1887

Abstract

SUMMARY

From strain c, made F by infection with the sex factor F normally carried by strain -12, several Hfr (‘high frequency of recombination’) strains were derived. Among these, four were found which exhibited a defective growth pattern on minimal media at 37°. Reversion to the F state was accompanied by re-establishment of normal growth habit. In the case best studied (strain c-132) the Hfr bacteria form colonies smaller than normal, acquire a rough surface upon prolonged incubation, and are unable to grow at 42°. Growth is normal at room temperature and on rich media; it can be improved by the addition of methionine to minimal media. The rate of reversion from the Hfr to the F state (i.e. from defective to normal growth) is of the order of 1/20,000 per generation. Defective growth is not due to a genetic peculiarity of the F factor, nor is it dependent on the map location of the ‘ origin’ or leading end of the particular Hfr strain, or on its direction of chromosome transfer; possibly it results from the manner in which the F factor is integrated at any given chromosomal site.

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/content/journal/micro/10.1099/00221287-40-3-365
1965-09-01
2024-04-18
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References

  1. Bertani G. 1951; Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli . J. Bact 62:293
    [Google Scholar]
  2. Bertani G., Six E. 1958; Inheritance of P2 prophage in bacterial crosses. Virology 6:357
    [Google Scholar]
  3. Bertani G., Weigle J. J. 1953; Host controlled variation in bacterial viruses. J. Bact 65:113
    [Google Scholar]
  4. Campbell A. M. 1962; Episomes. Advanc. Genet 11:101
    [Google Scholar]
  5. Fisher K. W. 1957; The role of the Krebs cycle in conjugation in Escherichia coli k-12. J. gen. Microbiol 16:120
    [Google Scholar]
  6. Hayes W. 1964 The Genetics of Bacteria and Their Viruses Oxford: Blackwell;
    [Google Scholar]
  7. Jacob F., Wollman E. 1961 Sexuality and the Genetics of Bacteria New York and London: Academic Press;
    [Google Scholar]
  8. Kelly B. 1963; Localization of P 2 prophage in two strains of Escherichia coli . Virology 19:32
    [Google Scholar]
  9. Ledekberg J., Lederberg E. M. 1952; Replica plating and indirect selection of bacterial mutants. J. Bact 63:399
    [Google Scholar]
  10. Lieb M., Weigle J. J., Kellenberger E. 1955; A study of hybrids between two strains of Escherichia coli . J. Bact 69:468
    [Google Scholar]
  11. Luria S. E., Delbruck M. 1943; Mutations of bacteria from virus sensitivity to virus resistance. Genetics 28:491
    [Google Scholar]
  12. Nagata T. 1963; The sequential replication of E. coli DNA. Cold Spr. Harb. Symp. quant. Biol 2855
    [Google Scholar]
  13. Six E. 1959; The rate of spontaneous lysis of lysogenic bacteria. Virology 7:328
    [Google Scholar]
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