem 240:210–226 [CrossRef]
     [Google Scholar]
  14. Isles A., MacLusky I., Corey M., Gold R., Prober C., Fleming P., Levison H. 1984; Pseudomonas cepacia infection in cystic fibrosis: an emerging problem. J Pediatr 104:206–210 [CrossRef]
     [Google Scholar]
  15. Kuehn M., Lent K., Haas J., Hagenzicker J., Cervin M., Smith A. L. 1992; Fimbriation of Pseudomonas cepacia . Infect Immun 60:2002–2007
     [Google Scholar]
  16. Kuster B., Wheeler S. F., Hunter A. P., Dwek R. A., Harvey D. J. 1997; Sequencing of N -linked oligosaccharides directly from protein gels: in-gel deglycosylation followed by matrix-assisted laser desorption/ionization mass spectrometry and normal-phase high-performance liquid chromatography. Ann Biochem 250:82–101 [CrossRef]
     [Google Scholar]
  17. Mahenthiralingam E., Campbell M. E., Henry D. A., Speert D. P. 1996; Epidemiology of Burkholderia cepacia infection in patients with cystic fibrosis: analysis by randomly amplified polymorphic DNA fingerprinting. J Clin Microbiol 34:2914–2920
     [Google Scholar]
  18. Mahenthiralingam E., Simpson D. A., Speert D. P. 1997; Identification and characterization of a novel DNA marker associated with epidemic Burkholderia cepacia strains recovered from patients with cystic fibrosis. J Clin Microbiol 35:808–816
     [Google Scholar]
  19. Saini L. S., Galsworthy S. B., John M. A., Valvano M. A. 1999; Intracellular survival of Burkholderia cepacia complex isolates in the presence of macrophage cell activation. Microbiology 145:3465–3475
     [Google Scholar]
  20. Sajjan U. S., Forstner J. F. 1992; Identification of the mucin-binding adhesin of Pseudomonas cepacia isolates from patients with cystic fibrosis. Infect Immun 60:1434–1440
     [Google Scholar]
  21. Sajjan U. S., Forstner J. F. 1993; Role of the 22-kilodalton pilin protein in binding of Pseudomonas cepacia to buccal epithelial cells. Infect Immun 61:3157–3163
     [Google Scholar]
  22. Sajjan U. S., Sun L., Goldstein R., Forstner J. F. 1995; Cable (Cbl) type II pili of cystic fibrosis-associated Burkholderia ( Pseudomonas ) cepacia : nucleotide sequence of the cblA major subunit pilin gene and novel morphology of the assembled appendage fibers. J Bacteriol 177:1030–1038
     [Google Scholar]
  23. Sajjan U. S., Sylvester F. A., Forstner J. F. 2000; Cable-piliated Burkholderia cepacia binds to cytokeratin 13 of epithelial cells. Infect Immun 68:1787–1795 [CrossRef]
     [Google Scholar]
  24. Snell G. I., de Hoyos A., Krajden M., Winton T., Maurer J. R. 1993; Pseudomonas cepacia in lung transplant recipients with cystic fibrosis. Chest 103:466–471 [CrossRef]
     [Google Scholar]
  25. Speert D. P. 1989; Prevention of severe lower respiratory infections in patients with cystic fibrosis. Semin Respir Infect 4:266–271
     [Google Scholar]
  26. Tablan O. C., Chobra T. L., Schidlow D. V. 7 other authors 1985; Pseudomonas cepacia colonization in patients with cystic fibrosis: risk factors and clinical outcome. J Pediatr 107:382–387 [CrossRef]
     [Google Scholar]
  27. Vandamme P., Holmes B., Vancanneyt M. 8 other authors 1997; Occurrence of multiple genomovars of Burkholderia cepacia in cystic fibrosis patients and proposal of Burkholderia multivorans sp. nov. Int J Syst Bacteriol 47:1188–1200 [CrossRef]
     [Google Scholar]
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Adherence of Burkholderia cepacia to respiratory tract epithelial cells and inhibition with dextrans
Microbiology 147, 2651 (2001); https://doi.org/10.1099/00221287-147-10-2651
/content/journal/micro/10.1099/00221287-147-10-2651
/content/journal/micro/10.1099/00221287-147-10-2651
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