%0 Journal Article %A SASAKI, I. %A BERTANI, G. %T Growth abnormalities in Hfr Derivatives of Escherichia coli Strain c %D 1965 %J Microbiology, %V 40 %N 3 %P 365-376 %@ 1465-2080 %R https://doi.org/10.1099/00221287-40-3-365 %I Microbiology Society, %X SUMMARY From Escherichia coli strain c, made F+ by infection with the sex factor F normally carried by E. coli strain k-12, several Hfr (‘high frequency of recombination’) strains were derived. Among these, four were found which exhibited a defective growth pattern on minimal media at 37°. Reversion to the F+ state was accompanied by re-establishment of normal growth habit. In the case best studied (strain c-132) the Hfr bacteria form colonies smaller than normal, acquire a rough surface upon prolonged incubation, and are unable to grow at 42°. Growth is normal at room temperature and on rich media; it can be improved by the addition of methionine to minimal media. The rate of reversion from the Hfr to the F+ state (i.e. from defective to normal growth) is of the order of 1/20,000 per generation. Defective growth is not due to a genetic peculiarity of the F factor, nor is it dependent on the map location of the ‘ origin’ or leading end of the particular Hfr strain, or on its direction of chromosome transfer; possibly it results from the manner in which the F factor is integrated at any given chromosomal site. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-40-3-365