1887

Abstract

A DNA contig of 26·2 kb covering the 170° region of the strain 168 genome was isolated and sequenced. For DNA isolation, suitable restriction sites at the end of previously known genes were chosen to amplify adjacent unknown DNA regions by inverse PCR. On the basis of the DNA sequence, 26 ORFs were identified of which and as well as parts of and have been described previously. Here we report the complete sequences of the aconitase () and transketolase () genes. Of the other proteins encoded on the 26·2 kb fragment, eight revealed similarities to previously described proteins. These included a pair of newly identified DNA gyrase subunits A () and B (), a sodium/proton-dependent alanine carrier (), a member of the thioredoxin family (), an endo-1,4--xylanase () and a response regulator protein. Comparison of the physical and the genetic maps revealed several differences. According to its flanking sequences the gene which was previously mapped at 162° was found to be adjacent to localized at 170°. Genes and were located the opposite way round and closer together than expected from the genetic map ( at 173° and at 170°). The gene, which was mapped at 169°, was not present on the respective fragment. Sequence comparison actually showed that is located close to 70° on the genome.

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1996-11-01
2024-04-19
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References

  1. Bairoch A., Bucher P. 1994; PROSITE: recent developments. Nucleic Acids Res 22:3583–3589
    [Google Scholar]
  2. Bischoff D. S., Ordal G. W. 1991; Sequence and characterization of Bacillus subtilis CheB, a homolog of the Escherichia coliCheY, and its role in a different mechanism of chemotaxis. J Biot Chem 266:12301–12305
    [Google Scholar]
  3. Dingman D., Sonenshein A. L. 1987; Purification of aconitase from Bacillus subtilis and correlation of its N-terminal amino acid sequence with the sequence of the citB gene. J Bacteriol 169:3062–3067
    [Google Scholar]
  4. Fischer C., Geourjon C., Bourson C., Deutscher J. 1996; Cloning and characterization of the Bacillus subtilis prkA gene encoding a novel serine protein kinase. Gene 168:55–60
    [Google Scholar]
  5. Fleischmann R. D. and others 1995; Whole-genome random sequencing and assembly of Haemophilus influenzae Rd. Science 269:496–512
    [Google Scholar]
  6. Fraser C. M. and others 1995; The minimal gene complement of Mycoplasma genitalium. Science 270:397–403
    [Google Scholar]
  7. Gosalbes M. J., Perez-Gonzalez J. A., Gonzalez R., Navarro A. 1991; Two β-glycanase genes are clustered in Bacillus polymyxa-. molecular cloning, expression and sequence analysis of genes encoding a xylanase and an endo-β-(l,3)-(l,4)-glucanase. J Bacteriol 173:7705–7710
    [Google Scholar]
  8. Grepinet O., Chebrou M. G., Beguin P. 1988; Nucleotide sequence and deletion analysis of the xylanase gene (xynZ) of Clostridium thermocellum. J Bacteriol 170:4582–4588
    [Google Scholar]
  9. Kamata H., Akiyama S., Morosawa H., Ohta T., Hamamoto T., Kambe T., Kgawa Y., Hirata H. 1992; Primary structure of the alanine carrier protein of thermophilic bacterium PS3. J Biol Chem 267:21650–21655
    [Google Scholar]
  10. Loferer H., Bott M., Hennecke H. 1993; Bradyrhizobium japonicum TlpA, a novel membrane-anchored thioredoxin-like protein involved in the biogenesis of cytochrome aazand de-velopment of symbiosis. EMBO J 12:3373–3383
    [Google Scholar]
  11. MacKay R. M., Lo A., Willick G., Zuker M., Baird S., Dove M., Moranelli F., Seligy V. 1986; Structure of a Bacillus subtilis endo- β-l,4-glucanase gene. Nucleic Acids Res 14:9159–9179
    [Google Scholar]
  12. MacLeod P. R., MacLeod R. A. 1992; Identification and sequence of a Na (-f-)-linked gene from the marine bacterium Altermonas haloplanktis which functionally complements the dagA gene of Escherichia coli. Mol Microbiol 6:2673–2681
    [Google Scholar]
  13. Moriya S., Ogasawara N., Yoshikawa H. 1985; Structure and function of the region of the replication origin of the Bacillus subtilis chromosome. III. Nucleotide sequence of some 10,000 base pairs in the origin region. Nucleic Acids Res 13:2251–2265
    [Google Scholar]
  14. Raymond-Denise A., Guillen N. 1991; Identification of dinR, a DNA damage-inducible regulator gene of Bacillus subtilis. J Bacteriol 173:7084–7091
    [Google Scholar]
  15. Sakka K., Kojima Y., Kondo T., Karita S., Ohmiya K., Shimada K. 1993; Nucleotide sequence of the Clostridium stercorarium xynA gene encoding xylanases A: identification of catalytic and cellulose binding domains. Biosci Biotechnol Biochem 57:273–277
    [Google Scholar]
  16. Wolf M., Geczi A., Simon O., Borriss R. 1995; Genes encoding xylan and β-glucan hydrolysing enzymes in Bacillus subtilis-. characterization, mapping and construction of strains deficient in lichenase, cellulase and xylanase. Microbiology 141:281–290
    [Google Scholar]
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