1887

Abstract

SUMMARY

Thin sections of harvested during the logarithmic phase of growth in alkaline peptone water or in syncase medium have revealed an excretion process of the cell wall in the form of bulging out and pinching-off of portions. An identical phenomenon has also been revealed in cells harvested after a short period of incubation (1·5 hr) in glucose saline solution at 37°. Particles closely resembling the pinched-off cell-wall structures have been detected by metal shadowing and negative staining techniques in the bacteria-free filtrates of the log phase cultures (in both media) and the glucose saline incubation medium. These particles are in the range 400–1100 A in size with a maximum frequency in the range 600–800 A. No similar cell-wall changes have been detected in vibrios-harvested from the stationary phase of growth in any of the culture media, nor in vibrios undergoing plasmolysis. Turbidimetric tests revealed no significant lysis of the vibrios when harvested during the logarithmic growth phase and incubated for several hours in saline or in Kellenberger buffer as compared to the lysis detected in distilled water. It is suggested that the cell-wall process described represents an excretory mechanism of , and the nature of the products released by the young vibrios and their probable relation with cholera toxin is discussed.

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1967-10-01
2024-03-29
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References

  1. Aberchrombie N., Hickman C. J., Johnson M. L. 1957 A Dictionary of Biology Aylesbury: Hunt, Barnard and Co. Ltd;
    [Google Scholar]
  2. Bennett H. S. 1956; The concepts of membrane flow and membrane vesiculation as mechanisms for active transport and ion pumping. J. biophys. biochem. Cytol299
    [Google Scholar]
  3. Bernard P. N., Gallut J. 1943; Sur une mode de préparation de la toxine cholérique. C. r. Séanc. Soc. Biol 137:10
    [Google Scholar]
  4. Bhatia R. Y. P., Kaur J., Bhatia A. L., Shrivastava J. B. 1966; Choleragenic property of lipopolysaccharide fraction from Vibrio cholerae Inaba. Indian J. med. Res 54:323
    [Google Scholar]
  5. Borysko E., Sapranauskas P. 1954; A new technique for comparative phase contrast and electron microscope studies of cells grown in tissue culture with an evaluation of the technique by means of time lapse cinemicrographs. Bull. Johns Hopkins Hosp 95:68
    [Google Scholar]
  6. Burrows W. 1951; Endotoxins. A. Rev. Microbiol 5:181
    [Google Scholar]
  7. Burrows W. 1965; Cholera vibrio toxicities. Proc. Cholera Res. SympHonolulu120
    [Google Scholar]
  8. Chatterjee S. N., Das J. 1966; Secretory activity of Vibrio cholerae as evidenced by electron microscopy. In Electron Microscopy Uyeda R. 11OO–259 Tokyo: Maruzen Co. Ltd;
    [Google Scholar]
  9. Das J., Chatterjee S. N. 1966; Electron microscopic studies on some ultrastructural aspects of Vibrio cholerae . Indian J. med. Res 54:330
    [Google Scholar]
  10. De S. N., Ghose M. L., Sen A. 1960; Activities of bacteria-free preparations from Vibrio cholerae . J. Path. Bact 79:373
    [Google Scholar]
  11. De S. N., Ghose M. L., Chandra J. 1962; Further observations on cholera enterotoxin. Trans. R. Soc. trop. Med. Hyg 56:241
    [Google Scholar]
  12. Dutta N. K., Habbu M. K. 1955; Experimental cholera in infant rabbits. A method for chemotherapeutic investigation. Br. J. Pharmacol. Chemother 10:153
    [Google Scholar]
  13. Dutta N. K., Oza N. B. 1963; The effect of gastro-intestinal enzymes on cholera toxin. Bull. Wld Hlth Org 28:307
    [Google Scholar]
  14. Epstein M. A. 1962; Functional aspects of the structure of some animal viruses. Br. med. Bull 18:183
    [Google Scholar]
  15. Farquhar M. G. 1961a; Fine structure and function in capillaries of anterior pituitary gland. Angiology 12:270
    [Google Scholar]
  16. Farquhar M. G. 1961b Trans. N.Y. Acad. Sci 23:346 Lever. 1962
    [Google Scholar]
  17. Finkelstein R. A., Norris H. T., Dutta N. K. 1964; Pathogenesis of experimental cholera in infant rabbits. J. infect. Dis 114:203
    [Google Scholar]
  18. Finkelstein R. A. 1965; Observations on the nature and mode of action of the choleragenic produces) of cholera vibrios. Proc. Cholera Res. SympHonolulu264
    [Google Scholar]
  19. Finkelstein R. A., Atthasampunna P., Chulasamaya M., Charunmethee P. 1966; Pathogenesis of experimental cholera: biologic activities of purified procholeragen A. J. Immun 96:440
    [Google Scholar]
  20. Gallut J., Grabar P. 1945; Recherches immunochimiques sur de vibrion cholérique. III. Mise en évidence de deux constituants toxiques de nature différente dans la toxine cholérique. Annls Inst. Pasteur, Paris 71:83
    [Google Scholar]
  21. Gallut J. 1954; Contribution a l’étude de la toxine cholérique: Variation du pouvoir toxique de Vibrio cholerae (Ogawa) au cours de la maladie. Annls Inst. Pasteur, Paris 86:561
    [Google Scholar]
  22. Gallut J. 1965; Antigenic structure of vibrios. Proc. Cholera Res. SympHonolulu235
    [Google Scholar]
  23. Glauert A. M., Kerrtdge D., Horne R. W. 1963; The fine structure and the mode of attachment of the sheathed flagellum of Vibrio metchnikovii . J. Cell Biol 18:327
    [Google Scholar]
  24. Huxley H. E., Zubay G. 1960; Electron microscope observations on the structure of particles from Escherichia coli . J. molec. Biol 2:10
    [Google Scholar]
  25. Kellenberger E., Ryter A., Séchaud J. 1958; Electron microscope study of DNA plasms. J. biophys. biochem. Cytol 4:671
    [Google Scholar]
  26. Knox K. N., Vesk M., Work E. 1966; Relation between excreted lypolysaccharide complex and surface structures of a lysine-limited culture of Escherichia coli . J. Bact 92:1206
    [Google Scholar]
  27. Kurosumi K. 1961; Electron microscopic analysis of the secretion mechanism. Int. Rev. Cytol II:1
    [Google Scholar]
  28. Kurosumi K. 1962; Some morphological aspects on the secretory activities of various glandular cells. In Electron Microscopy Breese S. S. IIYY–1 New York and London: Academic Press;
    [Google Scholar]
  29. Lawn A. M. 1960; The use of potassium permanganate as an electron dense stain for sections of tissue embedded in epoxy resin. J. biophys. biochem. Cytol 7:197
    [Google Scholar]
  30. Lever J. D., Peterson R. 1960 Trans. N.Y. Acad. Sci 22:504 Lever. 1962
    [Google Scholar]
  31. Lever J. D. 1961 In Electron Microscopy in Anatomy Boyd J. D., Johnson F. R., Lever J. D. 207 London: Arnold; Lever. 1962
    [Google Scholar]
  32. Lever J. D. 1962; Fine structural organisation in endocrine tissues. Br. med. Bull 18:3229
    [Google Scholar]
  33. Lewis W. H. 1931; Pinocytosis. Bull. Johns Hopkins Hosp 49:17
    [Google Scholar]
  34. Luft J. H. 1961; Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol 9:409
    [Google Scholar]
  35. Millonig G. 1961; A modified procedure for lead staining of thin sections. J. biophys. biochem. Cytol 11:736
    [Google Scholar]
  36. Palade G. E. 1953; Fine structure of blood capillaries. J. appl. Phys 24:1424
    [Google Scholar]
  37. Palade G. E. 1959; Functional modulations in cell structure. In Sub-cellular particles Hayashi T. 264 New York: Ronald Press;
    [Google Scholar]
  38. Palade G. E. 1960; Transport in quanta across the endothelium of blood capillaries. Anat. Rec 136:254
    [Google Scholar]
  39. Palade G. E. 1961 In Electron Microscopy in Anatomy Boyd J. D., Johnson F. R., Lever J. D. 176 London: Arnold; Lever. 1962
    [Google Scholar]
  40. Pollitzer R. 1959; Cholera. Monograph Ser W.H.O;43
    [Google Scholar]
  41. Proceedings of the Cholera Research SymposiumHonolulu 1965 Washington, U.S.A.: Dept Hlth, Education & Welfare;
    [Google Scholar]
  42. Reynolds E. S. 1963; The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J. Cell Biol 17:208
    [Google Scholar]
  43. Watson M. L. 1957; Reduction of heating artifacts in thin sections examined in electron microscope. J. biophys. biochem. Cytol 3:1017
    [Google Scholar]
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