1887

Abstract

SUMMARY: Hydrogen sulphide production by growing cultures and non-multiplying suspensions was compared and the factors influencing the sensitivity of the tests were investigated. Cysteine hydrochloride (0·01 %) was added to Lemco broth to provide a medium with a standard source of sulphur. HS was detected with lead acetate papers more readily than by lead acetate agar.

Suspensions were tested with cysteine, sodium thiosulphate and sodium sulphite; the organisms investigated were mainly Bacteriaceae and seldom failed to produce HS from cysteine; sodium thiosulphate was less readily attacked; sodium sulphite was unsuitable for this technique. Media commonly used for testing the capacity of bacteria to produce HS are reviewed and the value of this test in bacterial classification discussed.

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1953-06-01
2024-04-23
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References

  1. Almy L. H. 1925; A method forthe estimation of hydrogen sulfide in proteinaceous food products. J. Amer. chem. Soc 47:1381
    [Google Scholar]
  2. Bailey S. F., Lacey G. R. 1927; A modification of the Kligler lead acetate medium. J. Bact 13:183
    [Google Scholar]
  3. Beckwith T. D., Moser J. R. 1932; The reduction of sulphur containing compounds in wood pulp and paper manufacture. J. Bact 24:43
    [Google Scholar]
  4. Bürger M. 1914; Über Schwefelwasserstoffbildung aus Zystin durch Bakterien. Arch. Hyg., Berl 82:201
    [Google Scholar]
  5. Burnet É., Weissenbach R. J. 1915; Yaleur des renseignements foumis par la culture en gélose a l’acétate de plomb, pour la différenciation des bacilles typhique, paratyphique A et paratyphique B. C.R. Soc. Biol., Paris 78:565
    [Google Scholar]
  6. Clarke P. H., Cowan S. T. 1952; Biochemical methods for bacteriology. J. gen. Microbiol 6:187
    [Google Scholar]
  7. Cook G. T. 1952; Comparison of two modifications of bismuth-sulphite agar for the isolation and growth of Salmonella typhi and Salmonella typhimurium. J. Path. Bact 64:559
    [Google Scholar]
  8. Desnuelle P., Fromageot C. 1939; La decomposition anaerobie de la cysteine par Bacterium coli. Enzymologia 6:80
    [Google Scholar]
  9. Desnuelle P., Wookey E., Fromageot C. 1940; Sur la dégradation anaérobie de la cystéine et de la cystine par Propionibaderium pentosaceum. Enzymologia 8:225
    [Google Scholar]
  10. Friewer F., Shaughnessy H. J. 1944; Lead semisolid agar, a medium for use in the identification of the enteric group of bacteria. Amer. J. clin. Path. tech. Suppl 14:1
    [Google Scholar]
  11. Fromageot C. 1951; Desulfhydrases. The Enzymes 1 pt. 2 p. 1237 New York: Academic Press Inc;
    [Google Scholar]
  12. Hajna A. A. 1945; Triple-sugar iron agar medium for the identification of the intestinal group of bacteria. J. Bact 49:516
    [Google Scholar]
  13. Heap H., Cadness B. H. E. 1924-5; The influence of carbohydrates on hydrogen sulphide production by Bacillus aertrycke (Mutton). J. Hyg., Camb 23:77
    [Google Scholar]
  14. Huddleson I. F. 1929; The differentiation of the species of the genus Brucella. Tech. Bull. Mich, agric. Exp. Sta100
    [Google Scholar]
  15. Hunter C. A., Crecelius H. G. 1938; Hydrogen sulphide studies. I. Detection of hydrogen sulphide in cultures. J. Bact 35:185
    [Google Scholar]
  16. Hunter C. A., Feldman M., Crecelius G. 1937; Detection of hydrogen sulphide in cultures. J. Bact 33:31
    [Google Scholar]
  17. Hunter C. A., Weiss J. E. 1938; Comparative studies of methods for the detection of hydrogen sulphide in the coli-aerogenes group. J. Bact 35:20
    [Google Scholar]
  18. Jordan E. O., Victorson R. 1917; Differentiation of the paratyphoid-enteritidis group. II. Lead acetate agar. J. infect. Dis 21:554
    [Google Scholar]
  19. Kauffmann F. 1951 Enterobacteriaceae Copenhagen: Ejnar Munksgaard;
    [Google Scholar]
  20. Kligler I. J. 1917; A simple medium for the differentiation of members of the typhoid-paratyphoid group. Amer. J. publ. Hlth 7:1042
    [Google Scholar]
  21. Kligler I. J. 1918; Modifications of culture media used in the isolation and differentiation of typhoid, dysentery, and allied bacilli. J. exp. Med 28:319
    [Google Scholar]
  22. Knox R. 1949; A screening plate for the rapid identification of faecal organisms. J. Path. Bact 61:343
    [Google Scholar]
  23. Levine M., Vaughn R., Epstein S. S., Anderson D. Q. 1931–2; Some differential reactions in the colon-aerogenes group of bacteria. Proc. Soc. exp. Biol., N.Y 29:1022
    [Google Scholar]
  24. Morrison L. E., Tanner F. W. 1922; Studies on thermophilic bacteria. I. Aerobic thermophilic bacteria from water. J. Bact 7:343
    [Google Scholar]
  25. Myers J. T. 1920; The production of hydrogen sulphide by bacteria. J. Bact 5:231
    [Google Scholar]
  26. Orlowski M. A. 1895; Hydrogène sulfuré comme produit de eertaines baetéries. J. med. milit. Russe, cited (1897) in Jber. Fortschr. path. Mikroorg 11:528
    [Google Scholar]
  27. Pacheco G., Mello J. L. 1932; Sur un procédé de détermination de l’hydrogène sulfuré dans les cultures bactériennes. C.R. Soc. Biol., Paris 110:131-2
    [Google Scholar]
  28. Patrick R., Werkman C. H. 1933; Bacteria fermenting xylan. Iowa St. Coll. J. Sci 7:407
    [Google Scholar]
  29. Sasaki T., Otsuka I. 1912; Experimentelle Untersuchungen über die Sehwefel-wasserstoffentwicklung der Bakterien aus Cystin und sonstigen Schwefelver-bindungen. Biochem. Z 39:208
    [Google Scholar]
  30. Seeliger H. 1950; Über die Identitat und Stellung des B paradysenteriae palatinense (Roelke). Z. Hyg. InfektKr 130:490
    [Google Scholar]
  31. Society Of American Bacteriologists 1947 Manual of methods for pure culture study of bacteria Leaflet v p. 13 Geneva, N.Y: Biotechnic Publications;
    [Google Scholar]
  32. Spray R. S. 1936; Semisolid media for cultivation and identification of the sporulating anaerobes. J. Bact 32:135
    [Google Scholar]
  33. Sulkin S. E., Willett J. C. 1939-40; A triple sugar-ferrous sulfate medium for use in identification of enteric organisms. J. Lab. clin. Med 25:649
    [Google Scholar]
  34. Tabet F. 1938; A modification of Wilson and Blair’s bismuth medium suitable for both typhoid and paratyphoid bacilli. J. Path. Bact 46:181
    [Google Scholar]
  35. Tanner F. W. 1917; Studies on the bacterial metabolism of sulfur. I. Formation of hydrogen sulfide from certain sulfur compounds under aerobic conditions. J. Bact 2:585
    [Google Scholar]
  36. Tarr H. L. A. 1933a; The anaerobic decomposition of L-cystine by washed cells of Proteus vulgaris. Biochem. J 27:759
    [Google Scholar]
  37. Tarr H. L. A. 1933b; The enzymic formation of hydrogen sulphide by certain heterotrophic bacteria. Biochem. J 27:1869
    [Google Scholar]
  38. Tarr H. L. A. 1934; The enzymic formation of hydrogen sulphide by certain heterotrophic bacteria. II. Biochem. J 28:192
    [Google Scholar]
  39. Thompson L. S. 1920-21; The group of hydrogen sulphide producing bacteria. J. med. Res 42:383
    [Google Scholar]
  40. Tilley F. W. 1923a; Variations in hydrogen sulphide production by bacteria. J. Bact 8:115
    [Google Scholar]
  41. Tilley F. W. 1923b; The relation between chemical composition of peptones and hydrogen sulphide production by bacteria. J. Bact 8:287
    [Google Scholar]
  42. Tittsler R. P., Sandholzer L. A. 1937; Advantages of peptone iron agar for the routine detection of hydrogen sulphide production. Amer. J. publ. Hlth 27:1240
    [Google Scholar]
  43. Utermohlen W. P. Jun Georgi C. E. 1940; A comparison of cobalt and nickel salts with other agents for the detection of hydrogen sulfide in bacterial cultures. J. Bact 40:449
    [Google Scholar]
  44. Vaughn R., Levine M. 1936; Hydrogen sulfide production as a differential test in the colon group. J. Bact 31:24
    [Google Scholar]
  45. Wilmet M. 1927; Sur la sensibilité de quelques réactifs de l’hydrogène sulphuré gazeux. C.R. Acad. Sci., Paris 184:287
    [Google Scholar]
  46. Wilson W. J. 1922-3; Reduction of sulphites by certain bacteria in media containing a fermentable carbohydrate and metallic salts. J. Hyg., Camb 21:392
    [Google Scholar]
  47. Wilson W. J. 1938; Isolation of Bact. typhosum by means of bismuth sulphite medium in water and milk borne epidemics. J. Hyg., Camb 38:507
    [Google Scholar]
  48. Wilson W. J., Blair E. M. McV. 1927; Use of a glucose, bismuth, sulphite, iron medium for the isolation of B. typhosus and B. proteus. J. Hyg., Camb 26:374
    [Google Scholar]
  49. Zobell C. E., Feltham C. B. 1934; A comparison of lead, bismuth and iron as detectors of hydrogen sulphide produced by bacteria. J. Bact 28:169
    [Google Scholar]
  50. Zobell C. E., Meyer K. F. 1932; Metabolism studies on the Brucella group. V. The production of hydrogen sulphide. J. infect. Dis 51:91
    [Google Scholar]
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