%0 Journal Article %A Reizer, Jonathan %A Ramseier, Tom M. %A Reizer, Aiala %A Charbit, Alain %A Saier, Milton H. %T Novel phosphotransferase genes revealed by bacterial genome sequencing: a gene cluster encoding a putative N-acetylgalactosamine metabolic pathway in Escherichia coli %D 1996 %J Microbiology, %V 142 %N 2 %P 231-250 %@ 1465-2080 %R https://doi.org/10.1099/13500872-142-2-231 %K phosphotransferase system %K sugar transport %K Escherichia coligenome %K aldolases %K N-acetylgalacrosamine %I Microbiology Society, %X Summary: We have analysed a gene cluster in the 67.4-76.0 min region of the Escherichia coli chromosome, revealed by recent systematic genome sequencing. The genes within this cluster include: (1) five genes encoding homologues of the E. coli mannose permease of the phosphotransferase system (IIB, IIB', IIC, IIC' and IID); (2) genes encoding a putative N-acetylgalactosamine 6-phosphate metabolic pathway including (a) a deacetylase, (b) an isomerizing deaminase, (c) a putative carbohydrate kinase, and (d) an aldolase; and (3) a transcriptional regulatory protein homologous to members of the DeoR family. Evidence is presented suggesting that the aldolase-encoding gene within this cluster is the previously designated kba gene that encodes tagatose-1,6-bisphosphate aldolase. These proteins and a novel IIAMan-like protein encoded in the 2.4-4.1 min region are characterized with respect to their sequence similarities and phylogenetic relationships with other homologous proteins. A pathway for the metabolism of N-acetylgalactosamine biochemically similar to that for the metabolism of N-acetylglucosamine is proposed. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/13500872-142-2-231