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Volume 148, Issue 6

Role of the SspB protein in the development of biofilms on streptococcal substrates Free

Abstract

is an aggressive periodontal pathogen that persists in the mixed-species plaque biofilm on tooth surfaces. cells attach to the plaque commensal and this coadhesion event leads to the development of biofilms. Binding of these organisms is multimodal, involving both the major fimbrial FimA protein and the species-specific interaction of the minor fimbrial Mfa1 protein with the streptococcal SspB protein. This study examined the contribution of the Mfa1–SspB interaction to biofilm formation. biofilms readily formed on substrata of DL1 but not on cells which lack a coadhesion-mediating homologue of SspB. An insertional inactivation of the gene in resulted in a phenotype deficient in binding and unable to form biofilms. Furthermore, analysis using recombinant streptococci and enterococci showed that biofilms formed on strains expressing SspB or translational fusions of SspB with SpaP (the non-adherent SspB homologue in ) containing the adherence domain (Ssp dherence egion, BAR) of SspB. In contrast, an isogenic Ssp null mutant of DL1 was unable to support biofilm growth, even though this strain bound to FimA at levels similar to wild-type DL1. Finally, site-specific mutation of two functional amino acid residues in BAR resulted in SspB polypeptides that did not promote the development of biofilms. These results suggest that the induction of biofilms on a streptococcal substrate requires functional SspB–minor fimbriae interactions.

  • Received:
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Keyword(s): antigen I/II , BAR, SspB adherence region , coadhesion , CSLM, confocal scanning laser microscopy and minor fimbriae
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2002-06-01
2024-04-16
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Role of the Streptococcus gordonii SspB protein in the development of Porphyromonas gingivalis biofilms on streptococcal substrates
Microbiology 148, 1627 (2002); https://doi.org/10.1099/00221287-148-6-1627
/content/journal/micro/10.1099/00221287-148-6-1627
/content/journal/micro/10.1099/00221287-148-6-1627
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