1887

Abstract

The psychrotrophic bacterium, strain MFO, is more sensitive to the -lactam mezlocillin at a low growth temperature (i.e. 8°C) than at a higher growth temperature (28°C). An early effect of this antibiotic at all temperatures is bacterial filamentation, but this occurs later at 8°C than at 28 °C, which suggests a lower permeability of the cell envelopes to mezlocillin at low growth temperature. -Lactamase production is later induced by mezlocillin, but the level of this induction also depends on the growth temperature, the overall induction being much less efficient at 8 °C. It is hypothesized that the periplasmic concentration of the drug might be too low at 8 °C to allow efficient -lactamase induction; this hypothesis was confirmed by the demonstration that -lactamase production is drastically enhanced in cells cultivated at 8°C permeabilized for 10 min by Na-EDTA. In addition, induction kinetic curves display a marked dependence upon growth temperature. A rapid saturation was evident when mezlocillin concentrations were increased at 8°C; this was not seen at 28 T°C at up to 1000 μg mezlocillin ml. The results are discussed in terms of two different routes of drug permeation, depending on the growth temperature.

Loading

Article metrics loading...

/content/journal/micro/10.1099/13500872-140-11-3125
1994-11-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/140/11/mic-140-11-3125.html?itemId=/content/journal/micro/10.1099/13500872-140-11-3125&mimeType=html&fmt=ahah

References

  1. Bavoil P., Nikaido H., Von Meyenburg K. 1977; Pleiotropic transport mutants of E. colilack porin, a major outer membrane protein. Mol and Gen Genet 158:23–33
    [Google Scholar]
  2. Bhakoo M., Herbert R. A. 1980; Fatty acid and phospholipid composition of five psychrotrophic Pseudomonasspp. growth at different temperatures. Arch Microbiol 126:51–55
    [Google Scholar]
  3. Bleschmidt B., Borneleit P., Emanouilidis I., Lehman W., Kleber H. P. 1989; Extracellular location of β-lactamase produced by Acinetobacter calcoaceticus . Appl Microbiol Biotechnol 32:85–89
    [Google Scholar]
  4. Gügi B., Orange N., Hellio F., Burini J. F., Guillou C., Leriche F., Guespin-Michel J. 1991; Effect of growth temperature on several exported enzyme activities in the psychrotrophic bacteria Pseudomonas fluorescens . J Bacteriol 173:3814–3820
    [Google Scholar]
  5. Hancock R.W.E. 1984; Alterations in the outer membrane permeability. Annu Rev Microbiol 56:395–411
    [Google Scholar]
  6. Hancock R.W.E. 1985; Effect of antibiotics on Pseudomonas.The Pseudomonas aeruginosaouter membrane permeability barrier and how to overcome it. Antibiot Chemother 36:95–102
    [Google Scholar]
  7. Hancock R.W.E. 1987; Role of porins in outer membrane permeability. J Bacteriol 169:929–933
    [Google Scholar]
  8. Hancock R.W.E. 1991; Bacterial outer membranes: evolving concepts. Specific structures provide Gram-negative bacteria with several unique advantages.. Amer Soc Microbiol News 57:175–181
    [Google Scholar]
  9. Hancock R.W.E., Siehnel R., Martin N. 1990; Outer membrane of Pseudomonas . Mol Microbiol 4:1069–1075
    [Google Scholar]
  10. Hellio F. C., Orange N., Guespin-Michel J. 1993; Temperature regulation of protease secretion by Pseudomonas fluorescensMFO.. Res Microbiol 144:617–626
    [Google Scholar]
  11. Mérieau A., Gügi B., Guespin-Michel J., Orange N. 1993; Temperature regulation of lipase secretion by Pseudomonas fluorescens . Appl Microbiol Biotechnol 39:104–109
    [Google Scholar]
  12. Mizuno T., Kageyama M. 1978; Separation and characterization of the outer membrane of Pseudomonas aeruginosa. . J Biochem 84:179–191
    [Google Scholar]
  13. Nicas T.I., Hancock W. R. E. 1983; Outer membrane permeability in Pseudomonas aeruginosa.Isolation of a porin protein F deficient mutant. J Bacteriol 153:281–285
    [Google Scholar]
  14. Nikaido H. 1985; Role of permeability barriers in resistance to beta-lactam antibiotics.. Pharmacol Ther 27:197–231
    [Google Scholar]
  15. Nikaido H. 1990; Permeability of the lipid domains of bacterial membranes.. In Membrane Transport and Information Storage pp. 165–190 Edited by Aloja R. C., Curtain C. C., Gordon L. M. New York: Alan R. Liss;
    [Google Scholar]
  16. Nikaido H., Vaara M. 1985; Molecular basis of bacterial outer membrane permeability. Microbiol Ren 49:1–32
    [Google Scholar]
  17. Nikaido H., Sony S. A., Shaltiel L., Nurminen M. 1979; Outer membrane of Salmonella.XIV. Reduced transmembrane diffusion rates in porin deficient mutants.. Biochem Biophys Rm Commun 76:324–330
    [Google Scholar]
  18. Park J.T. 1987; The murein sacculus. In Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology pp. 23–30 Edited by Neidhardt F. C., Ingraham J. L. , Low K. B. , Magasanik B., Schaechter M. , Umbarger H. E. . Washington, DC: American Society for Microbiology;
    [Google Scholar]
  19. Pfennig N., Wagener S. 1986; An improved method of preparing wet mounts for photomicrographs of microorganisms. J Microbiol Methods 4:303–306
    [Google Scholar]
  20. Russell N.J. 1990; Cold adaptation of microorganisms.. Phil Trans R Soc LondB 326:595–611
    [Google Scholar]
  21. Russell N.J., Fukunaga N. 1990; A comparison of thermal adaptation of membrane lipids in psychrotrophilic and thermophilic bacteria. Fems Microbiol Ren 75:171–182
    [Google Scholar]
  22. Sykes R.B., Nordström K. 1972; Microiodometric determination of beta-lactamase activity. Antimicrob Agents Chemother 1:94–99
    [Google Scholar]
  23. Vaara M. 1990; Agents that increase the permeability of the outer membrane. Microbiol Ren 56:395–411
    [Google Scholar]
  24. Yoshimura F., Nikaido H. 1985; Diffusion of beta-lactam antibiotics through the porin channel of Escherichia coliK-12. Antimicrob Agents Chemother 27:87–92
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-140-11-3125
Loading
/content/journal/micro/10.1099/13500872-140-11-3125
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error