1887

Abstract

Summary: Transductional analysis was applied to the mutant (). This mutant can utilize -histidinol as sole source of carbon and nitrogen and has a 60-fold increased histidinol dehydrogenase (HDH) content ( Dhawale, Creaser & Loper, 1972 ). Transductional analysis was carried out using 18 histidine-requiring mutants to see where the locus maps in relation to the structural genes of histidine biosynthesis. The marker cotransduced with group IV genes at 97 to 100 % and not at all with group I, which is known to be the structural gene for HDH.

The data obtained in the studies of (histidinol) and (NAD), and the effect of pH and temperature on the HDH activity from and are in full agreement with the genetic data that the mutation is not in the structural gene for HDH. It is suggested that may be a mutation in a regulatory gene affecting HDH synthesis in and may map close to whose function in histidine biosynthesis is not known.

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/content/journal/micro/10.1099/00221287-91-2-241
1975-12-01
2024-05-02
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References

  1. Ames B. N. 1957; The biosynthesis of histidine: d-erythro-imidazole-glycerol-phosphate dehydratase. Journal of Biological Chemistry 228:131–143
    [Google Scholar]
  2. Ames B. N., Hartman P. E., Jacob F. 1963; Chromosomal alterations affecting the regulation of histidine biosynthetic enzymes in Salmonella. Journal of Molecular Biology 7:23–42
    [Google Scholar]
  3. Creaser E. H., Bennett D. J., Drysdale R. B. 1967; The purification and properties of histidinol dehydrogenase from Neurospora crassa. Biochemical Journal 103:36–41
    [Google Scholar]
  4. Demerec M., Adelberg E. A., Clark A. J., Hartman P. E. 1966; A proposal for a uniform nomenclature in bacterial genetics. Genetics 5 4:61–76
    [Google Scholar]
  5. Dhawale M. R., Creaser E. H., Loper J. C. 1972; Evolutionary mechanism of adaptation of Arthro- bacter histidinolovorans and Pseudomonas aeruginosa to use L-histidinol as a sole source of nitrogen and carbon. Journal of General Microbiology 73:353–358
    [Google Scholar]
  6. Fargie B., Holloway B. W. 1965; Absence of clustering of functionally related genes in Pseudomonas aeruginosa. Genetical Research 6:284–299
    [Google Scholar]
  7. Holloway B. W., Krishnapillai V., Stanisich V. 1971; Pseudomonas genetics. Annual Review of Genetics 5:425–446
    [Google Scholar]
  8. Kay W. W., Gronlund A. F. 1971; Transport of aromatic amino acids by Pseudomonas aeruginosa. Journal of Bacteriology 105:1039–1046
    [Google Scholar]
  9. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  10. Martin R. G., Goldberger R. F. 1967; Imidazole acetolphosphate: l-glutamate aminotransferase. Purification and physical properties. Journal of Biological Chemistry 242:1168–1174
    [Google Scholar]
  11. Mee B. J., Lee B. T. O. 1967; Analysis of histidine requiring mutants of Pseudomonas aeruginosa. Genetics 55:709–722
    [Google Scholar]
  12. Mee B. J., Lee B. T. O. 1969; A map order of his-I, one of the genetic regions controlling histidine biosynthesis in Pseudomonas aeruginosa, using transducing phage F116. Genetics 62:687–696
    [Google Scholar]
  13. Tabor H., Hayaishi O. 1952; The enzymatic conversion of histidine to glutamic acid. Journal of Biological Chemistry 194:171–175
    [Google Scholar]
  14. Vogel H. J., Bonner D. M. 1956; Acetylornithinase of Escherichia coli: partial purification and some properties. Journal of Biological Chemistry 218:97–106
    [Google Scholar]
  15. Voll M. J., Apella E., Martin R. G. 1967; Purification and composition studies of phosphoribosyl- ATP: pyrophosphate phosphoribosyl transferase, the first enzyme of histidine biosynthesis. Journal of Biological Chemistry 242:1760–1767
    [Google Scholar]
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