%0 Journal Article %A Hanson, K. G. %A Steinhauer, Katrin %A Reizer, Jonathan %A Hillen, Wolfgang %A Stülke, Jörg %T HPr kinase/phosphatase of Bacillus subtilis: expression of the gene and effects of mutations on enzyme activity, growth and carbon catabolite repression %D 2002 %J Microbiology, %V 148 %N 6 %P 1805-1811 %@ 1465-2080 %R https://doi.org/10.1099/00221287-148-6-1805 %K FBP, fructose 1,6-bisphosphate %K Walker A box %K PTS, phosphotransferase system %K mutagenesis %K glycolysis %K HPrK/P, HPr kinase/phosphatase %K phosphorylation %K CCR, carbon catabolite repression %I Microbiology Society, %X HPr kinase/phosphatase (HPrK/P) is the key protein in regulation of carbon metabolism in Bacillus subtilis and many other Gram-positive bacteria. Whether this enzyme acts as a kinase or phosphatase is determined by the nutrient status of the cell. Mutational analysis of residues in a Walker A box nucleotide-binding motif revealed that it is not only important for kinase but is also involved in phosphatase activity. In addition, a signature sequence specifically conserved among HPrK/P orthologues is required for phosphatase activity and may be involved in interaction with HPr/HPr-(Ser46)-P. Carbon catabolite repression was abolished in a B. subtilis strain expressing a mutant form of HPrK/P deficient in kinase and phosphatase activities. The growth characteristics of this strain were similar to those of the wild-type. In contrast, B. subtilis strains expressing HPrK/P with partial kinase and no phosphatase activities showed growth impairment but exhibited catabolite repression. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-148-6-1805