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Volume 148, Issue 9

Activation of the transcription factor NF-κB by Free

Abstract

is a food-borne pathogen responsible for infectious enterocolitis. The early-response transcription factor NF-κB triggers the expression of genes associated with cellular immune and inflammatory responses. Co-incubation of HeLa cells with viable leads to the activation of the transcription factor NF-κB as determined by specific induction of a cellular luciferase-based reporter. Boiled cell-free extracts of are also potent dose-dependent stimulators of NF-κB-dependent transcription, the levels of which can reach up to 1000-fold as compared with independent controls. Using both cultured HeLa cells and human colonic epithelial (HCA-7) cells, the activation of NF-κB by boiled extract has been monitored through the degradation of IKBα and DNA binding of the nuclear translocated p50/p65 heterodimer of NF-κB. These events are co-ordinated with elaboration of the pro-inflammatory cytokine interleukin-8. Fractionation of the boiled extract suggests that the majority of the bioactive component has a molecular mass of 3 kDa or less, which is insensitive to proteinase K treatment.

  • Received:
  • Accepted:
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  • Published Online:
Keyword(s): BCE, boiled-cell extract , EMS, electrophoretic mobility shift , food safety , IL, interleukin , inflammation , innate immunity , interleukin-8 , LOS, lipo-oligosaccharide , TNF-α , transcription and tumour necrosis factor-α
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2002-09-01
2024-04-18
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Activation of the transcription factor NF-κB by Campylobacter jejuni
Microbiology 148, 2753 (2002); https://doi.org/10.1099/00221287-148-9-2753
/content/journal/micro/10.1099/00221287-148-9-2753
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