1887

Abstract

SUMMARY: A study (Fincham, 1950 of two mutant strains of , which probably owed their origin to independent occurrences of mutation at the same locus, showed them to be deficient in their ability to synthesize from ammonia the -amino groups of a wide range of amino-acids. They grew well when any one of a number of different -amino-acids was added to the medium, but would only grow slowly, and after a prolonged lag-phase, in the absence of amino-acid. The corresponding -amino-acids and -keto-acids were inactive in promoting growth. The mutants were subnormal in their capacity to assimilate ammonium nitrogen. In the present study the -glutamic acid dehydrogenase activity of dialysed extracts of strains carrying the amination-deficiency mutation, and of a number of other wild-type and mutant strains, was investigated. All strains, with the exception of the amination-deficient strains, yielded an -glutamic acid dehydrogenase specific for coenzyme II. Extracts of four amination-deficient strains had no detectable glutamic dehydrogenase activity, and did not inhibit the enzyme appreciably when added to wild-type extracts. Glucose-6-phosphate dehydrogenase was present in one extract tested for its presence.

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/content/journal/micro/10.1099/00221287-5-4-793
1951-12-01
2024-05-10
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References

  1. Adler E., Günther G., Everett J. E. 1938; Über den enzymatischen Abbau und Aufbau der Glutaminsäure. IV. In Hefe. Hoppe-Seyl. Z 255:27
    [Google Scholar]
  2. Adler E., Hellström V., Günther G., Euler V. H. 1938; Über den enzymatischen Abbau und Aufbau der Glutaminsäure. III. In Bacterium coli. Hoppe-Seyl. Z 255:14
    [Google Scholar]
  3. Beadle G. W., Tatum E. L. 1945; Neurospora. II. Methods of producing and detecting mutations concerned with nutritional requirements. Amer. J. Bot 32:678
    [Google Scholar]
  4. Bonner D. M. 1946; Biochemical mutations in Neurospora. Cold Spr. Harb. Sym. quant. Biol 11:14
    [Google Scholar]
  5. Cammarata P. S., Cohen P. P. 1950; The scope of the transamination reaction in animal tissues. J. biol. Chem 187:439
    [Google Scholar]
  6. Conway E. J., Byrne A. 1933; An absorption apparatus for the microdetermination of certain volatile substances. I. The micro-determination of ammonia. Biochem. J 27:419
    [Google Scholar]
  7. Feldman L. I., Gunsalus I. C. 1950; The occurrance of a wide variety of transaminases in bacteria. J. biol. Chem 187:821
    [Google Scholar]
  8. Fincham J. R. S. 1950; Mutant strains of Neurospora deficient in animating ability. J. biol. Chem 182:61
    [Google Scholar]
  9. Gordon M., Mitchell H. K. 1950; Tryptophane desmolase in Neurospora. Genetics 35:110
    [Google Scholar]
  10. Le Page G. A. 1949 Umbreit W. W., Burris R. H., Stauffer J. F. Manometric Techniques and Tissue Metabolism Minneapolis: Burgess Publishing Co;
    [Google Scholar]
  11. Le Page G. A., Mueller G. C. 1949; Preparation of T.P.N. J. biol. Chem 180:975
    [Google Scholar]
  12. Lewis R. W. 1948; Mutants of Neurospora requiring succinic acid or a biochemically related acid for growth. Amer. J. Bot 35:292
    [Google Scholar]
  13. Mitchell H. K., Lein J. 1948; A Neurospora mutant deficient in the enzymatic synthesis of tryptophan. J. biol. Chem 175:481
    [Google Scholar]
  14. Sealock R. R. 1941; The determination of amino-acids of the dextro or unnatural configuration. Science 94:73
    [Google Scholar]
  15. Srb A. M., Fincham J. R. S., Bonner D. M. 1950; Evidence from gene mutations in Neurospora for close metabolic relationships among ornithine, proline and α-amino δ-hydroxy valeric acid. Amer. J. Bot 37:533
    [Google Scholar]
  16. Wagner R. P. 1949; The in vitro synthesis of pantothenic acid by pantothenicless and wild type Neurospora. Proc. nat. Acad. Sci. Wash 35:185
    [Google Scholar]
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