@article{mbs:/content/journal/micro/10.1099/00221287-83-2-311, author = "Matin, A. and Konings, W. N. and Kuenen, J. G. and Emmens, M.", title = "Active Transport of Amino Acids by Membrane Vesicles of Thiobacillus neapolitanus", journal= "Microbiology", year = "1974", volume = "83", number = "2", pages = "311-318", doi = "https://doi.org/10.1099/00221287-83-2-311", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-83-2-311", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "Summary: Membrane vesicles of Thiobacillus neapolitanus take up amino acids at 25 °C in the presence of the nonphysiological electron donor, ascorbate- N,N,N′,N′-tetramethyl- p -phenylenediamine. The amino acids accumulate inside the membrane vesicles against a concentration gradient. Inhibitors of the electron transport chain inhibit the accumulation; therefore active transport of amino acids in T. neapolitanus is coupled to the electron transport chain. The Km values for the transport of glycine and l-serine in this organism are 2·5 and 5 μ m respectively.", }