1887

Abstract

Summary: A series of biochemical methods using heavy suspensions of organisms and chemically-defined solutions are described; they include fermentations, reduction of nitrate and of methylene blue, production of indole, hydrogen sulphide and acetoin, and hydrolysis of gelatin, starch and urea. The tests use the preformed enzymes of the bacterial cells and the results are not complicated by side effects or the multiple reactions that occur in cultures growing in a nutrient medium containing the test substrate.

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/content/journal/micro/10.1099/00221287-6-1-2-187
1952-02-01
2024-05-08
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References

  1. Arnold W.M. Jun. Weaver R.H. 1948; Quick microtechniques for the identification of cultures. I. Indole production. J.Lab. din. Med. 33:1334
    [Google Scholar]
  2. Bachmann B., Weaver R.H. 1951; Rapid microtechnics for identification of cultures. V. Reduction of nitrates to nitrites. Amer. J. din. Path. 21:195
    [Google Scholar]
  3. Barritt M.M. 1936; The intensification of the Voges-Proskauer reaction by the addition of α-naphthol. J. Path. Boot. 42:441
    [Google Scholar]
  4. Batty-Smith C.G. 1941; The detection of acetyl-methyl-carbinol in bacterial cultures. A comparative study of the methods of O’Meara and of Barritt. J. Hyg., Camb. 41:521
    [Google Scholar]
  5. Brough F.K. 1950; A rapid microtechnique for the determination of nitrate reduction by micro-organisms. J. Bact. 60:365
    [Google Scholar]
  6. Cook G.T. 1948; Urease and other biochemical reactions of the Proteus group. J. Path. Bact. 60:171
    [Google Scholar]
  7. Davis J.G. 1939; A rapid, simple and reproducible method for the determination of bacterial sugar fermentations. Zbl. Bakt. 2 Abt. 101:97
    [Google Scholar]
  8. Davis J.G., Rogers H.J. 1939; The effect of sterilisation upon sugars. Zbl. Bakt. 2 Abt. 101:102
    [Google Scholar]
  9. Donald R. 1913; An apparatus for liquid measurement by drops and applications in counting bacteria and other cells and in serology, etc. Proc. Boy. Soc.B 86:198
    [Google Scholar]
  10. Elek S.D. 1948; Rapid identification of Proteus. J. Path. Bact. 60:183
    [Google Scholar]
  11. Evans W.C., Handley W.R.C., Happold F.C. 1941; The tryptophanase-indole reaction. 4. Biochem. J. 35:207
    [Google Scholar]
  12. Fabrizio A., Weaver R.H. 1951; Rapid microtechnics for identification of cultures. IV. Acetylmethylcarbinol production. Amer. J. clin. Path. 21:192
    [Google Scholar]
  13. Frazier W.C. 1926; A method for the detection of changes in gelatin due to bacteria. J. infect. Dis. 39:302
    [Google Scholar]
  14. Galton M.M., Hardy A.V., Mitchell R.B. 1950; The public health laboratory diagnosis of enteric infections. Amer. J. trop. Med. 30:77
    [Google Scholar]
  15. Hannan J., Weaver R.H. 1948; Quick microtechniques for the identification of cultures. II. Fermentations. J. Lab. clin. Med. 33:1338
    [Google Scholar]
  16. Harden A. 1906; On Voges and Proskauer’s reaction for certain bacteria. Proc. Boy. Soc.B 77:424
    [Google Scholar]
  17. Hargrove R.E., Weaver R.H. 1951; Rapid microtechnics for identification of cultures. VI. Citrate utilization. Amer. J. clin. Path. 21:286
    [Google Scholar]
  18. Kluyver A.J., Donker H.J.L., Visser’T Hooft F. 1925; Über die Bildung von Acetylmethylcarbinol und 2,3-Butylenglycol im Stoffweehsel der Hefe. Biochem. Z. 161:361
    [Google Scholar]
  19. Langlykke A.F., Peterson W.H. 1937; The determination of acetylmethylcarbinol. Industr. Engng Chem. Anal, ed. 9:163
    [Google Scholar]
  20. Lemoigne M. 1920; Réaction spècifique du 2.3-butylèneglycol et de 1’acétyl-méthylcarbinol, produits de la fermentation butylène glycolique. C.B. Acad. Sci., Paris 170:131
    [Google Scholar]
  21. Miles A.A., Misra S.S. 1938; The estimation of the bactericidal power of the blood. J. Hyg., Camb. 38:732
    [Google Scholar]
  22. Morse M.L., Weaver R.H. 1950; Rapid microtechnics for identification of cultures. III. Hydrogen sulfide production. Amer. J. clin. Path. 20:481
    [Google Scholar]
  23. Oakley C.L., Warrack G.H., Warren M.E. 1948; The kappa and lambda antigens of Clostridium welchii. . J. Path. Bact. 60:495
    [Google Scholar]
  24. Silverman M., Werkman C.H. 1941; The formation of acetylmethylcarbinol from pyruvic acid by a bacterial enzyme preparation. J. biol. Chem. 138:35
    [Google Scholar]
  25. Wallace G.I., Neave S.L. 1927; The nitrite test as applied to bacterial cultures. J. Bact. 14:377
    [Google Scholar]
  26. Zobell C.E. 1932; Factors influencing the reduction of nitrates and nitrites by bacteria in semisolid media. J. Bact. 24:273
    [Google Scholar]
  27. Zobell C.E., Feltham C.B. 1934; A comparison of lead, bismuth, and iron as detectors of hydrogen sulphide produced by bacteria. J. Bact. 28:169
    [Google Scholar]
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