Activation of the transcription factor NF-κB by Campylobacter jejuni
Mellits, Kenneth H. and Mullen, Joseph and Wand, Matthew and Armbruster, Gisèle and Patel, Amit and Connerton, Phillippa L. and Skelly, Maeve and Connerton, Ian F.,, 148, 2753-2763 (2002),
doi = https://doi.org/10.1099/00221287-148-9-2753,
publicationName = Microbiology Society,
issn = 1350-0872,
abstract= Campylobacter jejuni is a food-borne pathogen responsible for infectious enterocolitis. The early-response transcription factor NF-κB triggers the expression of genes associated with cellular immune and inflammatory responses. Co-incubation of HeLa cells with viable C. jejuni leads to the activation of the transcription factor NF-κB as determined by specific induction of a cellular luciferase-based reporter. Boiled cell-free extracts of C. jejuni are also potent dose-dependent stimulators of NF-κB-dependent transcription, the levels of which can reach up to 1000-fold as compared with independent controls. Using both cultured HeLa cells and human colonic epithelial (HCA-7) cells, the activation of NF-κB by C. jejuni boiled extract has been monitored through the degradation of IKBα and DNA binding of the nuclear translocated p50/p65 heterodimer of NF-κB. These events are co-ordinated with elaboration of the pro-inflammatory cytokine interleukin-8. Fractionation of the boiled C. jejuni extract suggests that the majority of the bioactive component has a molecular mass of 3 kDa or less, which is insensitive to proteinase K treatment.,
language=,
type=