1887

Abstract

Summary: Atypical oral isolates were recovered from 60 HIV-infected and three HIV-negative individuals. These organisms were germ-tube-positive and produced abundant chlamydospores which were frequently arranged in triplets or in contiguous pairs. They belonged to serotype A and had atypical carbohydrate assimilation profiles. Fingerprinting the genomic DNA of a selection of these organisms with the -specific probe 27A and five separate oligonucleotides, homologous to eukaryotic microsatellite repeat sequences, demonstrated that they had a very distinct genomic organization compared to and . This was further established by random amplified polymorphic DNA (RAPD) and karyotype analysis. Comparison of 500 bp of the V3 variable region of the large ribosomal subunit genes from nine atypical isolates and the corresponding sequences determined from and showed that the atypical organisms formed a homogeneous cluster (100% similarity) that was significantly different from the other species analysed, but was most closely related to and . These genetic data combined with the phenotypic characteristics of these atypical organisms strongly suggest that they constitute a novel species within the genus for which the name is proposed.

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1995-07-01
2024-03-29
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References

  1. Akisada T., Harada K., Niimi M., Kamaguchi A. 1983; Production of contiguously arranged chlamydospores in Candida albicans.. J Gen Microbiol 129:2327–2330
    [Google Scholar]
  2. Akopyanz N., Bukanov N.O., Westblom T.U., Kresovich S., Berg D.E. 1992; DNA diversity among clinical isolates of Helicobacter pylori detected by PCR-based RAPD fingerprinting.. Nucleic Acids Res 20:5137–5142
    [Google Scholar]
  3. Allen C.M., Beck F.M. 1983; Strain-related differences in pathogenicity of Candida albicans for oral mucosa.. J Infect Dis 147:1036–1040
    [Google Scholar]
  4. Barnett J.A., Payne R.W., Yarrow D.Y. 1990; Descriptions of the species arranged alphabetically.. In Yeasts: Characteristics and Identification, 2nd edn. pp. 79–695 Cambridge:: Cambridge University Press.;
    [Google Scholar]
  5. Buckley H.R. 1989; Identification of yeasts.. In Medical Mycology: A Practical Approach pp. 97–109 Evans E.G.V., Richardson M.D. Edited by Oxford:: IRL Press.;
    [Google Scholar]
  6. Check W.A. 1994; Molecular techniques shed light on fungal genetics.. ASM News 60:593–596
    [Google Scholar]
  7. Coleman D.C., Arbuthnott J.P., Pomeroy H.M., Birkbeck T.H. 1986; Cloning and expression in Escherichia coli and Staphylococcus aureus of the beta-lysin determinant from Staphylococcus aureus: evidence that bacteriophage conversion of beta-lysin activity is caused by insertional inactivation of the beta-lysin determinant.. Microb Pathogen 1:549–564
    [Google Scholar]
  8. Coleman D.C., Bennett D.E., Sullivan D.J., Gallagher P.J., Henman M.C., Shanley D.B., Russell R.J. 1993; Oral Candidain HIV infection and AIDS: new perspectives/new approaches.. Crit Rep Microbiol 19:61–82
    [Google Scholar]
  9. Durkacz B., Beach D., Hayles J., Nurse P. 1985; The fission yeast cell cycle control gene cdc2: structure of the cdc2 region.. Mol & Gen Genet 201:543–545
    [Google Scholar]
  10. Edman J.C., Kovacs A.J., Masur H., Santi D.V., Elwood H.J., Sogin M.L. 1988; Ribosomal RNA sequence shows Pneumocystis carinii to be a member of the fungi.. Nature 334:519–522
    [Google Scholar]
  11. Fell J.W. 1993; Rapid identification of yeast species using three primers in a polymerase chain reaction.. Mol Mar Biol Biotechnol 3:174–180
    [Google Scholar]
  12. Fell J.W., Statzell-Tallman A., Lutz M.R., Kurtzman C.P. 1992; Partial rRNA sequences in marine yeasts: a model for identification of marine eukaryotes.. Mol Mar Biol Biotechnol 1:175–186
    [Google Scholar]
  13. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap.. Evolution 39:783–791
    [Google Scholar]
  14. Gallagher P.J., Bennett D.E., Henman M.C., Russell R.J., Flint S.R., Shanley D.B., Coleman D.C. 1992; Reduced azole susceptibility of oral isolates of C. albicans from HIV-positive patients and a derivative exhibiting colony morphology variation.. J Gen Microbiol 138:1901–1911
    [Google Scholar]
  15. Ghannoum M.A., Swairjo I., Soll D.R. 1990; Variation in lipid and sterol contents in Candida albicans white and opaque phenotypes.. J Med Vet Mycol 28:103–115
    [Google Scholar]
  16. Jukes T.H., Cantor C.R. 1969; Evolution of protein molecules.. In Mammalian Protein Metabolism 3 pp. 21–132 Munro H.N. Edited by New York:: Academic Press.;
    [Google Scholar]
  17. Kamiyama A, Niimi M., Tokunaga M., Nakayama H. 1989; Adansonian study of Candida albicans: intraspecific homogeneity excepting C. stellatoidea strains.. J Med Vet Mycol 27:229–241
    [Google Scholar]
  18. Kwon-Chung K.J., Wickes B.L., Merz W.G. 1988; Association of electrophoretic karyotype of Candida stellatoidea with virulence for mice.. Infect Immun 56:1814–1819
    [Google Scholar]
  19. Kwon-Chung K.J., Riggsby W.S., Uphoff R.A., Hicks J.B., Whelan W.L., Reiss E., Magee B.B., Wickes B.L. 1989; Genetic differences between type I and type II Candida stellatoidea.. Infect Immun 57:527–532
    [Google Scholar]
  20. Larone D.H. 1993; Staining methods.. In Medically Important Fungi: A Guide to Identification pp. 186–192 Larone D.H. Edited by Washington, DC:: ASM.;
    [Google Scholar]
  21. McCullough M., Ross B., Reade P. 1995; Characterization of genetically distinct subgroup of Candida albicans strains isolated from oral cavities of patients infected with human immunodeficiency virus.. J Clin Microbiol 33:696–700
    [Google Scholar]
  22. Mahrous M., Lott T.J., Meyer S.A., Sawant A.D., Ahearn D.G. 1990; Electrophoretic karyotyping of typical and atypical Candida albicans.. J Clin Microbiol 28:876–881
    [Google Scholar]
  23. Marichal P., Gorrens J., Van Cutsem J., Van Den Bossche H. 1986; Culture media for the study of the effects of azole derivatives on germ tube formation and hyphal growth of C. albicans.. Mykosen 29:76–81
    [Google Scholar]
  24. Martinez J.P., Gill L., Casanova M., Ribot-Lopez J., De Lomas J.G., Sentandreu R. 1990; Wall mannoproteins in cells from colonial phenotypic variants of Candida albicans.. J Gen Microbiol 136:2421–2432
    [Google Scholar]
  25. Mercure S., Rougeau N., Montplaisir S., Lemay G. 1993; The nucleotide sequence of the 25S rRNA-encoding gene from Candida albicans.. Nucleic Acids Res 21:1490
    [Google Scholar]
  26. Odds F.C. 1988a; Pathogenesis of candidosis.. In Candida and Candidosis pp. 252–278 F. C. Odds F.C. Edited by London:: Baillière Tindall.;
    [Google Scholar]
  27. Odds F.C. 1988b; Biological aspects of pathogenic Candidaspecies.. In Candida and Candidosis pp. 7–15 Odds F.C. Edited by London:: Baillière Tindall.;
    [Google Scholar]
  28. Odds F.C. 1988c; Morphogenesis in Candida, with special reference to Candida albicans. . In Candida and Candidosis pp. 42–59 Odds F.C. Edited by London:: Baillière Tindall.;
    [Google Scholar]
  29. Pearce M.A., Howell S.A. 1991; Restriction fragment length polymorphism analysis of azole-resistant and azole-susceptible Candida albicans strains.. J Clin Microbiol 29:1364–1367
    [Google Scholar]
  30. Saitou N., Nei M. 1987; The neighbour-joining method: a new method for reconstructing phylogenetic trees.. Mol Biol Evol 4:406–425
    [Google Scholar]
  31. Sambrook J., Fritsch E.F., Maniatis T. 1989 Molecular Cloning: A Eaboratory Manual. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  32. Scherer S., Stevens D.A. 1988; A Candida albicans dispersed, repeated gene family and its epidemiologic applications.. Proc Natl AcadSci USA 851452–1456
    [Google Scholar]
  33. Schmid J., Odds F.C., Wiselka M.J., Nicholson K.G., Soll D.R. 1992; Genetic similarity and maintenance of Candida albicansstrains from a group of AIDS patients, demonstrated by DNA fingerprinting.. J Clin Microbiol 30:935–941
    [Google Scholar]
  34. Slutsky B., Staebell M., Anderson J., Risen L., Pfaller M., Soll D.R. 1987; ̒White-opaque transition̓: a second high-frequency switching system in Candida albicans.. J Bacteriol 169:189–197
    [Google Scholar]
  35. Southern E.M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis.. J Mol Biol 98:503–517
    [Google Scholar]
  36. Srikantha T., Gutell R.R., Morrow B., Soli D.R. 1994; Partial nucleotide sequence of a single ribosomal RNA coding region and secondary structure of the large subunit 25S rRNA of Candida albicans.. Curr Genet 26:321–328
    [Google Scholar]
  37. Sullivan D., Bennett D., Henman M., Harwood P., Flint S., Mulcahy F., Shanley D., Coleman D. 1993; Oligonucleotide fingerprinting of isolates of Candida species other than C. albicansand of atypical Candida species from human immunodeficiency virus-positive and AIDS patients.. J Clin Microbiol 31:2124–2133
    [Google Scholar]
  38. Thompson J.D., Higgins D.G., Gibson T.J. 1994; CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.. Nucleic Acids Res 22:4673–4680
    [Google Scholar]
  39. Vazquez J.A., Beckley A., Sobel J.D., Zervo M.J. 1991; Comparison of restriction enzyme analysis and pulsed-field gradient gel electrophoresis as typing systems for Candida albicans.. J Clin Microbiol 29:962–967
    [Google Scholar]
  40. Wakefield A.E., Peters S.E., Banerji S., Bridge P.D., Hall G.S., Hawksworth D.L., Guiver L.A., Allen A.G., Hopkin J.M. 1992; Pneumocystis carinii shows DNA homology with the ustomycetous red yeast fungi.. Mol Microbiol 6:1903–1911
    [Google Scholar]
  41. Wickes B.L., Golin J.E., Kwon-Chung K.J. 1991; Chromosomal rearrangement in Candida stellatoidea results in a positive effect on phenotype.. Infect Immun 59:1762–1771
    [Google Scholar]
  42. Williamson M.I., Samaranayake L.P., MacFarlane T.W. 1986; Biotypes of oral Candida albicans and Candida tropicalisisolates.. J Med Vet Mycol 24:81–84
    [Google Scholar]
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