Active Transport of Amino Acids by Membrane Vesicles of Thiobacillus neapolitanus Matin, A. and Konings, W. N. and Kuenen, J. G. and Emmens, M.,, 83, 311-318 (1974), doi = https://doi.org/10.1099/00221287-83-2-311, publicationName = Microbiology Society, issn = 1350-0872, abstract= Summary: Membrane vesicles of Thiobacillus neapolitanus take up amino acids at 25 °C in the presence of the nonphysiological electron donor, ascorbate- N,N,N′,N′-tetramethyl- p -phenylenediamine. The amino acids accumulate inside the membrane vesicles against a concentration gradient. Inhibitors of the electron transport chain inhibit the accumulation; therefore active transport of amino acids in T. neapolitanus is coupled to the electron transport chain. The Km values for the transport of glycine and l-serine in this organism are 2·5 and 5 μ m respectively., language=, type=