Butyramide-utilizing Mutants of Pseudomonas aeruginosa 8602 which Produce an Amidase with Altered Substrate Specificity BROWN, JANE E. and BROWN, P. R. and CLARKE, PATRICIA H.,, 57, 273-285 (1969), doi = https://doi.org/10.1099/00221287-57-2-273, publicationName = Microbiology Society, issn = 1350-0872, abstract= SUMMARY Mutants of Pseudomonas aeruginosa 8602 were isolated which, unlike the wild type, were able to grow with butyramide as a carbon source. Six mutants derived from the constitutive strain c 11 were shown to produce an enzyme (B amidase) with altered electrophoretic mobility and altered substrate specificity. The apparent K m for butyramide of the B amidase was about a tenth of that of the A amidase and the V max was about ten-fold greater. A further mutation produced mutants able to grow on valeramide., language=, type=