1887

Abstract

When a sensitive population of is exposed to streptomycin, two effects of the drug on viability can be experimentally differentiated: (1) a sublethal injury from which the cells recover once streptomycin is removed, and (2) a lethal injury from which no recovery occurs. Protein synthesis is markedly inhibited by streptomycin before sublethal or lethal effects on viability are noted. The effect on protein synthesis of exposure to streptomycin cannot be reversed by washing the cells by centrifugation, but protein synthesis recovers its original rate on further incubation after streptomycin is removed. It is postulated that streptomycin causes both sublethal and lethal injury to cells by irreversibly blocking protein synthesis, possibly by inactivating ribosomes.

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/content/journal/micro/10.1099/00221287-35-1-159
1964-04-01
2024-05-02
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References

  1. Erdös T., Ullmann A. 1959; Effect of streptomycin on the incorporation of amino acids labelled with C14 into RNA and protein in cell-free systems of a Mycobacterium.. Nature, Lond. 183:618
    [Google Scholar]
  2. Flaks J. G., Cox E. C., White J. R. 1962; Inhibition of polypeptide synthesis by streptomycin.. Biochem. Biophys. Res. Comm. 7:385
    [Google Scholar]
  3. Flaks J. G., Witting M. L. 1963; The mode of action of streptomycin.. Bact. Proc. 118:118
    [Google Scholar]
  4. Hurwitz C., Doppel H. W. 1963; Effect of streptomycin on Mycobacterium fortuitum.. Bact. Proc. 12:12
    [Google Scholar]
  5. Hurwitz C., Landau J. V., Doppel H. W. 1962; Effect of exposure of Escherichia coli to streptomycin on ability to undergo cell division.. J. Bact. 84:1116
    [Google Scholar]
  6. Hurwitz C., Rosano C. L. 1962; Accumulation of label from C14-streptomycin by Escherichia coli.. J. Bact. 83:1193
    [Google Scholar]
  7. Hurwitz C., Rosano C. L., Landau J. V. 1962; Kinetics of loss of viability of Escherichia coli exposed to streptomycin.. J. Bact. 83:1210
    [Google Scholar]
  8. Spotts C. R., Stanier R. Y. 1961; Mechanism of streptomycin action on bacteria: A unitary hypothesis.. Nature, Lond. 192:633
    [Google Scholar]
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