RT Journal Article SR Electronic(1) A1 KINGHORN, J. R. A1 PATEMAN, J. A.YR 1975 T1 The Structural Gene for NADP l-Glutamate Dehydrogenase in Aspergillus Nidulans JF Microbiology, VO 86 IS 2 SP 294 OP 300 DO https://doi.org/10.1099/00221287-86-2-294 PB Microbiology Society, SN 1465-2080, AB SUMMARY: A total of 41 mutants lacking NADP l-glutamate dehydrogenase (NADP-GDH) activity have been studied. All the mutations were located at the gdhA locus within 0·1% recombination of gdhA1. Two mutants, gdhA1 and gdhA2, out of five examined, produced cross-reacting material which neutralized NADP-GDH antiserum. The mutant gdhA9 has altered Km values for all five substrates: ammonium, α-ketoglutarate, l-glutamate, NADPH and NADP. The mutant gdhA20 had temperature-sensitive growth, abnormal ammonium-regulation characteristics and thermolabile NADP-GDH activity. These results show that gdhA is the structural gene for NADP-GDH., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-86-2-294