1887

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Volume 5, Issue 5

The Assay of the Antibiotic Nisin by Means of a Reductase (Resazurin) test Free

Abstract

Summary: The familiar resazurin test used in milk testing has been adapted to the estimation of nisin, using a fast-growing strain of as test organism. The reproducibility and accuracy of this test compares favourably with the many other assay methods for nisin which use as test organism. Results may be obtained in less than an hour as compared with 15–48 hr. in the other tests and are a measure of bactericidal power only. Aseptic technique during the actual assay procedure is unnecessary. Methods were devised to ensure a suitable standard inoculum, which is imperative for this test. As little as 0·5 unit nisin ml, may be estimated.

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© Society for General Microbiology, 1951
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/content/journal/micro/10.1099/00221287-5-5-830
1951-12-01
2024-04-23
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References

  1. Bavin E.M., Falconer R., Friedmann R., Robson J.M. 1951; Nisin in experimental tuberculosis. (In preparation.).
    [Google Scholar]
  2. Davis J.G. 1939; Grading milk by Resazurin test. Food Manuf. 14:196
     [Google Scholar]
  3. Friedmann R., Beach S.A. 1950; New methods of assay for the antibiotic nisin. gen. Microbiol. 5:v
     [Google Scholar]
  4. Hirsch A. 1950; The assay of nisin. J. gen. Microbiol. 4:70
     [Google Scholar]
  5. Mattick A.T.R., Hirsch A. 1947; Further observations on an inhibitory substance (nisin) from lactic streptococci. Lancet ii:5
     [Google Scholar]
  6. Ministry of agriculture fisheries 1943; National milk testing and advisory scheme: The routine Resazurin test. Technique nos. C158/T.P.Y. and Cl50/T.P.Y.
  7. Pesch K.L., Simmert. 1929; Untersuchungen über den Frischheitszustand der Milch. Bestimmung der Wasserstoffionenkonzentration und der Reduktionszeit. Milchw. Forsch. 8:551
     [Google Scholar]
  8. Reid R.D., Brewer J.H. 1946; The reductase method for the determination of penicillin concentration in body fluids. J. Bact 52:251
     [Google Scholar]
  9. Sanchez G., Lamensans A. 1947; Méthode de dosage rapide de la pénicilline et de la streptomycine. C.R. Acad. Sci., Paris 224:1189
     [Google Scholar]
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The Assay of the Antibiotic Nisin by Means of a Reductase (Resazurin) test
Microbiology 5, 830 (1951); https://doi.org/10.1099/00221287-5-5-830
/content/journal/micro/10.1099/00221287-5-5-830
/content/journal/micro/10.1099/00221287-5-5-830
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