RT Journal Article SR Electronic(1) A1 Sijpesteijn, A. KaarsYR 1951 T1 On Ruminococcus flavefaciens, a Cellulose-decomposing:Bacterium from the Rumen of Sheep and Cattle JF Microbiology, VO 5 IS 5 SP 869 OP 879 DO https://doi.org/10.1099/00221287-5-5-869 PB Microbiology Society, SN 1465-2080, AB Summary: Two strains of Ruminococcus flavefaciens, an important cellulose-decomposing bacterium, were isolated, one from the rumen of a sheep, the other from the rumen of a cow. Pure cultures were obtained by using the dilution method in agar media containing a strip of filter-paper. These strictly anaerobic, Gram-positive streptococci attack cellulose and cellobiose, but not starch, maltose, lactose or xylose. Only one strain could use glucose. Colonies on cellulose media were characterized by the formation of a yellow pigment; in cellobiose media the colonies were white. Growth on cellulose was favoured by addition of Clostridium sporogencs or a certain amount of sterilized medium in which Cl. sporogenes had previously grown. Estimations of the end-products of fermentation of cellulose and cellobiose showed that at least 25% of the carbon could be recovered as succinic acid, c. 23% as acetic acid and c. 10% as formic acid; ethanol was absent and gas formation very limited. A description of the genus and the species is given., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-5-5-869