Preliminary Characterization of Cell-free K99 Antigen Isolated from Escherichia coli B41
Morris, J. A. and Stevens, A. E. and Sojka, W. J.,, 99, 353-357 (1977),
doi = https://doi.org/10.1099/00221287-99-2-353,
publicationName = Microbiology Society,
issn = 1350-0872,
abstract= Summary: The K99 antigen of Escherichia coli b41 was isolated by isoelectric precipitation from heated bacterial suspensions. Chromatography and immunoabsorption experiments suggested that the mannose-resistant haemagglutinating activity of partially purified preparations of antigen was K99. The antigen was partially susceptible to bacterial proteases and was inactivated by periodate oxidation. Haemagglutination inhibition experiments with sugars and absorption of K99 with antisera to human blood groups A and B substances suggested that K99 contains a terminal α-linked N-acetylgalactosamine moiety, which is involved in the haemagglutination reaction, and an adjacent terminal α-linked galactose moiety, which plays no part in the reaction.,
language=,
type=