The Effect of Environmental Conditions on the Motility of Escherichia coli ADLER, J. and TEMPLETON, BONNIE,, 46, 175-184 (1967), doi = https://doi.org/10.1099/00221287-46-2-175, publicationName = Microbiology Society, issn = 1350-0872, abstract= SUMMARY A simple chemically defined medium for examining the motility of Escherichia coli k12 was designed. The essential components were: (1) a chelating agent to protect the motility against inhibition by traces of heavy metal ions; (2) a buffer to keep the pH value at the optimum between pH 6·0 and 7·5; (3) an energy source to stimulate the motility above that allowed by an endogenous energy source. Oxygen was required unless an energy source was provided which yielded energy anaerobically. A temperature optimum was determined. A chemically defined growth medium capable of producing motile bacteria was devised. It was found that the presence of glucose or growth above 37° prevented synthesis of flagella., language=, type=