1887

Abstract

SUMMARY: During aerobic growth of ML308 on acetate as sole carbon source, the apparent synthesis of isocitrate dehydrogenase was repressed relative to cultures on other carbon sources, such as glucose, which do not employ the glyoxylate bypass as an anaplerotic sequence. When cells were removed from an acetate medium, or when compounds were added which made the operation of the glyoxylate bypass unnecessary, the activity of isocitrate dehydrogenase rapidly increased 3- to 4-fold but fell again on restoration to an acetate medium. Changes in activity were rapid and, furthermore, could be demonstrated in the absence of protein synthesis. It is thus improbable that the mechanism involved degradation or synthesis of the enzyme protein. Oxaloacetate and glyoxylate showed concerted inhibition of isocitrate dehydrogenase which could be relieved by dialysis. Because extracts of low enzyme activity, derived from acetate-metabolizing cells, could not be stimulated by dialysis or by addition of a wide range of metabolites, it is unlikely that low molecular weight, freely dissociable effectors were responsible for stimulation or inhibition of activity.

Control of isocitrate dehydrogenase permitted the efficient utilization of acetate as sole source of carbon and energy but preserved the capacity of the cell to respond rapidly to an improvement in nutritional conditions. A limited survey showed that the mechanism is common but not universal among strains of and occurs in at least one strain each of and

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-87-1-37
1975-03-01
2024-04-27
Loading full text...

Full text loading...

/deliver/fulltext/micro/87/1/mic-87-1-37.html?itemId=/content/journal/micro/10.1099/00221287-87-1-37&mimeType=html&fmt=ahah

References

  1. Ashworth J. M., Kornberg H. L. 1963; Fine control of the glyoxylate cycle by allosteric inhibition of isocitrate lyase. Biochimica et biophysica acta 73:519–522
    [Google Scholar]
  2. BéChet J., Wiame J. M. 1965; Indication of a specific regulatory binding protein for ornithine trans- carbamylase in Saccharomyces cerevisiae. Biochemical and Biophysical Research Communications 21:226–234
    [Google Scholar]
  3. Bennett P. M., Holms W. H. 1970; Regulation of isocitrate dehydrogenase activity in Escherichia coli by the operation of the glyoxylate cycle. Journal of General Microbiology 61:ix–x
    [Google Scholar]
  4. Bessman S. P. 1957; Preparation and assay of oxalacetic acid. In Methods in Enzymology 3 pp. 418–421 Colowick S. P., Kaplan N. O. Edited by New York: Academic Press;
    [Google Scholar]
  5. Britten R. J. 1954; Extracellular metabolic products of Escherichia coli during rapid growth. Science; New York: 119578
    [Google Scholar]
  6. Brown D. H., Cori C. F. 1961; Animal and plant polysaccharide phosphorylases. In The Enzymes 5 pp. 207–228 Boyer P. D., Lardy H., Myrback K. Edited by New York and London: Academic Press;
    [Google Scholar]
  7. Hadjipetrou L. P., Gerrits J. P., Teulings F. A. G., Stouthamer A. H. 1964; Relation between energy production and growth of Aerobacter aerogenes. Journal of General Microbiology 36:139–150
    [Google Scholar]
  8. Halpern Y. S., Umbarger H. E. 1960; Conversion of ammonia to amino groups in Escherichia coli. Journal of Bacteriology 80:285–288
    [Google Scholar]
  9. Holms W. H., Bennett P. M. 1971; Regulation of isocitrate dehydrogenase activity in Escherichia coli on adaptation to acetate. Journal of General Microbiology 65:57–68
    [Google Scholar]
  10. Kay W. W., Kornberg H. L. 1969; Genetic control of the uptake of C4-dicarboxylic acids by Escherichia coli. FEBS Letters 3:93
    [Google Scholar]
  11. Kogut M., Podoski E. P. 1953; Oxidative pathways in a fluorescent Pseudomonas. Biochemical Journal 55:800–811
    [Google Scholar]
  12. Kornberg H. L. 1966; The role and control of the glyoxylate cycle in Escherichia coli. Biochemical Journal 99:1–11
    [Google Scholar]
  13. Kornberg H. L., Collins J. F., Bigley D. 1960; The influence of growth substrates on metabolic pathways in Micrococcus denitrificans. Biochimica et biophysica acta 39:9–24
    [Google Scholar]
  14. Marr J. J., Weber M. M. 1969; Concerted inhibition of a NADP+-specific isocitrate dehydrogenase and the implications for metabolic regulation. Biochemical and Biophysical Research Communications 35:12–19
    [Google Scholar]
  15. Messenguy F., Béchet J., Wiame J. M. 1967; étude de Taction déune proteine regulatrice de léactivite de l’ornithine-carbamoyltransferase de Saccharomyces cerevisiae. Archives internationales de physiologie et de biochimie 75:889–891
    [Google Scholar]
  16. Messenguy F., Wiame J. M. 1969; The control of ornithine-transcarbamylase activity by arginase in Saccharomyces cerevisiae. FEBS Letters 3:47–49
    [Google Scholar]
  17. Ozaki H., Shiio I. 1968; Regulation of the TCA and glyoxylate cycles in Brevibacterium flavum. Journal of Biochemistry 64:355–363
    [Google Scholar]
  18. Reeves H. C., Brehmeyer B. A., Ajl S. J. 1968; Multiple forms of bacterial NADP+-specific isocitrate dehydrogenase. Science; New York: 162359–360
    [Google Scholar]
  19. Reeves H. C., Daumy G. O., Lin C. C., Houston M. 1972; NADP+-specific isocitrate dehydrogenase of Escherichia coli. I. Purification and characterization. Biochimica et biophysica acta 258:27–39
    [Google Scholar]
  20. Sekiguchi M., Iida S. 1967; Mutants of Escherichia coli permeable to actinomycin. Proceedings of the National Academy of Sciences of the United States of America 58:2315–2320
    [Google Scholar]
  21. Shapiro B. M., Stadtman E. R. 1970; The regulation of glutamine synthesis in microorganisms. Annual Review of Microbiology 24:501–524
    [Google Scholar]
  22. Shiio I., Ozaki H. 1968; Concerted inhibition of isocitrate dehydrogenase by glyoxylate plus oxalacetate. Journal of Biochemistry 64:45–53
    [Google Scholar]
  23. Swim H. E., Utter M. F. 1957; Isotopic experimentation with intermediates of the tricarboxylic acid cycle. In Methods in Enzymology 4 pp. 584–609 Colowick S. P., Kaplan N. O. Edited by New York: Academic Press;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-87-1-37
Loading
/content/journal/micro/10.1099/00221287-87-1-37
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error