1887

Abstract

SUMMARY: Two situations have been observed in which daughter strand gaps in DNA synthesized after exposure of excision-deficient Escherichia coli to ultraviolet light are filled but in which no mutations are formed as judged by loss of photoreversibility: (i) during the first 20 min of growth after u.v. irradiation, and (ii) when repair is allowed to ocfn in buffer. We suggest as an explanation that the majority of daughter strand gap-filling is error free and that mutations arise through a minor error-prone repair pathway which is inoperative under these conditions.

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/content/journal/micro/10.1099/00221287-91-2-369
1975-12-01
2024-05-02
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References

  1. Boyle J. M., Symonds N. 1969; Radiation-sensitive mutants of T4D. I. T4y; a new radiation-sensitive mutant; effect of the mutation on radiation survival, growth and recombination. Mutation Research 8:431–439
    [Google Scholar]
  2. Bridges B. A., Dennis R. E., Munson R. J. 1967; Differential induction and repair of ultraviolet damage leading to true reversions and external suppressor mutations of an ochre codon in Escherichia coli B/r wp2. Genetics 57:897–908
    [Google Scholar]
  3. Bridges B. A., Sedgwick S. G. 1974; Effect of photoreactivation on the filling of gaps in deoxyribonucleic acid synthesized after exposure of Escherichia coli to ultraviolet light. Journal of Bacteriology 117:1077–1081
    [Google Scholar]
  4. Davis B. D., Mingioli E. S. 1950; Mutants of Escherichia coli requiring methionine or vitamin B12. Journal of Bacteriology 60:17–28
    [Google Scholar]
  5. Defais M., Fauquet P., Radman M., Errera M. 1971; Ultraviolet reactivation and ultraviolet mutagenesis of λ in different genetic systems. Virology 43:495–503
    [Google Scholar]
  6. Devoret R. 1974; Mechanisms involved in the recovery of phage λ from UV damage. In Molecular Mechanisms for the repair of DNA. Hanawalt P. C., Setlow R. B. Edited by New York: Plenum Press;
    [Google Scholar]
  7. Doubleday O. P., Bridges B. A., Green M. H. L. 1975; Mutagenic DNA repair in Escherichia coli.II. Factors affecting loss of photoreversibility of UV-induced mutations. Molecular and General Genetics (in the Press)
    [Google Scholar]
  8. Green M. H. L., Gray W. J. H., Sedgwick S. G., Bridges B. A. 1973; Repair of DNA damage produced by gamma-radiation in Escherichia coli K-12 and a radiation-sensitive exrA derivative during inhibition of protein synthesis and normal DNA replication by chloramphenicol. Journal of General Microbiology 77:99–108
    [Google Scholar]
  9. Hill R. F. 1965; Ultraviolet-induced lethality and reversion to prototrophy in Escherichia coli strains with normal and reduced dark repair ability. Photochemistry and Photobiology 4:563–568
    [Google Scholar]
  10. Kondo S. 1973; Evidence that mutations are induced by errors in repair and replication. Genetics 73:109–122
    [Google Scholar]
  11. Lehmann A. R., Ormerod M. G. 1969; Artefact in the measurement of the molecular weight of pulse-labelled DNA. Nature; London: 2211053–1056
    [Google Scholar]
  12. McGrath R., Williams R. 1966; Reconstruction in vivo of irradiated Escherichia coli deoxyribonucleic acid; the rejoining of broken pieces. Nature; London: 212534–535
    [Google Scholar]
  13. Radman M. 1974; SOS repair: an inducible mutagenic DNA repair. In Molecular Mechanisms for the repair of DNA. Hanawalt P. C., Setlow R. B. Edited by New York: Plenum Press;
    [Google Scholar]
  14. Rupp W. D., Howard-Flanders P. 1968; Discontinuities in the DNA synthesized in an excision-defective strain of Escherichia coli following ultraviolet irradiation. Journal of Molecular Biology 31:291–304
    [Google Scholar]
  15. Rupp W. D., Wilde C. E. III Howard-Flanders P. 1971; Exchanges between DNA strands in ultraviolet-irradiated Escherichia coli. Journal of Molecular Biology 61:25–44
    [Google Scholar]
  16. Setlow R. B. 1967; Steps in the repair of DNA: fact and fancy. Brookhaven Symposia in Biology 20:1–16
    [Google Scholar]
  17. Smith K. C., Meun D. H. C. 1970; Repair of radiation-induced damage in Escherichia coli. I. Effect of mutations on post-replication repair of damage due to ultraviolet radiation. Journal of Molecular Biology 51:459–472
    [Google Scholar]
  18. Witkin E. M. 1967; Mutation-proof and mutation-prone modes of survival in derivatives of Escherichia coli b differing in sensitivity to ultraviolet light. Brookhaven Symposia in Biology 20:17–55
    [Google Scholar]
  19. Witkin E. M. 1969; The mutability toward ultraviolet light of recombination-deficient strains of Escherichia coli. Mutation Research 8:9–14
    [Google Scholar]
  20. Witkin E. M., George D. L. 1973; Ultraviolet mutagenesis in polA and uvrA polA derivatives of Escherichia coli B/r: evidence for an inducible error-prone repair system. Genetics 73:S91–108
    [Google Scholar]
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